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Self-antigen arrests the in vitro maturation of 3–83Tg B cells as revealed by three-color flow cytometry analysis of cell surface markers. B cell precursors from 3–83Tg mice were grown for 5 days on antigen-free (S17) or antigen-bearing (op42) stromal cell lines in the presence of IL-7 and stained for the expression of surface IgM, the pan B cell marker B220, and the indicated cell surface markers. Results shown were gated on the B220+ population to exclude stromal cell contamination.
