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. 1999 Oct;98(2):303–311. doi: 10.1046/j.1365-2567.1999.00859.x

Figure 6.

Figure 6

Specific interaction of mouse CD97 with mouse DAF. HEK 293 cells were transfected with a pCDNA3 plasmid containing mouse CD97 cDNA either in the sense or antisence orientation. Erythrocyte and splenocyte-binding assays were performed 48 hr later as described in the Materials and Methods. (a) Normal mouse erythrocytes bound to HEK 293 cells transfected with mouse CD97 cDNA in the sense orientation. (b) Normal mouse erythrocytes failed to bind to HEK 293 cells transfected with mouse CD97 cDNA in the antisense orientation. (c) In contrast to wild-type mouse erythrocytes (WT), DAF knockout mouse erythroctes (KO) and human erythrocytes (hE) did not bind to HEK 293 cells transfected with mouse CD97 cDNA either in the sense (filled bar) or antisense (open bar) orientation). (d) Normal but not DAF knock-out mouse splenocytes had higher binding to HEK 293 cells transfected with mouse CD97 cDNA in the sense orientation (filled bar) than to cells transfected with the cDNA in the antisense orientation (open bar).