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. 1997 Dec;71(12):9600–9607. doi: 10.1128/jvi.71.12.9600-9607.1997

Activation of the adenovirus major late promoter by transcription factors MAZ and Sp1.

C L Parks 1, T Shenk 1
PMCID: PMC230268  PMID: 9371624

Abstract

Multiple binding sites for the transcription factors MAZ and Sp1 within the adenovirus type 5 major late promoter have been identified by DNase I protection studies. In the proximal region of the promoter, both MAZ and Sp1 interact with GC-rich sequences flanking the TATA box. Two MAZ binding sites are centered at -18 and -36 relative to the transcriptional initiation site. Sp1 bound only to the -18 GC-rich sequence. Several sites of interaction were also evident in the distal region of the promoter. Both MAZ and Sp1 interacted with a sequence centered at -166, and MAZ bound weakly to an additional site centered at -130. Overexpression of MAZ or Sp1 activated expression from the major late promoter in transient expression assays. Mutational analysis of the GC-rich sequences in the major late promoter suggested that a primary target of MAZ activation is the GC-rich sequences flanking the TATA sequence, whereas Sp1 requires the distal GC-rich sequence elements to stimulate gene expression. This activation is enhanced by the adenovirus E1A protein, and evidence for interaction between E1A and both transcription factors was obtained by using an immunoprecipitation assay. Activation by MAZ and Sp1 also was observed in transfection studies using the complete adenovirus type 5 genome as the target. Increased levels of late mRNA from both the L1 and L5 regions were observed when MAZ or Sp1 expression plasmids were transfected with viral DNA. Unexpectedly, activation of the major late promoter by MAZ and Sp1 was detected irrespective of whether the viral DNA could replicate.

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Selected References

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  1. Albrecht G., Devaux B., Kedinger C. Genomic footprinting detects factors bound to major late and IVa2 promoters in adenovirus-infected HeLa cells. Mol Cell Biol. 1988 Apr;8(4):1534–1539. doi: 10.1128/mcb.8.4.1534. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brunet L. J., Babiss L. E., Young C. S., Mills D. R. Mutations in the adenovirus major late promoter: effects on viability and transcription during infection. Mol Cell Biol. 1987 Mar;7(3):1091–1100. doi: 10.1128/mcb.7.3.1091. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  4. Geiger J. H., Hahn S., Lee S., Sigler P. B. Crystal structure of the yeast TFIIA/TBP/DNA complex. Science. 1996 May 10;272(5263):830–836. doi: 10.1126/science.272.5263.830. [DOI] [PubMed] [Google Scholar]
  5. Graham F. L., Smiley J., Russell W. C., Nairn R. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J Gen Virol. 1977 Jul;36(1):59–74. doi: 10.1099/0022-1317-36-1-59. [DOI] [PubMed] [Google Scholar]
  6. Hagen G., Müller S., Beato M., Suske G. Cloning by recognition site screening of two novel GT box binding proteins: a family of Sp1 related genes. Nucleic Acids Res. 1992 Nov 11;20(21):5519–5525. doi: 10.1093/nar/20.21.5519. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Harlow E., Franza B. R., Jr, Schley C. Monoclonal antibodies specific for adenovirus early region 1A proteins: extensive heterogeneity in early region 1A products. J Virol. 1985 Sep;55(3):533–546. doi: 10.1128/jvi.55.3.533-546.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Harlow E., Whyte P., Franza B. R., Jr, Schley C. Association of adenovirus early-region 1A proteins with cellular polypeptides. Mol Cell Biol. 1986 May;6(5):1579–1589. doi: 10.1128/mcb.6.5.1579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hyde-DeRuyscher R. P., Jennings E., Shenk T. DNA binding sites for the transcriptional activator/repressor YY1. Nucleic Acids Res. 1995 Nov 11;23(21):4457–4465. doi: 10.1093/nar/23.21.4457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jones N., Shenk T. Isolation of deletion and substitution mutants of adenovirus type 5. Cell. 1978 Jan;13(1):181–188. doi: 10.1016/0092-8674(78)90148-4. [DOI] [PubMed] [Google Scholar]
  11. Kingsley C., Winoto A. Cloning of GT box-binding proteins: a novel Sp1 multigene family regulating T-cell receptor gene expression. Mol Cell Biol. 1992 Oct;12(10):4251–4261. doi: 10.1128/mcb.12.10.4251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kolodziej P. A., Young R. A. Epitope tagging and protein surveillance. Methods Enzymol. 1991;194:508–519. doi: 10.1016/0076-6879(91)94038-e. [DOI] [PubMed] [Google Scholar]
  13. Manley J. L., Fire A., Samuels M., Sharp P. A. In vitro transcription: whole-cell extract. Methods Enzymol. 1983;101:568–582. doi: 10.1016/0076-6879(83)01038-1. [DOI] [PubMed] [Google Scholar]
  14. Martin K. J., Lillie J. W., Green M. R. Evidence for interaction of different eukaryotic transcriptional activators with distinct cellular targets. Nature. 1990 Jul 12;346(6280):147–152. doi: 10.1038/346147a0. [DOI] [PubMed] [Google Scholar]
  15. Nevins J. R., Wilson M. C. Regulation of adenovirus-2 gene expression at the level of transcriptional termination and RNA processing. Nature. 1981 Mar 12;290(5802):113–118. doi: 10.1038/290113a0. [DOI] [PubMed] [Google Scholar]
  16. Nikolov D. B., Chen H., Halay E. D., Usheva A. A., Hisatake K., Lee D. K., Roeder R. G., Burley S. K. Crystal structure of a TFIIB-TBP-TATA-element ternary complex. Nature. 1995 Sep 14;377(6545):119–128. doi: 10.1038/377119a0. [DOI] [PubMed] [Google Scholar]
  17. Parks C. L., Chang L. S., Shenk T. A polymerase chain reaction mediated by a single primer: cloning of genomic sequences adjacent to a serotonin receptor protein coding region. Nucleic Acids Res. 1991 Dec;19(25):7155–7160. doi: 10.1093/nar/19.25.7155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Parks C. L., Shenk T. The serotonin 1a receptor gene contains a TATA-less promoter that responds to MAZ and Sp1. J Biol Chem. 1996 Feb 23;271(8):4417–4430. doi: 10.1074/jbc.271.8.4417. [DOI] [PubMed] [Google Scholar]
  19. Reach M., Xu L. X., Young C. S. Transcription from the adenovirus major late promoter uses redundant activating elements. EMBO J. 1991 Nov;10(11):3439–3446. doi: 10.1002/j.1460-2075.1991.tb04908.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shi Y., Seto E., Chang L. S., Shenk T. Transcriptional repression by YY1, a human GLI-Krüppel-related protein, and relief of repression by adenovirus E1A protein. Cell. 1991 Oct 18;67(2):377–388. doi: 10.1016/0092-8674(91)90189-6. [DOI] [PubMed] [Google Scholar]
  21. Song B., Hu S. L., Darai G., Spindler K. R., Young C. S. Conservation of DNA sequence in the predicted major late promoter regions of selected mastadenoviruses. Virology. 1996 Jun 15;220(2):390–401. doi: 10.1006/viro.1996.0327. [DOI] [PubMed] [Google Scholar]
  22. Tan S., Hunziker Y., Sargent D. F., Richmond T. J. Crystal structure of a yeast TFIIA/TBP/DNA complex. Nature. 1996 May 9;381(6578):127–151. doi: 10.1038/381127a0. [DOI] [PubMed] [Google Scholar]
  23. Thomas G. P., Mathews M. B. DNA replication and the early to late transition in adenovirus infection. Cell. 1980 Nov;22(2 Pt 2):523–533. doi: 10.1016/0092-8674(80)90362-1. [DOI] [PubMed] [Google Scholar]
  24. Volkert F. C., Young C. S. The genetic analysis of recombination using adenovirus overlapping terminal DNA fragments. Virology. 1983 Feb;125(1):175–193. doi: 10.1016/0042-6822(83)90072-7. [DOI] [PubMed] [Google Scholar]
  25. Yu Y. T., Manley J. L. Generation and functional analyses for base-substitution mutants of the adenovirus 2 major late promoter. Nucleic Acids Res. 1984 Dec 21;12(24):9309–9321. doi: 10.1093/nar/12.24.9309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Yu Y. T., Manley J. L. Structure and function of the S1 nuclease-sensitive site in the adenovirus late promoter. Cell. 1986 Jun 6;45(5):743–751. doi: 10.1016/0092-8674(86)90788-9. [DOI] [PubMed] [Google Scholar]

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