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. 1997 Dec;35(12):3331–3334. doi: 10.1128/jcm.35.12.3331-3334.1997

Rapid discrimination of Mycobacterium tuberculosis complex strains by ligation-mediated PCR fingerprint analysis.

G Prod'hom 1, C Guilhot 1, M C Gutierrez 1, A Varnerot 1, B Gicquel 1, V Vincent 1
PMCID: PMC230178  PMID: 9399550

Abstract

A ligation-mediated PCR (LMPCR) method for the amplification of sequences flanking the IS6110 of the Mycobacterium tuberculosis complex has been developed. The method uses one primer specific for IS6110 and a second specific for a linker ligated to SalI-restricted genomic DNA. LMPCR is a rapid screening method, valuable for the fingerprinting of M. tuberculosis complex strains.

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Selected References

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  1. Aranaz A., Liébana E., Mateos A., Dominguez L., Vidal D., Domingo M., Gonzolez O., Rodriguez-Ferri E. F., Bunschoten A. E., Van Embden J. D. Spacer oligonucleotide typing of Mycobacterium bovis strains from cattle and other animals: a tool for studying epidemiology of tuberculosis. J Clin Microbiol. 1996 Nov;34(11):2734–2740. doi: 10.1128/jcm.34.11.2734-2740.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Butler W. R., Haas W. H., Crawford J. T. Automated DNA fingerprinting analysis of Mycobacterium tuberculosis using fluorescent detection of PCR products. J Clin Microbiol. 1996 Jul;34(7):1801–1803. doi: 10.1128/jcm.34.7.1801-1803.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chaves F., Yang Z., el Hajj H., Alonso M., Burman W. J., Eisenach K. D., Dronda F., Bates J. H., Cave M. D. Usefulness of the secondary probe pTBN12 in DNA fingerprinting of Mycobacterium tuberculosis. J Clin Microbiol. 1996 May;34(5):1118–1123. doi: 10.1128/jcm.34.5.1118-1123.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Friedman C. R., Stoeckle M. Y., Johnson W. D., Jr, Riley L. W. Double-repetitive-element PCR method for subtyping Mycobacterium tuberculosis clinical isolates. J Clin Microbiol. 1995 May;33(5):1383–1384. doi: 10.1128/jcm.33.5.1383-1384.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Haas W. H., Butler W. R., Woodley C. L., Crawford J. T. Mixed-linker polymerase chain reaction: a new method for rapid fingerprinting of isolates of the Mycobacterium tuberculosis complex. J Clin Microbiol. 1993 May;31(5):1293–1298. doi: 10.1128/jcm.31.5.1293-1298.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hermans P. W., van Soolingen D., Bik E. M., de Haas P. E., Dale J. W., van Embden J. D. Insertion element IS987 from Mycobacterium bovis BCG is located in a hot-spot integration region for insertion elements in Mycobacterium tuberculosis complex strains. Infect Immun. 1991 Aug;59(8):2695–2705. doi: 10.1128/iai.59.8.2695-2705.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hermans P. W., van Soolingen D., Dale J. W., Schuitema A. R., McAdam R. A., Catty D., van Embden J. D. Insertion element IS986 from Mycobacterium tuberculosis: a useful tool for diagnosis and epidemiology of tuberculosis. J Clin Microbiol. 1990 Sep;28(9):2051–2058. doi: 10.1128/jcm.28.9.2051-2058.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mazurek G. H., Reddy V., Marston B. J., Haas W. H., Crawford J. T. DNA fingerprinting by infrequent-restriction-site amplification. J Clin Microbiol. 1996 Oct;34(10):2386–2390. doi: 10.1128/jcm.34.10.2386-2390.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Otal I., Samper S., Asensio M. P., Vitoria M. A., Rubio M. C., Gómez-Lus R., Martín C. Use of a PCR method based on IS6110 polymorphism for typing Mycobacterium tuberculosis strains from BACTEC cultures. J Clin Microbiol. 1997 Jan;35(1):273–277. doi: 10.1128/jcm.35.1.273-277.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Palittapongarnpim P., Chomyc S., Fanning A., Kunimoto D. DNA fingerprinting of Mycobacterium tuberculosis isolates by ligation-mediated polymerase chain reaction. Nucleic Acids Res. 1993 Feb 11;21(3):761–762. doi: 10.1093/nar/21.3.761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Patel S., Wall S., Saunders N. A. Heminested inverse PCR for IS6110 fingerprinting of Mycobacterium tuberculosis strains. J Clin Microbiol. 1996 Jul;34(7):1686–1690. doi: 10.1128/jcm.34.7.1686-1690.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Plikaytis B. B., Crawford J. T., Woodley C. L., Butler W. R., Eisenach K. D., Cave M. D., Shinnick T. M. Rapid, amplification-based fingerprinting of Mycobacterium tuberculosis. J Gen Microbiol. 1993 Jul;139(7):1537–1542. doi: 10.1099/00221287-139-7-1537. [DOI] [PubMed] [Google Scholar]
  13. Ross B. C., Dwyer B. Rapid, simple method for typing isolates of Mycobacterium tuberculosis by using the polymerase chain reaction. J Clin Microbiol. 1993 Feb;31(2):329–334. doi: 10.1128/jcm.31.2.329-334.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Torrea G., Offredo C., Simonet M., Gicquel B., Berche P., Pierre-Audigier C. Evaluation of tuberculosis transmission in a community by 1 year of systematic typing of Mycobacterium tuberculosis clinical isolates. J Clin Microbiol. 1996 May;34(5):1043–1049. doi: 10.1128/jcm.34.5.1043-1049.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Warren R., Richardson M., Sampson S., Hauman J. H., Beyers N., Donald P. R., van Helden P. D. Genotyping of Mycobacterium tuberculosis with additional markers enhances accuracy in epidemiological studies. J Clin Microbiol. 1996 Sep;34(9):2219–2224. doi: 10.1128/jcm.34.9.2219-2224.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yang Z., Chaves F., Barnes P. F., Burman W. J., Koehler J., Eisenach K. D., Bates J. H., Cave M. D. Evaluation of method for secondary DNA typing of Mycobacterium tuberculosis with pTBN12 in epidemiologic study of tuberculosis. J Clin Microbiol. 1996 Dec;34(12):3044–3048. doi: 10.1128/jcm.34.12.3044-3048.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. van Embden J. D., Cave M. D., Crawford J. T., Dale J. W., Eisenach K. D., Gicquel B., Hermans P., Martin C., McAdam R., Shinnick T. M. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol. 1993 Feb;31(2):406–409. doi: 10.1128/jcm.31.2.406-409.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

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