Fig. 6.

Reduction of EGFR tyrosine kinase activity inhibits the H₂O₂-induced nuclear translocation of activated PKA. C10 cells, pre-incubated with 10 μM AG1478 for 1 h, were exposed to 10 μM forskolin and H₂O₂ for the indicated concentrations for 10 min. (a) The PKA free catalytic subunit (red) was detected by immunofluorescence and nuclei were stained with YOYO-1 (green). (b) Quantification of PKA immunofluorescence pixel intensity within nuclei; *P < 0.05 compared to control, ^#P < 0.05 compared to treatment, n = 3 experiments, 5 fields/experiment