Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1997 Nov;35(11):2791–2794. doi: 10.1128/jcm.35.11.2791-2794.1997

Inhibitory effects of polyoxyethylene stearate, PANTA, and neutral pH on growth of Mycobacterium genavense in BACTEC primary cultures.

L Realini 1, P Van Der Stuyft 1, K De Ridder 1, B Hirschel 1, F Portaels 1
PMCID: PMC230063  PMID: 9350735

Abstract

We report on the influences of polyoxyethylene stearate (POES), PANTA, and pH on primary cultures of Mycobacterium genavense in BACTEC vials. As a model for primary cultures from tissue, seven different strains first isolated from AIDS patients (five from Switzerland and two from the United States) were inoculated into nude mice in order to obtain large amounts of bacilli to test different conditions simultaneously. Our results demonstrate that the size of the inoculum (10[6] acid-fast bacilli/vial), an acid pH (pH 6.0), and the absence of additives (POES and PANTA) significantly (P < 0.001) increased the probability of a successful culture in 1 month, considering growth index (GI) of > or =100 or a GI of > or =999 as criterion of success. In logistic regression analysis, all factors maintained a significant (P < 0.001) independent effect, and no interactions were observed between them. The best conditions for the primary cultures of M. genavense were the use of Middlebrook 7H12 medium at pH 6.0 without any additives.

Full Text

The Full Text of this article is available as a PDF (238.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bessesen M. T., Shlay J., Stone-Venohr B., Cohn D. L., Reves R. R. Disseminated Mycobacterium genavense infection: clinical and microbiological features and response to therapy. AIDS. 1993 Oct;7(10):1357–1361. [PubMed] [Google Scholar]
  2. Böttger E. C., Hirschel B., Coyle M. B. Mycobacterium genavense sp. nov. Int J Syst Bacteriol. 1993 Oct;43(4):841–843. doi: 10.1099/00207713-43-4-841. [DOI] [PubMed] [Google Scholar]
  3. Böttger E. C. Mycobacterium genavense: an emerging pathogen. Eur J Clin Microbiol Infect Dis. 1994 Nov;13(11):932–936. doi: 10.1007/BF02111494. [DOI] [PubMed] [Google Scholar]
  4. Böttger E. C., Teske A., Kirschner P., Bost S., Chang H. R., Beer V., Hirschel B. Disseminated "Mycobacterium genavense" infection in patients with AIDS. Lancet. 1992 Jul 11;340(8811):76–80. doi: 10.1016/0140-6736(92)90397-l. [DOI] [PubMed] [Google Scholar]
  5. Camargo E. E., Kertcher J. A., Larson S. M., Tepper B. S., Wagner H. N., Jr Radiometric measurement of differential metabolism of fatty acid by mycobacteria. Int J Lepr Other Mycobact Dis. 1982 Jun;50(2):200–204. [PubMed] [Google Scholar]
  6. Conville P. S., Andrews J. W., Witebsky F. G. Effect of PANTA on growth of Mycobacterium kansasii in BACTEC 12B medium. J Clin Microbiol. 1995 Aug;33(8):2012–2015. doi: 10.1128/jcm.33.8.2012-2015.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Coyle M. B., Carlson L. C., Wallis C. K., Leonard R. B., Raisys V. A., Kilburn J. O., Samadpour M., Böttger E. C. Laboratory aspects of "Mycobacterium genavense," a proposed species isolated from AIDS patients. J Clin Microbiol. 1992 Dec;30(12):3206–3212. doi: 10.1128/jcm.30.12.3206-3212.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cutler R. R., Wilson P., Clarke F. V. The effect of polyoxyethylene stearate (POES) on the growth of mycobacteria in radiometric 7H12 Middlebrook TB medium. Tubercle. 1987 Sep;68(3):209–220. doi: 10.1016/0041-3879(87)90057-2. [DOI] [PubMed] [Google Scholar]
  9. Franzblau S. G. Drug susceptibility testing of Mycobacterium leprae in the BACTEC 460 system. Antimicrob Agents Chemother. 1989 Dec;33(12):2115–2117. doi: 10.1128/aac.33.12.2115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hirschel B., Chang H. R., Mach N., Piguet P. F., Cox J., Piguet J. D., Silva M. T., Larsson L., Klatser P. R., Thole J. E. Fatal infection with a novel, unidentified mycobacterium in a man with the acquired immunodeficiency syndrome. N Engl J Med. 1990 Jul 12;323(2):109–113. doi: 10.1056/NEJM199007123230207. [DOI] [PubMed] [Google Scholar]
  11. Jackson K., Sievers A., Ross B. C., Dwyer B. Isolation of a fastidious Mycobacterium species from two AIDS patients. J Clin Microbiol. 1992 Nov;30(11):2934–2937. doi: 10.1128/jcm.30.11.2934-2937.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Miller M. A., Thibert L., Desjardins F., Siddiqi S. H., Dascal A. Growth inhibition of Mycobacterium tuberculosis by polyoxyethylene stearate present in the BACTEC pyrazinamide susceptibility test. J Clin Microbiol. 1996 Jan;34(1):84–86. doi: 10.1128/jcm.34.1.84-86.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pechère M., Opravil M., Wald A., Chave J. P., Bessesen M., Sievers A., Hein R., von Overbeck J., Clark R. A., Tortoli E. Clinical and epidemiologic features of infection with Mycobacterium genavense. Swiss HIV Cohort Study. Arch Intern Med. 1995 Feb 27;155(4):400–404. doi: 10.1001/archinte.1995.00430040074009. [DOI] [PubMed] [Google Scholar]
  14. Portaels F., Pattyn S. R. Growth of mycobacteria in relation to the pH of the medium. Ann Microbiol (Paris) 1982 Sep-Oct;133(2):213–221. [PubMed] [Google Scholar]
  15. Shafran S. D., Taylor G. D., Talbot J. A. Disseminated Mycobacterium genavense infection in Canadian AIDS patients. Tuber Lung Dis. 1995 Apr;76(2):168–170. doi: 10.1016/0962-8479(95)90562-6. [DOI] [PubMed] [Google Scholar]
  16. Shepard C. C., McRae D. H. A method for counting acid-fast bacteria. Int J Lepr Other Mycobact Dis. 1968 Jan-Mar;36(1):78–82. [PubMed] [Google Scholar]
  17. Siddiqi S. H., Laszlo A., Butler W. R., Kilburn J. O. Bacteriologic investigations of unusual mycobacteria isolated from immunocompromised patients. Diagn Microbiol Infect Dis. 1993 May-Jun;16(4):321–323. doi: 10.1016/0732-8893(93)90083-j. [DOI] [PubMed] [Google Scholar]
  18. Tortoli E., Simonetti M. T., Dionisio D., Meli M. Cultural studies on two isolates of "Mycobacterium genavense" from patients with acquired immunodeficiency syndrome. Diagn Microbiol Infect Dis. 1994 Jan;18(1):7–12. doi: 10.1016/0732-8893(94)90127-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES