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The effects of GT1b on B cells
B cells (2×105 cells/200 μl/well) from five different donors were cultured in triplicate in the presence or absence of GT1b (10 μM).Immunoglobulin production was determined by enzyme-linked immunosorbent assay (ELISA) on day 5, and [3H]TdR uptake was evaluated on day 4. Values are expressed as mean±SD (n = 5). SDs of triplicate cultures from individual donors were <10% of the means.
