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. 1997 Dec;35(12):3303–3304. doi: 10.1128/jcm.35.12.3303-3304.1997

Surveillance of cytomegalovirus after solid-organ transplantation: comparison of pp65 antigenemia assay with a quantitative DNA hybridization assay.

W Bossart 1, K Bienz 1, W Wunderli 1
PMCID: PMC230168  PMID: 9399540

Abstract

In a multicenter study, 113 blood samples from 19 organ transplant patients were analyzed for cytomegalovirus by the pp65 antigenemia assay and a quantitative DNA hybridization assay. Overall, there was 84% agreement among the results obtained by the two tests. Fifteen of 16 episodes of active infection were detected by both assays. One episode was missed by the pp65 assay, and one patient showed significant DNA-emia but only low-level antigenemia.

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Selected References

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  1. Bailey T. C. Prevention of cytomegalovirus disease. Semin Respir Infect. 1993 Sep;8(3):225–232. [PubMed] [Google Scholar]
  2. Gerna G., Zipeto D., Parea M., Revello M. G., Silini E., Percivalle E., Zavattoni M., Grossi P., Milanesi G. Monitoring of human cytomegalovirus infections and ganciclovir treatment in heart transplant recipients by determination of viremia, antigenemia, and DNAemia. J Infect Dis. 1991 Sep;164(3):488–498. doi: 10.1093/infdis/164.3.488. [DOI] [PubMed] [Google Scholar]
  3. Griffiths P. D. Current management of cytomegalovirus disease. J Med Virol. 1993;Suppl 1:106–111. doi: 10.1002/jmv.1890410521. [DOI] [PubMed] [Google Scholar]
  4. Gómez E., de Oña M., Aguado S., Tejada F., Nuñez M., Portal C., Diaz-Corte C., Sanchez E., Ortega F., Alvarez-Grande J. Cytomegalovirus preemptive therapy with ganciclovir in renal transplant patients treated with OKT3. Nephron. 1996;74(2):367–372. doi: 10.1159/000189337. [DOI] [PubMed] [Google Scholar]
  5. Johnson S. C., Stamm C. P., Hicks C. B. Tuberculous psoas muscle abscess following chemoprophylaxis with isoniazid in a patient with human immunodeficiency virus infection. Rev Infect Dis. 1990 Sep-Oct;12(5):754–756. doi: 10.1093/clinids/12.5.754. [DOI] [PubMed] [Google Scholar]
  6. Landry M. L., Ferguson D. Comparison of quantitative cytomegalovirus antigenemia assay with culture methods and correlation with clinical disease. J Clin Microbiol. 1993 Nov;31(11):2851–2856. doi: 10.1128/jcm.31.11.2851-2856.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lazzarotto T., Campisi T., Dal Monte P., Galli S., Spezzacatena P., Guglielmi P., Landini M. P. A quantitative test (HCMV-hybrid-capture(TM)) to detect human cytomegalovirus DNA in the blood of immunocompromised patients compared with antigenemia and polymerase chain reaction. New Microbiol. 1996 Jul;19(3):193–201. [PubMed] [Google Scholar]
  8. Mazzulli T., Wood S., Chua R., Walmsley S. Evaluation of the Digene Hybrid Capture System for detection and quantitation of human cytomegalovirus viremia in human immunodeficiency virus-infected patients. J Clin Microbiol. 1996 Dec;34(12):2959–2962. doi: 10.1128/jcm.34.12.2959-2962.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Niubò J., Pérez J. L., Martínez-Lacasa J. T., García A., Roca J., Fabregat J., Gil-Vernet S., Martín R. Association of quantitative cytomegalovirus antigenemia with symptomatic infection in solid organ transplant patients. Diagn Microbiol Infect Dis. 1996 Jan;24(1):19–24. doi: 10.1016/0732-8893(95)00248-0. [DOI] [PubMed] [Google Scholar]
  10. The T. H., van den Berg A. P., Harmsen M. C., van der Bij W., van Son W. J. The cytomegalovirus antigenemia assay: a plea for standardization. Scand J Infect Dis Suppl. 1995;99:25–29. [PubMed] [Google Scholar]
  11. The T. H., van der Ploeg M., van den Berg A. P., Vlieger A. M., van der Giessen M., van Son W. J. Direct detection of cytomegalovirus in peripheral blood leukocytes--a review of the antigenemia assay and polymerase chain reaction. Transplantation. 1992 Aug;54(2):193–198. doi: 10.1097/00007890-199208000-00001. [DOI] [PubMed] [Google Scholar]
  12. van Son W. J., van den Berg A. P., The T. H., Tegzess A. M. Preemptive therapy with gancyclovir for early high-risk CMV infection allows effective treatment with antithymocyte globulin of steroid-resistant rejection after renal transplantation. Transplant Proc. 1993 Feb;25(1 Pt 2):1436–1438. [PubMed] [Google Scholar]
  13. van den Berg A. P., Tegzess A. M., Scholten-Sampson A., Schirm J., van der Giessen M., The T. H., van Son W. J. Monitoring antigenemia is useful in guiding treatment of severe cytomegalovirus disease after organ transplantation. Transpl Int. 1992 May;5(2):101–106. doi: 10.1007/BF00339224. [DOI] [PubMed] [Google Scholar]

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