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. 1997 Jul;17(7):3687–3693. doi: 10.1128/mcb.17.7.3687

A circadian enhancer mediates PER-dependent mRNA cycling in Drosophila melanogaster.

H Hao 1, D L Allen 1, P E Hardin 1
PMCID: PMC232220  PMID: 9199302

Abstract

Genes expressed under circadian-clock control are found in organisms ranging from prokaryotes to humans. In Drosophila melanogaster, the period (per) gene, which is required for clock function, is transcribed in a circadian manner. We have identified a circadian transcriptional enhancer within a 69-bp DNA fragment upstream of the per gene. This enhancer drives high-amplitude mRNA cycling under light-dark-cycling or constant-dark conditions, and this activity is per protein (PER) dependent. An E-box sequence within this 69-bp fragment is necessary for high-level expression, but not for rhythmic expression, indicating that PER mediates circadian transcription through other sequences in this fragment.

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Selected References

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