Table 1.
Antibody responses to immunization with attenuated herpes simplex virus type‐2 (HSV‐2)
| Log2.5 GMT and GMT of IgG anti‐HSV‐2 in: | |||||
|---|---|---|---|---|---|
| Site of immunization | Serum* | Vagina | Log2.5 GMT ratio and GMT ratio of IgG2a/IgG1 anti‐HSV‐2 titres in serum† | Vaginal secretion/serum ratio of IgG anti‐HSV‐2 titres (10−3)‡ | Log3 GMT and GMT of S‐IgA anti‐HSV‐2 in the vagina§ |
| Vaginal‐DP | 11.5±0.16 | 4.8±0.23 | 0.50±0.30 | 2.5±0.49 | 4.0±0.24 |
| (38000) | (81) | (1.6) | (80) | ||
| Vaginal‐E‐scar | 11.5±0.17 | 4.1±0.25 | 1.3±0.23 | 1.8±0.73 | 3.1±0.30 |
| (38000) | (43) | (3.2) | (29) | ||
| Nasal | 10.8±0.13 | 2.9±0.21 | 0.59±0.33 | 0.80±0.12 | 3.0±0.30 |
| (21000) | (14) | (1.7) | (26) | ||
The mean serum immunoglobulin G (IgG) anti‐HSV‐2 titre in the nasal immunization group was significantly lower than in either vaginal immunization group (P = 0.0076; three‐group ANOVA).
The IgG2a and IgG1 anti‐HSV‐2 titres were measured, and their ratios were calculated for each mouse. For each group, the log2.5 geometric mean titer (GMT) ratio and its standard error was calculated and used for statistical comparison of groups. The GMT ratios are presented in parentheses. The IgG2a/IgG1 anti‐HSV‐2 titre ratios were not significantly different among the three immnized groups (P = 0.15; three‐group analysis of variance ANOVA).
The mean vaginal secretion/serum IgG anti‐HSV‐2 titre ratios were significantly different (P = 0.0079; Kruskal–Wallis non‐parametric ANOVA).
These titres cannot be directly compared to IgG titres because different tirre end‐points were used. S‐IgA, secretory immunoglobulin A.