Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1997 Dec;35(12):3308–3310. doi: 10.1128/jcm.35.12.3308-3310.1997

Comparison of the InPouch TV culture system and Diamond's modified medium for detection of Trichomonas vaginalis.

M H Levi 1, J Torres 1, C Piña 1, R S Klein 1
PMCID: PMC230170  PMID: 9399542

Abstract

This study compared the use of Diamond's modified medium to InPouch for the culture of Trichomonas vaginalis from pooled vaginal secretions. The sensitivity for InPouch was 82.4% (61/74) versus 87.8% (65/74) for Diamond's modified medium. There were no significant differences in the sensitivity and negative predictive value of InPouch compared to Diamond's modified medium.

Full Text

The Full Text of this article is available as a PDF (113.7 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beal C., Goldsmith R., Kotby M., Sherif M., el-Tagi A., Farid A., Zakaria S., Eapen J. The plastic envelope method, a simplified technique for culture diagnosis of trichomoniasis. J Clin Microbiol. 1992 Sep;30(9):2265–2268. doi: 10.1128/jcm.30.9.2265-2268.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bhatt R., Pandit D., Deodhar L., Bhise R., Chatterjee D. K. Comparative pathogenicity of Trichomonas vaginalis isolated from symptomatic and asymptomatic cases. J Postgrad Med. 1993 Oct-Dec;39(4):187–189. [PubMed] [Google Scholar]
  3. Briselden A. M., Hillier S. L. Evaluation of affirm VP Microbial Identification Test for Gardnerella vaginalis and Trichomonas vaginalis. J Clin Microbiol. 1994 Jan;32(1):148–152. doi: 10.1128/jcm.32.1.148-152.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DeMeo L. R., Draper D. L., McGregor J. A., Moore D. F., Peter C. R., Kapernick P. S., McCormack W. M. Evaluation of a deoxyribonucleic acid probe for the detection of Trichomonas vaginalis in vaginal secretions. Am J Obstet Gynecol. 1996 Apr;174(4):1339–1342. doi: 10.1016/s0002-9378(96)70682-8. [DOI] [PubMed] [Google Scholar]
  5. Draper D., Parker R., Patterson E., Jones W., Beutz M., French J., Borchardt K., McGregor J. Detection of Trichomonas vaginalis in pregnant women with the InPouch TV culture system. J Clin Microbiol. 1993 Apr;31(4):1016–1018. doi: 10.1128/jcm.31.4.1016-1018.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gelbart S. M., Thomason J. L., Osypowski P. J., James J. A., Hamilton P. R. Comparison of Diamond's medium modified and Kupferberg medium for detection of Trichomonas vaginalis. J Clin Microbiol. 1989 May;27(5):1095–1096. doi: 10.1128/jcm.27.5.1095-1096.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gelbart S. M., Thomason J. L., Osypowski P. J., Kellett A. V., James J. A., Broekhuizen F. F. Growth of Trichomonas vaginalis in commercial culture media. J Clin Microbiol. 1990 May;28(5):962–964. doi: 10.1128/jcm.28.5.962-964.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Heine P., McGregor J. A. Trichomonas vaginalis: a reemerging pathogen. Clin Obstet Gynecol. 1993 Mar;36(1):137–144. doi: 10.1097/00003081-199303000-00019. [DOI] [PubMed] [Google Scholar]
  9. Klein R. S., Ho G. Y., Vermund S. H., Fleming I., Burk R. D. Risk factors for squamous intraepithelial lesions on Pap smear in women at risk for human immunodeficiency virus infection. J Infect Dis. 1994 Dec;170(6):1404–1409. doi: 10.1093/infdis/170.6.1404. [DOI] [PubMed] [Google Scholar]
  10. Krieger J. N., Verdon M., Siegel N., Holmes K. K. Natural history of urogenital trichomoniasis in men. J Urol. 1993 Jun;149(6):1455–1458. doi: 10.1016/s0022-5347(17)36414-5. [DOI] [PubMed] [Google Scholar]
  11. Minkoff H., Grunebaum A. N., Schwarz R. H., Feldman J., Cummings M., Crombleholme W., Clark L., Pringle G., McCormack W. M. Risk factors for prematurity and premature rupture of membranes: a prospective study of the vaginal flora in pregnancy. Am J Obstet Gynecol. 1984 Dec 15;150(8):965–972. doi: 10.1016/0002-9378(84)90392-2. [DOI] [PubMed] [Google Scholar]
  12. Paisarntantiwong R., Brockmann S., Clarke L., Landesman S., Feldman J., Minkoff H. The relationship of vaginal trichomoniasis and pelvic inflammatory disease among women colonized with Chlamydia trachomatis. Sex Transm Dis. 1995 Nov-Dec;22(6):344–347. doi: 10.1097/00007435-199511000-00004. [DOI] [PubMed] [Google Scholar]
  13. Provenzano D., Alderete J. F. Analysis of human immunoglobulin-degrading cysteine proteinases of Trichomonas vaginalis. Infect Immun. 1995 Sep;63(9):3388–3395. doi: 10.1128/iai.63.9.3388-3395.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Read J. S., Klebanoff M. A. Sexual intercourse during pregnancy and preterm delivery: effects of vaginal microorganisms. The Vaginal Infections and Prematurity Study Group. Am J Obstet Gynecol. 1993 Feb;168(2):514–519. doi: 10.1016/0002-9378(93)90484-z. [DOI] [PubMed] [Google Scholar]
  15. Royce R. A., Seña A., Cates W., Jr, Cohen M. S. Sexual transmission of HIV. N Engl J Med. 1997 Apr 10;336(15):1072–1078. doi: 10.1056/NEJM199704103361507. [DOI] [PubMed] [Google Scholar]
  16. Schmid G. P., Matheny L. C., Zaidi A. A., Kraus S. J. Evaluation of six media for the growth of Trichomonas vaginalis from vaginal secretions. J Clin Microbiol. 1989 Jun;27(6):1230–1233. doi: 10.1128/jcm.27.6.1230-1233.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Selwyn P. A., Alcabes P., Hartel D., Buono D., Schoenbaum E. E., Klein R. S., Davenny K., Friedland G. H. Clinical manifestations and predictors of disease progression in drug users with human immunodeficiency virus infection. N Engl J Med. 1992 Dec 10;327(24):1697–1703. doi: 10.1056/NEJM199212103272401. [DOI] [PubMed] [Google Scholar]
  18. Wasserheit J. N. Epidemiological synergy. Interrelationships between human immunodeficiency virus infection and other sexually transmitted diseases. Sex Transm Dis. 1992 Mar-Apr;19(2):61–77. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES