Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1997 Sep;17(9):5236–5243. doi: 10.1128/mcb.17.9.5236

Transient expression of a winged-helix protein, MNF-beta, during myogenesis.

Q Yang 1, R Bassel-Duby 1, R S Williams 1
PMCID: PMC232374  PMID: 9271401

Abstract

A novel winged-helix transcription factor, MNF-beta, is expressed coincidentally with cell cycle withdrawal and differentiation of skeletal myogenic cells. MNF-beta is closely related to the myocyte nuclear factor (MNF) protein previously described (now termed MNF-alpha), but expression of the two isoforms is differentially regulated, and they exhibit distinctive functional properties with respect to DNA binding in vitro and transcriptional regulatory activity in transient-transfection assays. A DNA sequence motif binding MNF-beta with high affinity was selected from a library of random oligonucleotides and was found to be similar to but distinct from the cognate binding site for HNF-3beta, a more distantly related winged-helix protein. The temporal pattern of MNF-beta expression and the presence of MNF binding motifs within conserved promoter elements of several genes that modulate cell cycle progression support a working hypothesis that MNF proteins may modulate proliferation of myogenic precursor cells during development and muscle regeneration.

Full Text

The Full Text of this article is available as a PDF (1.0 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ang S. L., Rossant J. HNF-3 beta is essential for node and notochord formation in mouse development. Cell. 1994 Aug 26;78(4):561–574. doi: 10.1016/0092-8674(94)90522-3. [DOI] [PubMed] [Google Scholar]
  2. Badiani P., Corbella P., Kioussis D., Marvel J., Weston K. Dominant interfering alleles define a role for c-Myb in T-cell development. Genes Dev. 1994 Apr 1;8(7):770–782. doi: 10.1101/gad.8.7.770. [DOI] [PubMed] [Google Scholar]
  3. Bassel-Duby R., Hernandez M. D., Gonzalez M. A., Krueger J. K., Williams R. S. A 40-kilodalton protein binds specifically to an upstream sequence element essential for muscle-specific transcription of the human myoglobin promoter. Mol Cell Biol. 1992 Nov;12(11):5024–5032. doi: 10.1128/mcb.12.11.5024. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bassel-Duby R., Hernandez M. D., Yang Q., Rochelle J. M., Seldin M. F., Williams R. S. Myocyte nuclear factor, a novel winged-helix transcription factor under both developmental and neural regulation in striated myocytes. Mol Cell Biol. 1994 Jul;14(7):4596–4605. doi: 10.1128/mcb.14.7.4596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Born T. L., Frost J. A., Schönthal A., Prendergast G. C., Feramisco J. R. c-Myc cooperates with activated Ras to induce the cdc2 promoter. Mol Cell Biol. 1994 Sep;14(9):5710–5718. doi: 10.1128/mcb.14.9.5710. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chen X., Rubock M. J., Whitman M. A transcriptional partner for MAD proteins in TGF-beta signalling. Nature. 1996 Oct 24;383(6602):691–696. doi: 10.1038/383691a0. [DOI] [PubMed] [Google Scholar]
  7. Corcoran L. M., Cory S., Adams J. M. Transposition of the immunoglobulin heavy chain enhancer to the myc oncogene in a murine plasmacytoma. Cell. 1985 Jan;40(1):71–79. doi: 10.1016/0092-8674(85)90310-1. [DOI] [PubMed] [Google Scholar]
  8. Costa R. H., Grayson D. R., Darnell J. E., Jr Multiple hepatocyte-enriched nuclear factors function in the regulation of transthyretin and alpha 1-antitrypsin genes. Mol Cell Biol. 1989 Apr;9(4):1415–1425. doi: 10.1128/mcb.9.4.1415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Feldman J. L., Stockdale F. E. Temporal appearance of satellite cells during myogenesis. Dev Biol. 1992 Oct;153(2):217–226. doi: 10.1016/0012-1606(92)90107-r. [DOI] [PubMed] [Google Scholar]
  10. Funk W. D., Wright W. E. Cyclic amplification and selection of targets for multicomponent complexes: myogenin interacts with factors recognizing binding sites for basic helix-loop-helix, nuclear factor 1, myocyte-specific enhancer-binding factor 2, and COMP1 factor. Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9484–9488. doi: 10.1073/pnas.89.20.9484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fürstenau U., Schwaninger M., Blume R., Kennerknecht I., Knepel W. Characterization of a novel protein kinase C response element in the glucagon gene. Mol Cell Biol. 1997 Apr;17(4):1805–1816. doi: 10.1128/mcb.17.4.1805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Grayson J., Williams R. S., Yu Y. T., Bassel-Duby R. Synergistic interactions between heterologous upstream activation elements and specific TATA sequences in a muscle-specific promoter. Mol Cell Biol. 1995 Apr;15(4):1870–1878. doi: 10.1128/mcb.15.4.1870. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Han K., Manley J. L. Functional domains of the Drosophila Engrailed protein. EMBO J. 1993 Jul;12(7):2723–2733. doi: 10.1002/j.1460-2075.1993.tb05934.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hofmann K., Bucher P. The FHA domain: a putative nuclear signalling domain found in protein kinases and transcription factors. Trends Biochem Sci. 1995 Sep;20(9):347–349. doi: 10.1016/s0968-0004(00)89072-6. [DOI] [PubMed] [Google Scholar]
  15. Kaufmann E., Knöchel W. Five years on the wings of fork head. Mech Dev. 1996 Jun;57(1):3–20. doi: 10.1016/0925-4773(96)00539-4. [DOI] [PubMed] [Google Scholar]
  16. Lai E., Clark K. L., Burley S. K., Darnell J. E., Jr Hepatocyte nuclear factor 3/fork head or "winged helix" proteins: a family of transcription factors of diverse biologic function. Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10421–10423. doi: 10.1073/pnas.90.22.10421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lai E., Prezioso V. R., Tao W. F., Chen W. S., Darnell J. E., Jr Hepatocyte nuclear factor 3 alpha belongs to a gene family in mammals that is homologous to the Drosophila homeotic gene fork head. Genes Dev. 1991 Mar;5(3):416–427. doi: 10.1101/gad.5.3.416. [DOI] [PubMed] [Google Scholar]
  18. Megeney L. A., Kablar B., Garrett K., Anderson J. E., Rudnicki M. A. MyoD is required for myogenic stem cell function in adult skeletal muscle. Genes Dev. 1996 May 15;10(10):1173–1183. doi: 10.1101/gad.10.10.1173. [DOI] [PubMed] [Google Scholar]
  19. Overdier D. G., Porcella A., Costa R. H. The DNA-binding specificity of the hepatocyte nuclear factor 3/forkhead domain is influenced by amino-acid residues adjacent to the recognition helix. Mol Cell Biol. 1994 Apr;14(4):2755–2766. doi: 10.1128/mcb.14.4.2755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Parks T. D., Leuther K. K., Howard E. D., Johnston S. A., Dougherty W. G. Release of proteins and peptides from fusion proteins using a recombinant plant virus proteinase. Anal Biochem. 1994 Feb 1;216(2):413–417. doi: 10.1006/abio.1994.1060. [DOI] [PubMed] [Google Scholar]
  21. Philippe J., Morel C., Prezioso V. R. Glucagon gene expression is negatively regulated by hepatocyte nuclear factor 3 beta. Mol Cell Biol. 1994 May;14(5):3514–3523. doi: 10.1128/mcb.14.5.3514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Regier J. L., Shen F., Triezenberg S. J. Pattern of aromatic and hydrophobic amino acids critical for one of two subdomains of the VP16 transcriptional activator. Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):883–887. doi: 10.1073/pnas.90.3.883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sasaki H., Hogan B. L. Differential expression of multiple fork head related genes during gastrulation and axial pattern formation in the mouse embryo. Development. 1993 May;118(1):47–59. doi: 10.1242/dev.118.1.47. [DOI] [PubMed] [Google Scholar]
  24. Sutton J., Costa R., Klug M., Field L., Xu D., Largaespada D. A., Fletcher C. F., Jenkins N. A., Copeland N. G., Klemsz M. Genesis, a winged helix transcriptional repressor with expression restricted to embryonic stem cells. J Biol Chem. 1996 Sep 20;271(38):23126–23133. doi: 10.1074/jbc.271.38.23126. [DOI] [PubMed] [Google Scholar]
  25. Weinstein D. C., Ruiz i Altaba A., Chen W. S., Hoodless P., Prezioso V. R., Jessell T. M., Darnell J. E., Jr The winged-helix transcription factor HNF-3 beta is required for notochord development in the mouse embryo. Cell. 1994 Aug 26;78(4):575–588. doi: 10.1016/0092-8674(94)90523-1. [DOI] [PubMed] [Google Scholar]
  26. Ye H., Kelly T. F., Samadani U., Lim L., Rubio S., Overdier D. G., Roebuck K. A., Costa R. H. Hepatocyte nuclear factor 3/fork head homolog 11 is expressed in proliferating epithelial and mesenchymal cells of embryonic and adult tissues. Mol Cell Biol. 1997 Mar;17(3):1626–1641. doi: 10.1128/mcb.17.3.1626. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. el-Deiry W. S., Tokino T., Waldman T., Oliner J. D., Velculescu V. E., Burrell M., Hill D. E., Healy E., Rees J. L., Hamilton S. R. Topological control of p21WAF1/CIP1 expression in normal and neoplastic tissues. Cancer Res. 1995 Jul 1;55(13):2910–2919. [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES