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. 1995 Sep;15(9):5188–5195. doi: 10.1128/mcb.15.9.5188

The N-terminal domain of c-Myc associates with alpha-tubulin and microtubules in vivo and in vitro.

N Alexandrova 1, J Niklinski 1, V Bliskovsky 1, G A Otterson 1, M Blake 1, F J Kaye 1, M Zajac-Kaye 1
PMCID: PMC230766  PMID: 7651436

Abstract

The polymerization of alpha- and beta-tubulin into microtubules results in a complex network of microfibrils that have important structural and functional roles in all eukaryotic cells. In addition, microtubules can interact with a diverse family of polypeptides which are believed to directly promote the assembly of microtubules and to modulate their functional activity. We have demonstrated that the c-Myc oncoprotein interacts in vivo and in vitro with alpha-tubulin and with polymerized microtubules and have defined the binding site to the N-terminal region within the transactivation domain of c-Myc. In addition, we have shown that c-Myc colocalizes with microtubules and remains tightly bound to the microtubule network after detergent extraction of intact cells. These findings suggest a potential role for Myc-tubulin interaction in vivo.

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Selected References

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