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. 1996 Jun;16(6):2700–2707. doi: 10.1128/mcb.16.6.2700

The newly identified yeast GRD genes are required for retention of late-Golgi membrane proteins.

S F Nothwehr 1, N J Bryant 1, T H Stevens 1
PMCID: PMC231260  PMID: 8649377

Abstract

Processing of A-ALP, a late-Golgi membrane protein constructed by fusing the cytosolic domain of dipeptidyl aminopeptidase A to the transmembrane and lumenal domains of alkaline phosphatase (ALP), serves as a convenient assay for loss of retention of late-Golgi membrane proteins in Saccharomyces cerevisiae. In this study, a large group of novel grd (for Golgi retention defective) yeast mutants, representing 18 complementation groups, were identified on the basis of their mislocalization of A-ALP to the vacuole, where it was proteolytically processed and thus became enzymatically activated. All of the grd mutants exhibited significant mislocalization of A-ALP, as measured by determining the kinetics of A-ALP processing and by analyzing its

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Selected References

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