Figure 1.
Introduction of mutations into the 3′ coding region of the mouse μ gene. The Bam HI/KpnI fragment of the μ gene was the target for the introduction of mutations (shaded) into the Cμ4 exon. Following the confirmation of sequence the Bst EII/Kpn I fragment was cloned back into the parental Sp6(C414) and Sp6(S414) genes as shown. The asterisk (*) indicates Ser 414.