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. 1997 Mar;17(3):1346–1353. doi: 10.1128/mcb.17.3.1346

Lck regulates Vav activation of members of the Rho family of GTPases.

J Han 1, B Das 1, W Wei 1, L Van Aelst 1, R D Mosteller 1, R Khosravi-Far 1, J K Westwick 1, C J Der 1, D Broek 1
PMCID: PMC231859  PMID: 9032261

Abstract

Vav is a member of a family of oncogene proteins that share an approximately 250-amino-acid motif called a Dbl homology domain. Paradoxically, Dbl itself and other proteins containing a Dbl domain catalyze GTP-GDP exchange for Rho family proteins, whereas Vav has been reported to catalyze GTP-GDP exchange for Ras proteins. We present Saccharomyces cerevisiae genetic data, in vitro biochemical data, and animal cell biological data indicating that Vav is a guanine nucleotide exchange factor for Rho-related proteins, but in similar genetic and biochemical experiments we fail to find evidence that Vav is a guanine nucleotide exchange factor for Ras. Further, we present data indicating that the Lck kinase activates the guanine nucleotide exchange factor and transforming activity of Vav.

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Selected References

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