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. 1996 Jul;178(13):3967–3970. doi: 10.1128/jb.178.13.3967-3970.1996

Use of PCR to isolate genes encoding sigma54-dependent activators from diverse bacteria.

R I Kaufman 1, B T Nixon 1
PMCID: PMC232662  PMID: 8682806

Abstract

Degenerate PCR probes were used to amplify gene fragments encoding the catalytic domain of sigma54-dependent transcription activators. The procedure should be widely applicable, as it recovered both known and novel gene fragments: 5 from Rhizobium meliloti, 13 from Myxococcus xanthus, and 3 from Bacillus subtilis. No fragments were obtained from Synechococcus sp. strain PCC 7002 or Saccharomyces cerevisiae.

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Selected References

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