Abstract
Soluble forms of decay-accelerating factor (DAF) (CD55), the receptor for echovirus 7, were synthesized in the yeast Pichia pastoris. Purified recombinant protein containing SCR domains 2, 3, and 4, but lacking the serine/threonine rich region, was shown to block infection of susceptible cells by echovirus 7. In contrast to the situation with poliovirus and its receptor, the neutralization of echovirus 7 by soluble DAF was completely reversible and did not lead to the formation of 135S A-particles. Binding of virus to susceptible cells, by contrast, did lead to the formation of A particles, mainly from virus that had been internalized. The data suggest that a secondary factor(s) may contribute to A-particle formation and uncoating of echovirus 7.
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- Alkhatib G., Combadiere C., Broder C. C., Feng Y., Kennedy P. E., Murphy P. M., Berger E. A. CC CKR5: a RANTES, MIP-1alpha, MIP-1beta receptor as a fusion cofactor for macrophage-tropic HIV-1. Science. 1996 Jun 28;272(5270):1955–1958. doi: 10.1126/science.272.5270.1955. [DOI] [PubMed] [Google Scholar]
- Bergelson J. M., Chan M., Solomon K. R., St John N. F., Lin H., Finberg R. W. Decay-accelerating factor (CD55), a glycosylphosphatidylinositol-anchored complement regulatory protein, is a receptor for several echoviruses. Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):6245–6248. doi: 10.1073/pnas.91.13.6245. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bergelson J. M., Shepley M. P., Chan B. M., Hemler M. E., Finberg R. W. Identification of the integrin VLA-2 as a receptor for echovirus 1. Science. 1992 Mar 27;255(5052):1718–1720. doi: 10.1126/science.1553561. [DOI] [PubMed] [Google Scholar]
- Bernet-Camard M. F., Coconnier M. H., Hudault S., Servin A. L. Pathogenicity of the diffusely adhering strain Escherichia coli C1845: F1845 adhesin-decay accelerating factor interaction, brush border microvillus injury, and actin disassembly in cultured human intestinal epithelial cells. Infect Immun. 1996 Jun;64(6):1918–1928. doi: 10.1128/iai.64.6.1918-1928.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chan V. F., Black F. L. Uncoating of poliovirus by isolated plasma membranes. J Virol. 1970 Mar;5(3):309–312. doi: 10.1128/jvi.5.3.309-312.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clarkson N. A., Kaufman R., Lublin D. M., Ward T., Pipkin P. A., Minor P. D., Evans D. J., Almond J. W. Characterization of the echovirus 7 receptor: domains of CD55 critical for virus binding. J Virol. 1995 Sep;69(9):5497–5501. doi: 10.1128/jvi.69.9.5497-5501.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coyne K. E., Hall S. E., Thompson S., Arce M. A., Kinoshita T., Fujita T., Anstee D. J., Rosse W., Lublin D. M. Mapping of epitopes, glycosylation sites, and complement regulatory domains in human decay accelerating factor. J Immunol. 1992 Nov 1;149(9):2906–2913. [PubMed] [Google Scholar]
- Cregg J. M., Vedvick T. S., Raschke W. C. Recent advances in the expression of foreign genes in Pichia pastoris. Biotechnology (N Y) 1993 Aug;11(8):905–910. doi: 10.1038/nbt0893-905. [DOI] [PubMed] [Google Scholar]
- Crowell R. L., Philipson L. Specific alterations of coxsackievirus B3 eluted from HeLa cells. J Virol. 1971 Oct;8(4):509–515. doi: 10.1128/jvi.8.4.509-515.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Curry S., Chow M., Hogle J. M. The poliovirus 135S particle is infectious. J Virol. 1996 Oct;70(10):7125–7131. doi: 10.1128/jvi.70.10.7125-7131.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davitz M. A., Low M. G., Nussenzweig V. Release of decay-accelerating factor (DAF) from the cell membrane by phosphatidylinositol-specific phospholipase C (PIPLC). Selective modification of a complement regulatory protein. J Exp Med. 1986 May 1;163(5):1150–1161. doi: 10.1084/jem.163.5.1150. [DOI] [PMC free article] [PubMed] [Google Scholar]
- De Sena J., Mandel B. Studies on the in vitro uncoating of poliovirus. II. Characteristics of the membrane-modified particle. Virology. 1977 May 15;78(2):554–566. doi: 10.1016/0042-6822(77)90130-1. [DOI] [PubMed] [Google Scholar]
- Dragic T., Litwin V., Allaway G. P., Martin S. R., Huang Y., Nagashima K. A., Cayanan C., Maddon P. J., Koup R. A., Moore J. P. HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5. Nature. 1996 Jun 20;381(6584):667–673. doi: 10.1038/381667a0. [DOI] [PubMed] [Google Scholar]
- FENWICK M. L., COOPER P. D. Early interactions between poliovirus and ERK cells: some observations on the nature and significance of the rejected particles. Virology. 1962 Oct;18:212–223. doi: 10.1016/0042-6822(62)90007-7. [DOI] [PubMed] [Google Scholar]
- Fingeroth J. D., Weis J. J., Tedder T. F., Strominger J. L., Biro P. A., Fearon D. T. Epstein-Barr virus receptor of human B lymphocytes is the C3d receptor CR2. Proc Natl Acad Sci U S A. 1984 Jul;81(14):4510–4514. doi: 10.1073/pnas.81.14.4510. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fricks C. E., Hogle J. M. Cell-induced conformational change in poliovirus: externalization of the amino terminus of VP1 is responsible for liposome binding. J Virol. 1990 May;64(5):1934–1945. doi: 10.1128/jvi.64.5.1934-1945.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Greve J. M., Forte C. P., Marlor C. W., Meyer A. M., Hoover-Litty H., Wunderlich D., McClelland A. Mechanisms of receptor-mediated rhinovirus neutralization defined by two soluble forms of ICAM-1. J Virol. 1991 Nov;65(11):6015–6023. doi: 10.1128/jvi.65.11.6015-6023.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Guttman N., Baltimore D. A plasma membrane component able to bind and alter virions of poliovirus type 1: studies on cell-free alteration using a simplified assay. Virology. 1977 Oct 1;82(1):25–36. doi: 10.1016/0042-6822(77)90029-0. [DOI] [PubMed] [Google Scholar]
- Gómez Yafal A., Kaplan G., Racaniello V. R., Hogle J. M. Characterization of poliovirus conformational alteration mediated by soluble cell receptors. Virology. 1993 Nov;197(1):501–505. doi: 10.1006/viro.1993.1621. [DOI] [PubMed] [Google Scholar]
- HOLLAND J. J. Irreversible eclipse of poliovirus by HeLa cells. Virology. 1962 Feb;16:163–176. doi: 10.1016/0042-6822(62)90292-1. [DOI] [PubMed] [Google Scholar]
- Hamann J., Vogel B., van Schijndel G. M., van Lier R. A. The seven-span transmembrane receptor CD97 has a cellular ligand (CD55, DAF). J Exp Med. 1996 Sep 1;184(3):1185–1189. doi: 10.1084/jem.184.3.1185. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JOKLIK W. K., DARNELL J. E., Jr The adsorption and early fate of purified poliovirus in HeLa cells. Virology. 1961 Apr;13:439–447. doi: 10.1016/0042-6822(61)90275-6. [DOI] [PubMed] [Google Scholar]
- Kaplan G., Freistadt M. S., Racaniello V. R. Neutralization of poliovirus by cell receptors expressed in insect cells. J Virol. 1990 Oct;64(10):4697–4702. doi: 10.1128/jvi.64.10.4697-4702.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lonberg-Holm K., Whiteley N. M. Physical and metabolic requirements for early interaction of poliovirus and human rhinovirus with HeLa cells. J Virol. 1976 Sep;19(3):857–870. doi: 10.1128/jvi.19.3.857-870.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lublin D. M., Atkinson J. P. Decay-accelerating factor: biochemistry, molecular biology, and function. Annu Rev Immunol. 1989;7:35–58. doi: 10.1146/annurev.iy.07.040189.000343. [DOI] [PubMed] [Google Scholar]
- Manchester M., Liszewski M. K., Atkinson J. P., Oldstone M. B. Multiple isoforms of CD46 (membrane cofactor protein) serve as receptors for measles virus. Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2161–2165. doi: 10.1073/pnas.91.6.2161. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Naniche D., Varior-Krishnan G., Cervoni F., Wild T. F., Rossi B., Rabourdin-Combe C., Gerlier D. Human membrane cofactor protein (CD46) acts as a cellular receptor for measles virus. J Virol. 1993 Oct;67(10):6025–6032. doi: 10.1128/jvi.67.10.6025-6032.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Noble-Harvey J., Lonberg-Holm K. Sequential steps in attachment of human rhinovirus type 2 to HeLa cells. J Gen Virol. 1974 Oct;25(1):83–91. doi: 10.1099/0022-1317-25-1-83. [DOI] [PubMed] [Google Scholar]
- PHILIPSON L., BENGTSSON S., BRISHAMMAR S., SVENNERHOLM L., ZETTERQVIST O. PURIFICATION AND CHEMICAL ANALYSIS OF THE ERYTHROCYTE RECEPTOR FOR HEMAGGLUTINATING ENTEROVIRUSES. Virology. 1964 Apr;22:580–590. doi: 10.1016/0042-6822(64)90080-7. [DOI] [PubMed] [Google Scholar]
- Racaniello V. R. The poliovirus receptor: a hook, or an unzipper? Structure. 1996 Jul 15;4(7):769–773. doi: 10.1016/s0969-2126(96)00083-4. [DOI] [PubMed] [Google Scholar]
- Roesing T. G., Toselli P. A., Crowell R. L. Elution and uncoating of Coxsackievirus B3 by isolated HeLa cell plasma membranes. J Virol. 1975 Mar;15(3):654–657. doi: 10.1128/jvi.15.3.654-657.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shafren D. R., Dorahy D. J., Ingham R. A., Burns G. F., Barry R. D. Coxsackievirus A21 binds to decay-accelerating factor but requires intercellular adhesion molecule 1 for cell entry. J Virol. 1997 Jun;71(6):4736–4743. doi: 10.1128/jvi.71.6.4736-4743.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ward T., Pipkin P. A., Clarkson N. A., Stone D. M., Minor P. D., Almond J. W. Decay-accelerating factor CD55 is identified as the receptor for echovirus 7 using CELICS, a rapid immuno-focal cloning method. EMBO J. 1994 Nov 1;13(21):5070–5074. doi: 10.1002/j.1460-2075.1994.tb06836.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yousef G. E., Bell E. J., Mann G. F., Murugesan V., Smith D. G., McCartney R. A., Mowbray J. F. Chronic enterovirus infection in patients with postviral fatigue syndrome. Lancet. 1988 Jan 23;1(8578):146–150. doi: 10.1016/s0140-6736(88)92722-5. [DOI] [PubMed] [Google Scholar]