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. 1997 Dec;35(12):3058–3061. doi: 10.1128/jcm.35.12.3058-3061.1997

Use of serum-specific immunoglobulins A and G for detection of Helicobacter pylori infection in patients with chronic gastritis by immunoblot analysis.

S Karvar 1, H Karch 1, M Frosch 1, W Burghardt 1, U Gross 1
PMCID: PMC230122  PMID: 9399494

Abstract

Multiple invasive and noninvasive tests for detecting Helicobacter pylori infection are available. The current "gold standard" for the diagnosis of H. pylori infection requires histology and the rapid urease test. Our aim was to test the performance of immunoglobulin A (IgA) and IgG immunoblot assays in comparison with that of the gold standard tests for the diagnosis of H. pylori infection. Ninety patients who underwent gastroscopy were analyzed in a prospective study. Fifty-nine of them were defined to be H. pylori positive by the gold standard tests. The IgA and IgG immunoblot assays correctly identified H. pylori infection in 17 and 58 of these patients, respectively, indicating that determination of IgA antibodies seems to be of low diagnostic value for H. pylori infection. In contrast, the sensitivity and specificity of the IgG immunoblot assay were 98 and 71%, respectively, with positive and negative predictive values of 87 and 96%, respectively. Therefore, the IgG immunoblot assay proved to be a sensitive and useful, noninvasive test for the diagnosis of H. pylori infection.

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Selected References

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  1. Allerberger F., Oberhuber G., Wrba F., Püspök A., Dejaco C., Dierich M. P. Detection of Helicobacter pylori infection using single serum specimens: comparison of five commercial serological tests. Hepatogastroenterology. 1996 Nov-Dec;43(12):1656–1659. [PubMed] [Google Scholar]
  2. Andersen L. P., Espersen F. Immunoglobulin G antibodies to Helicobacter pylori in patients with dyspeptic symptoms investigated by the western immunoblot technique. J Clin Microbiol. 1992 Jul;30(7):1743–1751. doi: 10.1128/jcm.30.7.1743-1751.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Apel I., Jacobs E., Kist M., Bredt W. Antibody response of patients against a 120 kDa surface protein of Campylobacter pylori. Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Apr;268(2):271–276. doi: 10.1016/s0176-6724(88)80012-9. [DOI] [PubMed] [Google Scholar]
  4. Azuma T., Kato T., Hirai M., Ito S., Kohli Y. Diagnosis of Helicobacter pylori infection. J Gastroenterol Hepatol. 1996 Jul;11(7):662–669. doi: 10.1111/j.1440-1746.1996.tb00311.x. [DOI] [PubMed] [Google Scholar]
  5. Cutler A. F., Havstad S., Ma C. K., Blaser M. J., Perez-Perez G. I., Schubert T. T. Accuracy of invasive and noninvasive tests to diagnose Helicobacter pylori infection. Gastroenterology. 1995 Jul;109(1):136–141. doi: 10.1016/0016-5085(95)90278-3. [DOI] [PubMed] [Google Scholar]
  6. Faigel D. O., Childs M., Furth E. E., Alavi A., Metz D. C. New noninvasive tests for Helicobacter pylori gastritis. Comparison with tissue-based gold standard. Dig Dis Sci. 1996 Apr;41(4):740–748. doi: 10.1007/BF02213130. [DOI] [PubMed] [Google Scholar]
  7. Faigel D. O., Furth E. E., Childs M., Goin J., Metz D. C. Histological predictors of active Helicobacter pylori infection. Dig Dis Sci. 1996 May;41(5):937–943. doi: 10.1007/BF02091534. [DOI] [PubMed] [Google Scholar]
  8. Farinati F., Valiante F., Germanà B., Della Libera G., Baffa R., Rugge M., Plebani M., Vianello F., Di Mario F., Naccarato R. Prevalence of Helicobacter pylori infection in patients with precancerous changes and gastric cancer. Eur J Cancer Prev. 1993 Jul;2(4):321–326. doi: 10.1097/00008469-199307000-00006. [DOI] [PubMed] [Google Scholar]
  9. Gross U., Roos T., Appoldt D., Heesemann J. Improved serological diagnosis of Toxoplasma gondii infection by detection of immunoglobulin A (IgA) and IgM antibodies against P30 by using the immunoblot technique. J Clin Microbiol. 1992 Jun;30(6):1436–1441. doi: 10.1128/jcm.30.6.1436-1441.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Heesemann J., Eggers C., Schröder J. Serological diagnosis of yersiniosis by immunoblot technique using virulence-associated antigen of enteropathogenic Yersiniae. Contrib Microbiol Immunol. 1987;9:285–289. [PubMed] [Google Scholar]
  11. Ito Y., Azuma T., Ito S., Miyaji H., Hirai M., Yamazaki Y., Sato F., Kato T., Kohli Y., Kuriyama M. Analysis and typing of the vacA gene from cagA-positive strains of Helicobacter pylori isolated in Japan. J Clin Microbiol. 1997 Jul;35(7):1710–1714. doi: 10.1128/jcm.35.7.1710-1714.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Johansen H. K., Nørgaard A., Andersen L. P., Jensen P., Nielsen H., Høiby N. Cross-reactive antigens shared by Pseudomonas aeruginosa, Helicobacter pylori, Campylobacter jejuni, and Haemophilus influenzae may cause false-positive titers of antibody to H. pylori. Clin Diagn Lab Immunol. 1995 Mar;2(2):149–155. doi: 10.1128/cdli.2.2.149-155.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kist M., Spiegelhalder C., Moriki T., Schaefer H. E. Interaction of Helicobacter pylori (strain 151) and Campylobacter coli with human peripheral polymorphonuclear granulocytes. Zentralbl Bakteriol. 1993 Sep;280(1-2):58–72. doi: 10.1016/s0934-8840(11)80941-2. [DOI] [PubMed] [Google Scholar]
  14. Kuipers E. J., Uyterlinde A. M., Peña A. S., Roosendaal R., Pals G., Nelis G. F., Festen H. P., Meuwissen S. G. Long-term sequelae of Helicobacter pylori gastritis. Lancet. 1995 Jun 17;345(8964):1525–1528. doi: 10.1016/s0140-6736(95)91084-0. [DOI] [PubMed] [Google Scholar]
  15. Kurata J. H., Nogawa A. N. Meta-analysis of risk factors for peptic ulcer. Nonsteroidal antiinflammatory drugs, Helicobacter pylori, and smoking. J Clin Gastroenterol. 1997 Jan;24(1):2–17. doi: 10.1097/00004836-199701000-00002. [DOI] [PubMed] [Google Scholar]
  16. Labenz J., Bärsch G., Peitz U., Aygen S., Hennemann O., Tillenburg B., Becker T., Stolte M. Validity of a novel biopsy urease test (HUT) and a simplified 13C-urea breath test for diagnosis of Helicobacter pylori infection and estimation of the severity of gastritis. Digestion. 1996 Nov-Dec;57(6):391–397. doi: 10.1159/000201366. [DOI] [PubMed] [Google Scholar]
  17. Malfertheiner P., Enrique Domínguez-Muñoz J., Heckenmüller H., Neubrand M., Fischer H. P., Sauerbruch T. Modified rapid urease test for detection of Helicobacter pylori infection. Eur J Gastroenterol Hepatol. 1996 Jan;8(1):53–56. doi: 10.1097/00042737-199601000-00010. [DOI] [PubMed] [Google Scholar]
  18. Mégraud F. Advantages and disadvantages of current diagnostic tests for the detection of Helicobacter pylori. Scand J Gastroenterol Suppl. 1996;215:57–62. doi: 10.3109/00365529609094536. [DOI] [PubMed] [Google Scholar]
  19. Nilsson I., Ljungh A., Aleljung P., Wadström T. Immunoblot assay for serodiagnosis of Helicobacter pylori infections. J Clin Microbiol. 1997 Feb;35(2):427–432. doi: 10.1128/jcm.35.2.427-432.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Parsonnet J., Friedman G. D., Vandersteen D. P., Chang Y., Vogelman J. H., Orentreich N., Sibley R. K. Helicobacter pylori infection and the risk of gastric carcinoma. N Engl J Med. 1991 Oct 17;325(16):1127–1131. doi: 10.1056/NEJM199110173251603. [DOI] [PubMed] [Google Scholar]
  21. Parsonnet J., Hansen S., Rodriguez L., Gelb A. B., Warnke R. A., Jellum E., Orentreich N., Vogelman J. H., Friedman G. D. Helicobacter pylori infection and gastric lymphoma. N Engl J Med. 1994 May 5;330(18):1267–1271. doi: 10.1056/NEJM199405053301803. [DOI] [PubMed] [Google Scholar]
  22. Peura D. A. Helicobacter pylori: a diagnostic dilemma and a dilemma of diagnosis. Gastroenterology. 1995 Jul;109(1):313–315. doi: 10.1016/0016-5085(95)90299-6. [DOI] [PubMed] [Google Scholar]
  23. Plebani M., Basso D., Cassaro M., Brigato L., Scrigner M., Toma A., Di Mario F., Rugge M. Helicobacter pylori serology in patients with chronic gastritis. Am J Gastroenterol. 1996 May;91(5):954–958. [PubMed] [Google Scholar]
  24. Stolte M., Eidt S. Helicobacter pylori and the evolution of gastritis. Scand J Gastroenterol Suppl. 1996;214:13–23. [PubMed] [Google Scholar]
  25. Thijs J. C., van Zwet A. A., Thijs W. J., Oey H. B., Karrenbeld A., Stellaard F., Luijt D. S., Meyer B. C., Kleibeuker J. H. Diagnostic tests for Helicobacter pylori: a prospective evaluation of their accuracy, without selecting a single test as the gold standard. Am J Gastroenterol. 1996 Oct;91(10):2125–2129. [PubMed] [Google Scholar]
  26. Valle J., Kekki M., Sipponen P., Ihamäki T., Siurala M. Long-term course and consequences of Helicobacter pylori gastritis. Results of a 32-year follow-up study. Scand J Gastroenterol. 1996 Jun;31(6):546–550. doi: 10.3109/00365529609009126. [DOI] [PubMed] [Google Scholar]
  27. Weel J. F., van der Hulst R. W., Gerrits Y., Roorda P., Feller M., Dankert J., Tytgat G. N., van der Ende A. The interrelationship between cytotoxin-associated gene A, vacuolating cytotoxin, and Helicobacter pylori-related diseases. J Infect Dis. 1996 May;173(5):1171–1175. doi: 10.1093/infdis/173.5.1171. [DOI] [PubMed] [Google Scholar]

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