Figure 5.

Differences in peptide specificity and between A^k/b- and A^k/k-derived T cells. T-cell lines were generated using lymph node (LN) cells from 30 µg of the recombinant 16 000-MW protein of Mycobacterium tuberculosis (rPT16)-primed B10.BR (a) and (B10.BR × B10)F₁ (b) mice. Cultured T cells were stimulated with rPT16 and with six different synthetic 20-mer peptides (30 µg/ml) derived from the PT16 sequence. Cultures were supplemented with irradiated spleen cells from non-immune B10.BR, (B10.BR × B10)F₁ hybrid, or B10 mice.