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. 1996 Jul;178(13):3971–3973. doi: 10.1128/jb.178.13.3971-3973.1996

Structure modification induced in the narG promoter by binding of integration host factor and NARL-P.

X Zhang 1, J A DeMoss 1
PMCID: PMC232663  PMID: 8682807

Abstract

Interaction of integration host factor (IHF) with linear DNA fragments containing the narG promoter region induced an apparent sharp bend in the DNA centered at the IHF-binding site. Binding of NARL-P to two sites adjacent to the IHF site did not induce bending or modify the apparent bending induced by IHF.

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Selected References

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  1. Dong X. R., Li S. F., DeMoss J. A. Upstream sequence elements required for NarL-mediated activation of transcription from the narGHJI promoter of Escherichia coli. J Biol Chem. 1992 Jul 15;267(20):14122–14128. [PubMed] [Google Scholar]
  2. Filutowicz M., Appelt K. The integration host factor of Escherichia coli binds to multiple sites at plasmid R6K gamma origin and is essential for replication. Nucleic Acids Res. 1988 May 11;16(9):3829–3843. doi: 10.1093/nar/16.9.3829. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Goodman S. D., Nash H. A. Functional replacement of a protein-induced bend in a DNA recombination site. Nature. 1989 Sep 21;341(6239):251–254. doi: 10.1038/341251a0. [DOI] [PubMed] [Google Scholar]
  4. Kim S., Landy A. Lambda Int protein bridges between higher order complexes at two distant chromosomal loci attL and attR. Science. 1992 Apr 10;256(5054):198–203. doi: 10.1126/science.1533056. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Li J., Kustu S., Stewart V. In vitro interaction of nitrate-responsive regulatory protein NarL with DNA target sequences in the fdnG, narG, narK and frdA operon control regions of Escherichia coli K-12. J Mol Biol. 1994 Aug 12;241(2):150–165. doi: 10.1006/jmbi.1994.1485. [DOI] [PubMed] [Google Scholar]
  6. Li S. F., DeMoss J. A. Location of sequences in the nar promoter of Escherichia coli required for regulation by Fnr and NarL. J Biol Chem. 1988 Sep 25;263(27):13700–13705. [PubMed] [Google Scholar]
  7. Moitoso de Vargas L., Kim S., Landy A. DNA looping generated by DNA bending protein IHF and the two domains of lambda integrase. Science. 1989 Jun 23;244(4911):1457–1461. doi: 10.1126/science.2544029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nash H. A., Robertson C. A. Purification and properties of the Escherichia coli protein factor required for lambda integrative recombination. J Biol Chem. 1981 Sep 10;256(17):9246–9253. [PubMed] [Google Scholar]
  9. Pérez-Martín J., Rojo F., de Lorenzo V. Promoters responsive to DNA bending: a common theme in prokaryotic gene expression. Microbiol Rev. 1994 Jun;58(2):268–290. doi: 10.1128/mr.58.2.268-290.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rabin R. S., Collins L. A., Stewart V. In vivo requirement of integration host factor for nar (nitrate reductase) operon expression in Escherichia coli K-12. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8701–8705. doi: 10.1073/pnas.89.18.8701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Richet E., Vidal-Ingigliardi D., Raibaud O. A new mechanism for coactivation of transcription initiation: repositioning of an activator triggered by the binding of a second activator. Cell. 1991 Sep 20;66(6):1185–1195. doi: 10.1016/0092-8674(91)90041-v. [DOI] [PubMed] [Google Scholar]
  12. Schleif R. DNA looping. Annu Rev Biochem. 1992;61:199–223. doi: 10.1146/annurev.bi.61.070192.001215. [DOI] [PubMed] [Google Scholar]
  13. Spiro S., Guest J. R. Regulation and over-expression of the fnr gene of Escherichia coli. J Gen Microbiol. 1987 Dec;133(12):3279–3288. doi: 10.1099/00221287-133-12-3279. [DOI] [PubMed] [Google Scholar]
  14. Stewart V. Nitrate regulation of anaerobic respiratory gene expression in Escherichia coli. Mol Microbiol. 1993 Aug;9(3):425–434. doi: 10.1111/j.1365-2958.1993.tb01704.x. [DOI] [PubMed] [Google Scholar]
  15. Stewart V. Nitrate respiration in relation to facultative metabolism in enterobacteria. Microbiol Rev. 1988 Jun;52(2):190–232. doi: 10.1128/mr.52.2.190-232.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Thompson J. F., Landy A. Empirical estimation of protein-induced DNA bending angles: applications to lambda site-specific recombination complexes. Nucleic Acids Res. 1988 Oct 25;16(20):9687–9705. doi: 10.1093/nar/16.20.9687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Walker M. S., DeMoss J. A. NarL-phosphate must bind to multiple upstream sites to activate transcription from the narG promoter of Escherichia coli. Mol Microbiol. 1994 Nov;14(4):633–641. doi: 10.1111/j.1365-2958.1994.tb01302.x. [DOI] [PubMed] [Google Scholar]
  18. Walker M. S., DeMoss J. A. Phosphorylation and dephosphorylation catalyzed in vitro by purified components of the nitrate sensing system, NarX and NarL. J Biol Chem. 1993 Apr 25;268(12):8391–8393. [PubMed] [Google Scholar]
  19. Walker M. S., DeMoss J. A. Promoter sequence requirements for Fnr-dependent activation of transcription of the narGHJI operon. Mol Microbiol. 1991 Feb;5(2):353–360. doi: 10.1111/j.1365-2958.1991.tb02116.x. [DOI] [PubMed] [Google Scholar]
  20. Wu H. M., Crothers D. M. The locus of sequence-directed and protein-induced DNA bending. Nature. 1984 Apr 5;308(5959):509–513. doi: 10.1038/308509a0. [DOI] [PubMed] [Google Scholar]

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