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. 1995 Feb;15(2):1005–1013. doi: 10.1128/mcb.15.2.1005

Hormone-independent repression of AP-1-inducible collagenase promoter activity by glucocorticoid receptors.

W Liu 1, A G Hillmann 1, J M Harmon 1
PMCID: PMC231996  PMID: 7823916

Abstract

The role of the ligand in glucocorticoid receptor-mediated transactivation and transrepression of gene expression was investigated. Half-maximal transactivation of a mouse mammary tumor virus-chloramphenicol acetyltransferase reporter gene in transfected cells expressing the human glucocorticoid receptor mutant GRL753F, from which the rate of ligand dissociation is four to five times higher than the rate of dissociation from normal receptors, required a 200- to 300-fold-higher concentration of dexamethasone than was required in cells expressing the normal receptor. Immunocytochemical analysis demonstrated that this difference was not the result of a failure of the mutant receptor to accumulate in the nucleus after steroid treatment. In contrast, in cells cotransfected with a reporter gene containing the AP-1-inducible collagenase gene promoter, the concentration of dexamethasone required for 50% transrepression was the same for mutant and normal receptors. Efficient receptor-mediated transrepression was also observed with the double mutant GRL753F/C421Y, in which the first cysteine residue of the proximal zinc finger has been replaced by tyrosine, indicating that neither retention of the ligand nor direct binding of the receptor to DNA is required. RU38486 behaved as a full agonist with respect to transrepression. In addition, receptor-dependent transrepression, but not transactivation, was observed in transfected cells after heat shock in the absence of the ligand. Taken together, these results suggest that unlike transactivation, transrepression of AP-1 activity by the nuclear glucocorticoid receptor is ligand independent.

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Selected References

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  1. Akerblom I. E., Slater E. P., Beato M., Baxter J. D., Mellon P. L. Negative regulation by glucocorticoids through interference with a cAMP responsive enhancer. Science. 1988 Jul 15;241(4863):350–353. doi: 10.1126/science.2838908. [DOI] [PubMed] [Google Scholar]
  2. Allan G. F., Tsai S. Y., Tsai M. J., O'Malley B. W. Ligand-dependent conformational changes in the progesterone receptor are necessary for events that follow DNA binding. Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11750–11754. doi: 10.1073/pnas.89.24.11750. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ashraf J., Thompson E. B. Identification of the activation-labile gene: a single point mutation in the human glucocorticoid receptor presents as two distinct receptor phenotypes. Mol Endocrinol. 1993 May;7(5):631–642. doi: 10.1210/mend.7.5.8316249. [DOI] [PubMed] [Google Scholar]
  4. Beato M. Transcriptional control by nuclear receptors. FASEB J. 1991 Apr;5(7):2044–2051. doi: 10.1096/fasebj.5.7.2010057. [DOI] [PubMed] [Google Scholar]
  5. Beekman J. M., Allan G. F., Tsai S. Y., Tsai M. J., O'Malley B. W. Transcriptional activation by the estrogen receptor requires a conformational change in the ligand binding domain. Mol Endocrinol. 1993 Oct;7(10):1266–1274. doi: 10.1210/mend.7.10.8264659. [DOI] [PubMed] [Google Scholar]
  6. Borrás T., Peterson C. A., Piatigorsky J. Evidence for positive and negative regulation in the promoter of the chicken delta 1-crystallin gene. Dev Biol. 1988 May;127(1):209–219. doi: 10.1016/0012-1606(88)90202-3. [DOI] [PubMed] [Google Scholar]
  7. Burnstein K. L., Jewell C. M., Cidlowski J. A. Human glucocorticoid receptor cDNA contains sequences sufficient for receptor down-regulation. J Biol Chem. 1990 May 5;265(13):7284–7291. [PubMed] [Google Scholar]
  8. Cairns C., Cairns W., Okret S. Inhibition of gene expression by steroid hormone receptors via a negative glucocorticoid response element: evidence for the involvement of DNA-binding and agonistic effects of the antiglucocorticoid/antiprogestin RU486. DNA Cell Biol. 1993 Oct;12(8):695–702. doi: 10.1089/dna.1993.12.695. [DOI] [PubMed] [Google Scholar]
  9. Celada A., McKercher S., Maki R. A. Repression of major histocompatibility complex IA expression by glucocorticoids: the glucocorticoid receptor inhibits the DNA binding of the X box DNA binding protein. J Exp Med. 1993 Mar 1;177(3):691–698. doi: 10.1084/jem.177.3.691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Chakraborti P. K., Simons S. S., Jr Association of heat shock protein 90 with the 16 kDa steroid binding core fragment of rat glucocorticoid receptors. Biochem Biophys Res Commun. 1991 May 15;176(3):1338–1344. doi: 10.1016/0006-291x(91)90433-8. [DOI] [PubMed] [Google Scholar]
  11. Chen C., Okayama H. High-efficiency transformation of mammalian cells by plasmid DNA. Mol Cell Biol. 1987 Aug;7(8):2745–2752. doi: 10.1128/mcb.7.8.2745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Chen D., Kohli K., Zhang S., Danielsen M., Stallcup M. R. Phenylalanine-780 near the C-terminus of the mouse glucocorticoid receptor is important for ligand binding affinity and specificity. Mol Endocrinol. 1994 Apr;8(4):422–430. doi: 10.1210/mend.8.4.8052263. [DOI] [PubMed] [Google Scholar]
  13. Chen D., Stallcup M. R. The hormone-binding role of 2 cysteines near the C terminus of the mouse glucocorticoid receptor. J Biol Chem. 1994 Mar 18;269(11):7914–7918. [PubMed] [Google Scholar]
  14. Dalman F. C., Scherrer L. C., Taylor L. P., Akil H., Pratt W. B. Localization of the 90-kDa heat shock protein-binding site within the hormone-binding domain of the glucocorticoid receptor by peptide competition. J Biol Chem. 1991 Feb 25;266(6):3482–3490. [PubMed] [Google Scholar]
  15. Danielian P. S., White R., Lees J. A., Parker M. G. Identification of a conserved region required for hormone dependent transcriptional activation by steroid hormone receptors. EMBO J. 1992 Mar;11(3):1025–1033. doi: 10.1002/j.1460-2075.1992.tb05141.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Danielsen M., Northrop J. P., Ringold G. M. The mouse glucocorticoid receptor: mapping of functional domains by cloning, sequencing and expression of wild-type and mutant receptor proteins. EMBO J. 1986 Oct;5(10):2513–2522. doi: 10.1002/j.1460-2075.1986.tb04529.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Denis M., Poellinger L., Wikstöm A. C., Gustafsson J. A. Requirement of hormone for thermal conversion of the glucocorticoid receptor to a DNA-binding state. Nature. 1988 Jun 16;333(6174):686–688. doi: 10.1038/333686a0. [DOI] [PubMed] [Google Scholar]
  18. Denis M., Wikström A. C., Gustafsson J. A. The molybdate-stabilized nonactivated glucocorticoid receptor contains a dimer of Mr 90,000 non-hormone-binding protein. J Biol Chem. 1987 Aug 25;262(24):11803–11806. [PubMed] [Google Scholar]
  19. Denner L. A., Weigel N. L., Maxwell B. L., Schrader W. T., O'Malley B. W. Regulation of progesterone receptor-mediated transcription by phosphorylation. Science. 1990 Dec 21;250(4988):1740–1743. doi: 10.1126/science.2176746. [DOI] [PubMed] [Google Scholar]
  20. Diamond M. I., Miner J. N., Yoshinaga S. K., Yamamoto K. R. Transcription factor interactions: selectors of positive or negative regulation from a single DNA element. Science. 1990 Sep 14;249(4974):1266–1272. doi: 10.1126/science.2119054. [DOI] [PubMed] [Google Scholar]
  21. Drouin J., Trifiro M. A., Plante R. K., Nemer M., Eriksson P., Wrange O. Glucocorticoid receptor binding to a specific DNA sequence is required for hormone-dependent repression of pro-opiomelanocortin gene transcription. Mol Cell Biol. 1989 Dec;9(12):5305–5314. doi: 10.1128/mcb.9.12.5305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Elsasser M. S., Eisen L. P., Riegel A. T., Harmon J. M. Stability and sequence-specific DNA binding of activation-labile mutants of the human glucocorticoid receptor. Biochemistry. 1991 Nov 19;30(46):11140–11146. doi: 10.1021/bi00110a017. [DOI] [PubMed] [Google Scholar]
  23. Fawell S. E., Lees J. A., White R., Parker M. G. Characterization and colocalization of steroid binding and dimerization activities in the mouse estrogen receptor. Cell. 1990 Mar 23;60(6):953–962. doi: 10.1016/0092-8674(90)90343-d. [DOI] [PubMed] [Google Scholar]
  24. Gauthier J. M., Bourachot B., Doucas V., Yaniv M., Moreau-Gachelin F. Functional interference between the Spi-1/PU.1 oncoprotein and steroid hormone or vitamin receptors. EMBO J. 1993 Dec 15;12(13):5089–5096. doi: 10.1002/j.1460-2075.1993.tb06203.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Giguère V., Hollenberg S. M., Rosenfeld M. G., Evans R. M. Functional domains of the human glucocorticoid receptor. Cell. 1986 Aug 29;46(5):645–652. doi: 10.1016/0092-8674(86)90339-9. [DOI] [PubMed] [Google Scholar]
  26. Gorman C. M., Merlino G. T., Willingham M. C., Pastan I., Howard B. H. The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6777–6781. doi: 10.1073/pnas.79.22.6777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Groyer A., Schweizer-Groyer G., Cadepond F., Mariller M., Baulieu E. E. Antiglucocorticosteroid effects suggest why steroid hormone is required for receptors to bind DNA in vivo but not in vitro. Nature. 1987 Aug 13;328(6131):624–626. doi: 10.1038/328624a0. [DOI] [PubMed] [Google Scholar]
  28. Gruol D. J., Wolfe K. A. Transformation of glucocorticoid receptors bound to the antagonist RU 486: effects of alkaline phosphatase. Biochemistry. 1990 Aug 28;29(34):7958–7966. doi: 10.1021/bi00486a026. [DOI] [PubMed] [Google Scholar]
  29. Harmon J. M., Elsasser M. S., Eisen L. P., Urda L. A., Ashraf J., Thompson E. B. Glucocorticoid receptor expression in receptorless mutants isolated from the human leukemic cell line CEM-C7. Mol Endocrinol. 1989 Apr;3(4):734–743. doi: 10.1210/mend-3-4-734. [DOI] [PubMed] [Google Scholar]
  30. Harmon J. M., Norman M. R., Fowlkes B. J., Thompson E. B. Dexamethasone induces irreversible G1 arrest and death of a human lymphoid cell line. J Cell Physiol. 1979 Feb;98(2):267–278. doi: 10.1002/jcp.1040980203. [DOI] [PubMed] [Google Scholar]
  31. Harmon J. M., Thompson E. B. Isolation and characterization of dexamethasone-resistant mutants from human lymphoid cell line CEM-C7. Mol Cell Biol. 1981 Jun;1(6):512–521. doi: 10.1128/mcb.1.6.512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Heck S., Kullmann M., Gast A., Ponta H., Rahmsdorf H. J., Herrlich P., Cato A. C. A distinct modulating domain in glucocorticoid receptor monomers in the repression of activity of the transcription factor AP-1. EMBO J. 1994 Sep 1;13(17):4087–4095. doi: 10.1002/j.1460-2075.1994.tb06726.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Hoeck W., Rusconi S., Groner B. Down-regulation and phosphorylation of glucocorticoid receptors in cultured cells. Investigations with a monospecific antiserum against a bacterially expressed receptor fragment. J Biol Chem. 1989 Aug 25;264(24):14396–14402. [PubMed] [Google Scholar]
  34. Hollenberg S. M., Evans R. M. Multiple and cooperative trans-activation domains of the human glucocorticoid receptor. Cell. 1988 Dec 2;55(5):899–906. doi: 10.1016/0092-8674(88)90145-6. [DOI] [PubMed] [Google Scholar]
  35. Howard K. J., Distelhorst C. W. Evidence for intracellular association of the glucocorticoid receptor with the 90-kDa heat shock protein. J Biol Chem. 1988 Mar 5;263(7):3474–3481. [PubMed] [Google Scholar]
  36. Howard K. J., Holley S. J., Yamamoto K. R., Distelhorst C. W. Mapping the HSP90 binding region of the glucocorticoid receptor. J Biol Chem. 1990 Jul 15;265(20):11928–11935. [PubMed] [Google Scholar]
  37. Ince B. A., Zhuang Y., Wrenn C. K., Shapiro D. J., Katzenellenbogen B. S. Powerful dominant negative mutants of the human estrogen receptor. J Biol Chem. 1993 Jul 5;268(19):14026–14032. [PubMed] [Google Scholar]
  38. Jonat C., Rahmsdorf H. J., Park K. K., Cato A. C., Gebel S., Ponta H., Herrlich P. Antitumor promotion and antiinflammation: down-modulation of AP-1 (Fos/Jun) activity by glucocorticoid hormone. Cell. 1990 Sep 21;62(6):1189–1204. doi: 10.1016/0092-8674(90)90395-u. [DOI] [PubMed] [Google Scholar]
  39. Kerppola T. K., Luk D., Curran T. Fos is a preferential target of glucocorticoid receptor inhibition of AP-1 activity in vitro. Mol Cell Biol. 1993 Jun;13(6):3782–3791. doi: 10.1128/mcb.13.6.3782. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Kutoh E., Strömstedt P. E., Poellinger L. Functional interference between the ubiquitous and constitutive octamer transcription factor 1 (OTF-1) and the glucocorticoid receptor by direct protein-protein interaction involving the homeo subdomain of OTF-1. Mol Cell Biol. 1992 Nov;12(11):4960–4969. doi: 10.1128/mcb.12.11.4960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Lanz R. B., Hug M., Gola M., Tallone T., Wieland S., Rusconi S. Active, interactive, and inactive steroid receptor mutants. Steroids. 1994 Feb;59(2):148–152. doi: 10.1016/0039-128x(94)90093-0. [DOI] [PubMed] [Google Scholar]
  42. Lebeau M. C., Massol N., Herrick J., Faber L. E., Renoir J. M., Radanyi C., Baulieu E. E. P59, an hsp 90-binding protein. Cloning and sequencing of its cDNA and preparation of a peptide-directed polyclonal antibody. J Biol Chem. 1992 Mar 5;267(7):4281–4284. [PubMed] [Google Scholar]
  43. Lucibello F. C., Slater E. P., Jooss K. U., Beato M., Müller R. Mutual transrepression of Fos and the glucocorticoid receptor: involvement of a functional domain in Fos which is absent in FosB. EMBO J. 1990 Sep;9(9):2827–2834. doi: 10.1002/j.1460-2075.1990.tb07471.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. Malchoff D. M., Brufsky A., Reardon G., McDermott P., Javier E. C., Bergh C. H., Rowe D., Malchoff C. D. A mutation of the glucocorticoid receptor in primary cortisol resistance. J Clin Invest. 1993 May;91(5):1918–1925. doi: 10.1172/JCI116410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. Mendel D. B., Bodwell J. E., Gametchu B., Harrison R. W., Munck A. Molybdate-stabilized nonactivated glucocorticoid-receptor complexes contain a 90-kDa non-steroid-binding phosphoprotein that is lost on activation. J Biol Chem. 1986 Mar 15;261(8):3758–3763. [PubMed] [Google Scholar]
  46. Mordacq J. C., Linzer D. I. Co-localization of elements required for phorbol ester stimulation and glucocorticoid repression of proliferin gene expression. Genes Dev. 1989 Jun;3(6):760–769. doi: 10.1101/gad.3.6.760. [DOI] [PubMed] [Google Scholar]
  47. Nemoto T., Ohara-Nemoto Y., Denis M., Gustafsson J. A. The transformed glucocorticoid receptor has a lower steroid-binding affinity than the nontransformed receptor. Biochemistry. 1990 Feb 20;29(7):1880–1886. doi: 10.1021/bi00459a031. [DOI] [PubMed] [Google Scholar]
  48. Norman M. R., Thompson E. B. Characterization of a glucocorticoid-sensitive human lymphoid cell line. Cancer Res. 1977 Oct;37(10):3785–3791. [PubMed] [Google Scholar]
  49. Oro A. E., Hollenberg S. M., Evans R. M. Transcriptional inhibition by a glucocorticoid receptor-beta-galactosidase fusion protein. Cell. 1988 Dec 23;55(6):1109–1114. doi: 10.1016/0092-8674(88)90255-3. [DOI] [PubMed] [Google Scholar]
  50. Palmer L. A., Harmon J. M. Biochemical evidence that glucocorticoid-sensitive cell lines derived from the human leukemic cell line CCRF-CEM express a normal and a mutant glucocorticoid receptor gene. Cancer Res. 1991 Oct 1;51(19):5224–5231. [PubMed] [Google Scholar]
  51. Pfahl M. Nuclear receptor/AP-1 interaction. Endocr Rev. 1993 Oct;14(5):651–658. doi: 10.1210/edrv-14-5-651. [DOI] [PubMed] [Google Scholar]
  52. Picard D., Khursheed B., Garabedian M. J., Fortin M. G., Lindquist S., Yamamoto K. R. Reduced levels of hsp90 compromise steroid receptor action in vivo. Nature. 1990 Nov 8;348(6297):166–168. doi: 10.1038/348166a0. [DOI] [PubMed] [Google Scholar]
  53. Power R. F., Conneely O. M., O'Malley B. W. New insights into activation of the steroid hormone receptor superfamily. Trends Pharmacol Sci. 1992 Aug;13(8):318–323. doi: 10.1016/0165-6147(92)90099-r. [DOI] [PubMed] [Google Scholar]
  54. Power R. F., Lydon J. P., Conneely O. M., O'Malley B. W. Dopamine activation of an orphan of the steroid receptor superfamily. Science. 1991 Jun 14;252(5012):1546–1548. doi: 10.1126/science.2047861. [DOI] [PubMed] [Google Scholar]
  55. Power R. F., Mani S. K., Codina J., Conneely O. M., O'Malley B. W. Dopaminergic and ligand-independent activation of steroid hormone receptors. Science. 1991 Dec 13;254(5038):1636–1639. doi: 10.1126/science.1749936. [DOI] [PubMed] [Google Scholar]
  56. Powers J. H., Hillmann A. G., Tang D. C., Harmon J. M. Cloning and expression of mutant glucocorticoid receptors from glucocorticoid-sensitive and -resistant human leukemic cells. Cancer Res. 1993 Sep 1;53(17):4059–4065. [PubMed] [Google Scholar]
  57. Pratt W. B., Czar M. J., Stancato L. F., Owens J. K. The hsp56 immunophilin component of steroid receptor heterocomplexes: could this be the elusive nuclear localization signal-binding protein? J Steroid Biochem Mol Biol. 1993 Sep;46(3):269–279. doi: 10.1016/0960-0760(93)90216-j. [DOI] [PubMed] [Google Scholar]
  58. Pratt W. B., Jolly D. J., Pratt D. V., Hollenberg S. M., Giguere V., Cadepond F. M., Schweizer-Groyer G., Catelli M. G., Evans R. M., Baulieu E. E. A region in the steroid binding domain determines formation of the non-DNA-binding, 9 S glucocorticoid receptor complex. J Biol Chem. 1988 Jan 5;263(1):267–273. [PubMed] [Google Scholar]
  59. Qi M., Stasenko L. J., DeFranco D. B. Recycling and desensitization of glucocorticoid receptors in v-mos transformed cells depend on the ability of nuclear receptors to modulate gene expression. Mol Endocrinol. 1990 Mar;4(3):455–464. doi: 10.1210/mend-4-3-455. [DOI] [PubMed] [Google Scholar]
  60. Ray A., Prefontaine K. E. Physical association and functional antagonism between the p65 subunit of transcription factor NF-kappa B and the glucocorticoid receptor. Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):752–756. doi: 10.1073/pnas.91.2.752. [DOI] [PMC free article] [PubMed] [Google Scholar]
  61. Rexin M., Busch W., Segnitz B., Gehring U. Structure of the glucocorticoid receptor in intact cells in the absence of hormone. J Biol Chem. 1992 May 15;267(14):9619–9621. [PubMed] [Google Scholar]
  62. Sakai D. D., Helms S., Carlstedt-Duke J., Gustafsson J. A., Rottman F. M., Yamamoto K. R. Hormone-mediated repression: a negative glucocorticoid response element from the bovine prolactin gene. Genes Dev. 1988 Sep;2(9):1144–1154. doi: 10.1101/gad.2.9.1144. [DOI] [PubMed] [Google Scholar]
  63. Sanchez E. R. Heat shock induces translocation to the nucleus of the unliganded glucocorticoid receptor. J Biol Chem. 1992 Jan 5;267(1):17–20. [PubMed] [Google Scholar]
  64. Sanchez E. R. Hsp56: a novel heat shock protein associated with untransformed steroid receptor complexes. J Biol Chem. 1990 Dec 25;265(36):22067–22070. [PubMed] [Google Scholar]
  65. Sanchez E. R., Hu J. L., Zhong S., Shen P., Greene M. J., Housley P. R. Potentiation of glucocorticoid receptor-mediated gene expression by heat and chemical shock. Mol Endocrinol. 1994 Apr;8(4):408–421. doi: 10.1210/mend.8.4.8052262. [DOI] [PubMed] [Google Scholar]
  66. Sanchez E. R., Meshinchi S., Tienrungroj W., Schlesinger M. J., Toft D. O., Pratt W. B. Relationship of the 90-kDa murine heat shock protein to the untransformed and transformed states of the L cell glucocorticoid receptor. J Biol Chem. 1987 May 25;262(15):6986–6991. [PubMed] [Google Scholar]
  67. Sanchez E. R., Toft D. O., Schlesinger M. J., Pratt W. B. Evidence that the 90-kDa phosphoprotein associated with the untransformed L-cell glucocorticoid receptor is a murine heat shock protein. J Biol Chem. 1985 Oct 15;260(23):12398–12401. [PubMed] [Google Scholar]
  68. Schmidt T. J. Comparison of in vivo activation of triamcinolone acetonide- and RU 38486-receptor complexes in the CEM-C7 and IM-9 human leukemic cell lines. Cancer Res. 1989 Aug 15;49(16):4390–4395. [PubMed] [Google Scholar]
  69. Schüle R., Evans R. M. Cross-coupling of signal transduction pathways: zinc finger meets leucine zipper. Trends Genet. 1991 Nov-Dec;7(11-12):377–381. doi: 10.1016/0168-9525(91)90259-s. [DOI] [PubMed] [Google Scholar]
  70. Schüle R., Rangarajan P., Kliewer S., Ransone L. J., Bolado J., Yang N., Verma I. M., Evans R. M. Functional antagonism between oncoprotein c-Jun and the glucocorticoid receptor. Cell. 1990 Sep 21;62(6):1217–1226. doi: 10.1016/0092-8674(90)90397-w. [DOI] [PubMed] [Google Scholar]
  71. Seed B., Sheen J. Y. A simple phase-extraction assay for chloramphenicol acyltransferase activity. Gene. 1988 Jul 30;67(2):271–277. doi: 10.1016/0378-1119(88)90403-9. [DOI] [PubMed] [Google Scholar]
  72. Segnitz B., Gehring U. Mechanism of action of a steroidal antiglucocorticoid in lymphoid cells. J Biol Chem. 1990 Feb 15;265(5):2789–2796. [PubMed] [Google Scholar]
  73. Shen P., Xie Z. J., Li H., Sánchez E. R. Glucocorticoid receptor conversion to high affinity nuclear binding and transcription enhancement activity in Chinese hamster ovary cells subjected to heat and chemical stress. J Steroid Biochem Mol Biol. 1993 Dec;47(1-6):55–64. doi: 10.1016/0960-0760(93)90057-4. [DOI] [PubMed] [Google Scholar]
  74. Simons S. S., Jr, Sistare F. D., Chakraborti P. K. Steroid binding activity is retained in a 16-kDa fragment of the steroid binding domain of rat glucocorticoid receptors. J Biol Chem. 1989 Aug 25;264(24):14493–14497. [PubMed] [Google Scholar]
  75. Strömstedt P. E., Poellinger L., Gustafsson J. A., Carlstedt-Duke J. The glucocorticoid receptor binds to a sequence overlapping the TATA box of the human osteocalcin promoter: a potential mechanism for negative regulation. Mol Cell Biol. 1991 Jun;11(6):3379–3383. doi: 10.1128/mcb.11.6.3379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  76. Touray M., Ryan F., Jaggi R., Martin F. Characterisation of functional inhibition of the glucocorticoid receptor by Fos/Jun. Oncogene. 1991 Jul;6(7):1227–1234. [PubMed] [Google Scholar]
  77. Tverberg L. A., Russo A. F. Cell-specific glucocorticoid repression of calcitonin/calcitonin gene-related peptide transcription. Localization to an 18-base pair basal enhancer element. J Biol Chem. 1992 Sep 5;267(25):17567–17573. [PubMed] [Google Scholar]
  78. Webster N. J., Green S., Jin J. R., Chambon P. The hormone-binding domains of the estrogen and glucocorticoid receptors contain an inducible transcription activation function. Cell. 1988 Jul 15;54(2):199–207. doi: 10.1016/0092-8674(88)90552-1. [DOI] [PubMed] [Google Scholar]
  79. Willmann T., Beato M. Steroid-free glucocorticoid receptor binds specifically to mouse mammary tumour virus DNA. Nature. 1986 Dec 18;324(6098):688–691. doi: 10.1038/324688a0. [DOI] [PubMed] [Google Scholar]
  80. Yang-Yen H. F., Chambard J. C., Sun Y. L., Smeal T., Schmidt T. J., Drouin J., Karin M. Transcriptional interference between c-Jun and the glucocorticoid receptor: mutual inhibition of DNA binding due to direct protein-protein interaction. Cell. 1990 Sep 21;62(6):1205–1215. doi: 10.1016/0092-8674(90)90396-v. [DOI] [PubMed] [Google Scholar]
  81. Yem A. W., Tomasselli A. G., Heinrikson R. L., Zurcher-Neely H., Ruff V. A., Johnson R. A., Deibel M. R., Jr The Hsp56 component of steroid receptor complexes binds to immobilized FK506 and shows homology to FKBP-12 and FKBP-13. J Biol Chem. 1992 Feb 15;267(5):2868–2871. [PubMed] [Google Scholar]

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