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. 1997 Dec;35(12):3316–3319. doi: 10.1128/jcm.35.12.3316-3319.1997

Serodiagnosis of American trypanosomosis by using nonpathogenic trypanosomatid antigen.

V M Monteón 1, L Guzmán-Rojas 1, C Negrete-García 1, J L Rosales-Encina 1, P A Lopez 1
PMCID: PMC230173  PMID: 9399545

Abstract

Crithidia luciliae, a nonpathogenic trypanosomatid, could provide a good alternative source of antigen for serodiagnosis of Chagas' disease. An enzyme-linked immunosorbent assay showed 100% sensitivity and 83% specificity when 91 human serum samples from Chagas' disease patients and 127 human serum samples from people suffering from toxoplasmosis (21 samples), leishmaniasis (32 samples), systemic rheumatic diseases (33 samples), and heart diseases (41 samples) were tested simultaneously with Trypanosoma cruzi and C. luciliae crude extracts. By Western blotting, an immunodominant band (30 kDa) was recognized by chagasic sera on the C. luciliae crude extract; specificity reached 97% with respect to this protein band. The carbohydrate moieties were not antigenic.

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Selected References

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  1. Bongertz V., Dvorak J. A. Trypanosoma cruzi: antigenic analysis of cloned stocks. Am J Trop Med Hyg. 1983 Jul;32(4):716–722. doi: 10.4269/ajtmh.1983.32.716. [DOI] [PubMed] [Google Scholar]
  2. Gloss G., Barrera M. R., Monteón V. M., Reyes P. A. Tripanosomiasis americana y cardiopatia chagásica crónica en el Instituto Nacional de Cardiologia Ignacio Chavez. Arch Inst Cardiol Mex. 1990 May-Jun;60(3):261–266. [PubMed] [Google Scholar]
  3. Kirchhoff L. V. American trypanosomiasis (Chagas' disease)--a tropical disease now in the United States. N Engl J Med. 1993 Aug 26;329(9):639–644. doi: 10.1056/NEJM199308263290909. [DOI] [PubMed] [Google Scholar]
  4. Lopes J. D., Caulada Z., Barbieri C. L., Camargo E. P. Cross-reactivity between Trypanosoma cruzi and insect trypanosomatids as a basis for the diagnosos of Chagas' disease. Am J Trop Med Hyg. 1981 Nov;30(6):1183–1188. doi: 10.4269/ajtmh.1981.30.1183. [DOI] [PubMed] [Google Scholar]
  5. McGhee R. B., Cosgrove W. B. Biology and physiology of the lower Trypanosomatidae. Microbiol Rev. 1980 Mar;44(1):140–173. doi: 10.1128/mr.44.1.140-173.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Moncayo A., Luquetti A. O. Multicentre double blind study for evaluation of Trypanosoma cruzi defined antigens as diagnostic reagents. Mem Inst Oswaldo Cruz. 1990 Oct-Dec;85(4):489–495. doi: 10.1590/s0074-02761990000400020. [DOI] [PubMed] [Google Scholar]
  7. Monteón V. M., Ramos A., Reyes P. A. Reactividad de sueros de pacientes chagásicos crónicos con extractos de aislamientos mexicanos de Trypanosoma cruzi. Rev Biol Trop. 1993 Dec;41(3B):861–865. [PubMed] [Google Scholar]
  8. Nagasse-Sugahara T. K., Hoshino-Shimizu S., Pagliarini R. C., Celeste B. J. Improvement of the slide hemagglutination test for rapid Chagas' disease screening in epidemiological surveys. Braz J Med Biol Res. 1996 May;29(5):623–628. [PubMed] [Google Scholar]
  9. Ramos-Echevarria A. A., Monteón-Padilla V. M., Reyes-López P. A. Detección de anticuerpos contra Trypanosoma cruzi en donadores de sangre. Salud Publica Mex. 1993 Jan-Feb;35(1):56–64. [PubMed] [Google Scholar]
  10. Rangel-Aldao R., Comach G., Allende O., Cayama E., Delgado V., Piras R., Piras M., Henriquez D., Negri S. Trypanosoma cruzi: polypeptide markers of epimastigotes and trypomastigotes. Mol Biochem Parasitol. 1986 Jul;20(1):25–32. doi: 10.1016/0166-6851(86)90139-8. [DOI] [PubMed] [Google Scholar]
  11. Reiche E. M., Inouye M. M., Pontello R., Morimoto H. K., Itow Jankevicius S., Matsuo T., Jankevicius J. V. Seropositivity for anti-trypanosoma cruzi antibodies among blood donors of the "Hospital Universitário Regional do Norte do Paraná", Londrina, Brazil. Rev Inst Med Trop Sao Paulo. 1996 May-Jun;38(3):233–240. doi: 10.1590/s0036-46651996000300014. [DOI] [PubMed] [Google Scholar]
  12. Umezawa E. S., Shikanai-Yasuda M. A., Stolf A. M. Changes in isotype composition and antigen recognition of anti-Trypanosoma cruzi antibodies from acute to chronic Chagas disease. J Clin Lab Anal. 1996;10(6):407–413. doi: 10.1002/(SICI)1098-2825(1996)10:6<407::AID-JCLA16>3.0.CO;2-0. [DOI] [PubMed] [Google Scholar]
  13. Vitor R. W., Chiari E. Avaliaço de antígenos do Trypanosoma cruzi para a reaço de hemaglutinaço indireta. I. Diferentes extratos antigênicos. Rev Inst Med Trop Sao Paulo. 1987 May-Jun;29(3):178–182. doi: 10.1590/s0036-46651987000300011. [DOI] [PubMed] [Google Scholar]
  14. Vitor R. W., Chiari E. Avaliaço de antígenos do Trypanosoma cruzi para e reaço de hemaglutinaço indireta. II. Antigenos de diferentes amostras e formas evolutivas. Rev Inst Med Trop Sao Paulo. 1987 May-Jun;29(3):183–188. doi: 10.1590/s0036-46651987000300012. [DOI] [PubMed] [Google Scholar]
  15. Woodward M. P., Young W. W., Jr, Bloodgood R. A. Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation. J Immunol Methods. 1985 Apr 8;78(1):143–153. doi: 10.1016/0022-1759(85)90337-0. [DOI] [PubMed] [Google Scholar]
  16. Xu B. X., Powell M. R. Carbohydrate epitopes are responsible for antibody cross-reactivity in Trypanosoma cruzi-infected mice. J Parasitol. 1991 Oct;77(5):808–810. [PubMed] [Google Scholar]

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