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. 1996 Jul;16(7):3401–3409. doi: 10.1128/mcb.16.7.3401

Cell cycle regulation of the murine cyclin E gene depends on an E2F binding site in the promoter.

J Botz 1, K Zerfass-Thome 1, D Spitkovsky 1, H Delius 1, B Vogt 1, M Eilers 1, A Hatzigeorgiou 1, P Jansen-Dürr 1
PMCID: PMC231334  PMID: 8668155

Abstract

Cyclin E controls progression through the G1 phase of the cell cycle in mammalian fibroblasts and potentially in many other cell types. Cyclin E is a rate-limiting activator of cdk2 kinase in late G1. The abundance of cyclin E is controlled by phase-specific fluctuations in the mRNA level; in mammalian fibroblasts, mRNA is not detected under conditions of serum starvation and is accumulated upon serum stimulation, with expression starting in mid-G1. Here, we report the cloning of the murine cyclin E promoter. We isolated a 3.8-kb genomic fragment that contains several transcriptional start sites and confers cell cycle regulation on a luciferase reporter gene. This fragment also supports transcriptional activation by adenovirus E1A, a known upstream regulator of cyclin E gene expression. An E2F binding site which is required for G1-specific activation of the cyclin E promoter in synchronized NIH 3T3 cells was identified in this fragment.

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Selected References

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