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. 1995 Aug;15(8):3998–4008. doi: 10.1128/mcb.15.8.3998

The essential helicase gene RAD3 suppresses short-sequence recombination in Saccharomyces cerevisiae.

A M Bailis 1, S Maines 1, M T Negritto 1
PMCID: PMC230639  PMID: 7623796

Abstract

We have isolated an allele of the essential DNA repair and transcription gene RAD3 that relaxes the restriction against recombination between short DNA sequences in Saccharomyces cerevisiae. Double-strand break repair and gene replacement events requiring recombination between short identical or mismatched sequences were stimulated in the rad3-G595R mutant cells. We also observed an increase in the physical stability of double-strand breaks in the rad3-G595R mutant cells. These results suggest that the RAD3 gene suppresses recombination involving short homologous sequences by promoting the degradation of the ends of broken DNA molecules.

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Selected References

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