Skip to main content
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1997 Dec;17(12):6948–6952. doi: 10.1128/mcb.17.12.6948

A heterologous maize rpoB editing site is recognized by transgenic tobacco chloroplasts.

M L Reed 1, M R Hanson 1
PMCID: PMC232552  PMID: 9372927

Abstract

Single nucleotides in plant chloroplast transcripts are edited from the genomically encoded C to U, often resulting in changes of the encoded protein sequence. Site-specific trans-acting factors are postulated to direct the selection of edited residues. In order to further define cis sequences required for RNA editing, we investigated whether two editing sites present in maize rpoB mRNA would be recognized by the editing machinery of transformed tobacco chloroplasts. A 93-nucleotide (nt) segment surrounding site I is sufficient to direct editing of the maize sequence in tobacco chloroplasts. However, an 86-nt segment surrounding maize site IV (which is genomically encoded as a T in tobacco) does not confer editing of this site, suggesting that trans-acting factors necessary for recognition of site IV are not present in tobacco. The maize sequences surrounding site I were found to compete with the endogenous rpoB for a depletable trans factor and to reduce editing of endogenous site I. The presence of exogenous maize site I was also found to decrease editing of endogenous tobacco site II, indicating that there is a shared aspect of editing for some closely spaced editing sites.

Full Text

The Full Text of this article is available as a PDF (679.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison L. A., Simon L. D., Maliga P. Deletion of rpoB reveals a second distinct transcription system in plastids of higher plants. EMBO J. 1996 Jun 3;15(11):2802–2809. [PMC free article] [PubMed] [Google Scholar]
  2. Bock R., Hermann M., Kössel H. In vivo dissection of cis-acting determinants for plastid RNA editing. EMBO J. 1996 Sep 16;15(18):5052–5059. [PMC free article] [PubMed] [Google Scholar]
  3. Bock R., Koop H. U. Extraplastidic site-specific factors mediate RNA editing in chloroplasts. EMBO J. 1997 Jun 2;16(11):3282–3288. doi: 10.1093/emboj/16.11.3282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bock R., Kössel H., Maliga P. Introduction of a heterologous editing site into the tobacco plastid genome: the lack of RNA editing leads to a mutant phenotype. EMBO J. 1994 Oct 3;13(19):4623–4628. doi: 10.1002/j.1460-2075.1994.tb06784.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chaudhuri S., Carrer H., Maliga P. Site-specific factor involved in the editing of the psbL mRNA in tobacco plastids. EMBO J. 1995 Jun 15;14(12):2951–2957. doi: 10.1002/j.1460-2075.1995.tb07295.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chaudhuri S., Maliga P. Sequences directing C to U editing of the plastid psbL mRNA are located within a 22 nucleotide segment spanning the editing site. EMBO J. 1996 Nov 1;15(21):5958–5964. [PMC free article] [PubMed] [Google Scholar]
  7. Chinault A. C., Blakesley V. A., Roessler E., Willis D. G., Smith C. A., Cook R. G., Fenwick R. G., Jr Characterization of transferable plasmids from Shigella flexneri 2a that confer resistance to trimethoprim, streptomycin, and sulfonamides. Plasmid. 1986 Mar;15(2):119–131. doi: 10.1016/0147-619x(86)90048-x. [DOI] [PubMed] [Google Scholar]
  8. Gray M. W., Covello P. S. RNA editing in plant mitochondria and chloroplasts. FASEB J. 1993 Jan;7(1):64–71. doi: 10.1096/fasebj.7.1.8422976. [DOI] [PubMed] [Google Scholar]
  9. Hess W. R., Prombona A., Fieder B., Subramanian A. R., Börner T. Chloroplast rps15 and the rpoB/C1/C2 gene cluster are strongly transcribed in ribosome-deficient plastids: evidence for a functioning non-chloroplast-encoded RNA polymerase. EMBO J. 1993 Feb;12(2):563–571. doi: 10.1002/j.1460-2075.1993.tb05688.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Maier R. M., Zeltz P., Kössel H., Bonnard G., Gualberto J. M., Grienenberger J. M. RNA editing in plant mitochondria and chloroplasts. Plant Mol Biol. 1996 Oct;32(1-2):343–365. doi: 10.1007/BF00039390. [DOI] [PubMed] [Google Scholar]
  11. Smith H. C., Gott J. M., Hanson M. R. A guide to RNA editing. RNA. 1997 Oct;3(10):1105–1123. [PMC free article] [PubMed] [Google Scholar]
  12. Svab Z., Maliga P. High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene. Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):913–917. doi: 10.1073/pnas.90.3.913. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Yoshinaga K., Iinuma H., Masuzawa T., Uedal K. Extensive RNA editing of U to C in addition to C to U substitution in the rbcL transcripts of hornwort chloroplasts and the origin of RNA editing in green plants. Nucleic Acids Res. 1996 Mar 15;24(6):1008–1014. doi: 10.1093/nar/24.6.1008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Zeltz P., Hess W. R., Neckermann K., Börner T., Kössel H. Editing of the chloroplast rpoB transcript is independent of chloroplast translation and shows different patterns in barley and maize. EMBO J. 1993 Nov;12(11):4291–4296. doi: 10.1002/j.1460-2075.1993.tb06113.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Zoubenko O. V., Allison L. A., Svab Z., Maliga P. Efficient targeting of foreign genes into the tobacco plastid genome. Nucleic Acids Res. 1994 Sep 25;22(19):3819–3824. doi: 10.1093/nar/22.19.3819. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES