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. 1997 May;35(5):1231–1235. doi: 10.1128/jcm.35.5.1231-1235.1997

Survey of emm gene sequences and T-antigen types from systemic Streptococcus pyogenes infection isolates collected in San Francisco, California; Atlanta, Georgia; and Connecticut in 1994 and 1995.

B Beall 1, R Facklam 1, T Hoenes 1, B Schwartz 1
PMCID: PMC232734  PMID: 9114412

Abstract

The variable 5' emm (M-protein gene) sequences and T-antigen types were determined from 340 systemic group A streptococcal (GAS) isolates taken from hospitalized patients in San Francisco, Calif.; Atlanta, Ga.; and Connecticut in 1994 and 1995. Eighty percent of these isolates had emm sequences and T-antigen types in agreement with previously recorded M- and T-antigen associations. Most of the remaining strains either were T nontypeable (11%) or contained emm genes encoding M proteins for which T-antigen associations have not been made (6%). One newly encountered emm gene, designated ST2974, from each of 13 isolates had the T type 8/25/Imp19. Another new emm gene, ST2967, from 8 of 11 isolates was T nontypeable. Six other unique emm gene sequences from seven isolates were encountered. Sequencing of the variable region of the emm gene of GAS isolates (emm typing) is effective for surveying the sequence variability of the M virulence protein, and combined with T typing, emm typing is useful for monitoring GAS strain diversity.

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Selected References

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  1. Beachey E. H., Seyer J. M., Dale J. B., Simpson W. A., Kang A. H. Type-specific protective immunity evoked by synthetic peptide of Streptococcus pyogenes M protein. Nature. 1981 Jul 30;292(5822):457–459. doi: 10.1038/292457a0. [DOI] [PubMed] [Google Scholar]
  2. Beall B., Facklam R., Thompson T. Sequencing emm-specific PCR products for routine and accurate typing of group A streptococci. J Clin Microbiol. 1996 Apr;34(4):953–958. doi: 10.1128/jcm.34.4.953-958.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bessen D. E., Sotir C. M., Readdy T. L., Hollingshead S. K. Genetic correlates of throat and skin isolates of group A streptococci. J Infect Dis. 1996 Apr;173(4):896–900. doi: 10.1093/infdis/173.4.896. [DOI] [PubMed] [Google Scholar]
  4. Boyle M. D., Hawlitzky J., Raeder R., Podbielski A. Analysis of genes encoding two unique type IIa immunoglobulin G-binding proteins expressed by a single group A streptococcal isolate. Infect Immun. 1994 Apr;62(4):1336–1347. doi: 10.1128/iai.62.4.1336-1347.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Colman G., Tanna A., Efstratiou A., Gaworzewska E. T. The serotypes of Streptococcus pyogenes present in Britain during 1980-1990 and their association with disease. J Med Microbiol. 1993 Sep;39(3):165–178. doi: 10.1099/00222615-39-3-165. [DOI] [PubMed] [Google Scholar]
  6. Fischetti V. A. Streptococcal M protein: molecular design and biological behavior. Clin Microbiol Rev. 1989 Jul;2(3):285–314. doi: 10.1128/cmr.2.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GOODER H. Association of a serum opacity reaction with serological type in Streptococcus pyogenes. J Gen Microbiol. 1961 Jul;25:347–352. doi: 10.1099/00221287-25-3-347. [DOI] [PubMed] [Google Scholar]
  8. Gardiner D., Hartas J., Currie B., Mathews J. D., Kemp D. J., Sriprakash K. S. Vir typing: a long-PCR typing method for group A streptococci. PCR Methods Appl. 1995 Apr;4(5):288–293. doi: 10.1101/gr.4.5.288. [DOI] [PubMed] [Google Scholar]
  9. Johnson D. R., Kaplan E. L. A review of the correlation of T-agglutination patterns and M-protein typing and opacity factor production in the identification of group A streptococci. J Med Microbiol. 1993 May;38(5):311–315. doi: 10.1099/00222615-38-5-311. [DOI] [PubMed] [Google Scholar]
  10. Jones K. F., Fischetti V. A. The importance of the location of antibody binding on the M6 protein for opsonization and phagocytosis of group A M6 streptococci. J Exp Med. 1988 Mar 1;167(3):1114–1123. doi: 10.1084/jem.167.3.1114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kaufhold A., Podbielski A., Baumgarten G., Blokpoel M., Top J., Schouls L. Rapid typing of group A streptococci by the use of DNA amplification and non-radioactive allele-specific oligonucleotide probes. FEMS Microbiol Lett. 1994 Jun 1;119(1-2):19–25. doi: 10.1111/j.1574-6968.1994.tb06861.x. [DOI] [PubMed] [Google Scholar]
  12. LANCEFIELD R. C. Current knowledge of type-specific M antigens of group A streptococci. J Immunol. 1962 Sep;89:307–313. [PubMed] [Google Scholar]
  13. MOODY M. D., PADULA J., LIZANA D., HALL C. T. EPIDEMIOLOGIC CHARACTERIZATION OF GROUP A STREPTOCOCCI BY T-AGGLUTINATION AND M-PRECIPITATION TESTS IN THE PUBLIC HEALTH LABORATORY. Health Lab Sci. 1965 Jul;2:149–162. [PubMed] [Google Scholar]
  14. Maxted W. R., Widdowson J. P., Fraser C. A., Ball L. C., Bassett D. C. The use of the serum opacity reaction in the typing of group-A streptococci. J Med Microbiol. 1973 Feb;6(1):83–90. doi: 10.1099/00222615-6-1-83. [DOI] [PubMed] [Google Scholar]
  15. Musser J. M., Kapur V., Szeto J., Pan X., Swanson D. S., Martin D. R. Genetic diversity and relationships among Streptococcus pyogenes strains expressing serotype M1 protein: recent intercontinental spread of a subclone causing episodes of invasive disease. Infect Immun. 1995 Mar;63(3):994–1003. doi: 10.1128/iai.63.3.994-1003.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Podbielski A., Melzer B., Lütticken R. Application of the polymerase chain reaction to study the M protein(-like) gene family in beta-hemolytic streptococci. Med Microbiol Immunol. 1991;180(4):213–227. doi: 10.1007/BF00215250. [DOI] [PubMed] [Google Scholar]
  17. Relf W. A., Martin D. R., Sriprakash K. S. Identification of sequence types among the M-nontypeable group A streptococci. J Clin Microbiol. 1992 Dec;30(12):3190–3194. doi: 10.1128/jcm.30.12.3190-3194.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schwartz B., Facklam R. R., Breiman R. F. Changing epidemiology of group A streptococcal infection in the USA. Lancet. 1990 Nov 10;336(8724):1167–1171. doi: 10.1016/0140-6736(90)92777-f. [DOI] [PubMed] [Google Scholar]
  19. Single L. A., Martin D. R. Clonal differences within M-types of the group A Streptococcus revealed by pulsed field gel electrophoresis. FEMS Microbiol Lett. 1992 Feb 1;70(1):85–89. doi: 10.1016/0378-1097(92)90567-8. [DOI] [PubMed] [Google Scholar]
  20. Upton M., Carter P. E., Morgan M., Edwards G. F., Pennington T. H. Clonal structure of invasive Streptococcus pyogenes in Northern Scotland. Epidemiol Infect. 1995 Oct;115(2):231–241. doi: 10.1017/s0950268800058362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Whatmore A. M., Kapur V., Musser J. M., Kehoe M. A. Molecular population genetic analysis of the enn subdivision of group A streptococcal emm-like genes: horizontal gene transfer and restricted variation among enn genes. Mol Microbiol. 1995 Mar;15(6):1039–1048. doi: 10.1111/j.1365-2958.1995.tb02279.x. [DOI] [PubMed] [Google Scholar]
  22. Whatmore A. M., Kapur V., Sullivan D. J., Musser J. M., Kehoe M. A. Non-congruent relationships between variation in emm gene sequences and the population genetic structure of group A streptococci. Mol Microbiol. 1994 Nov;14(4):619–631. doi: 10.1111/j.1365-2958.1994.tb01301.x. [DOI] [PubMed] [Google Scholar]
  23. Whatmore A. M., Kehoe M. A. Horizontal gene transfer in the evolution of group A streptococcal emm-like genes: gene mosaics and variation in Vir regulons. Mol Microbiol. 1994 Jan;11(2):363–374. doi: 10.1111/j.1365-2958.1994.tb00316.x. [DOI] [PubMed] [Google Scholar]

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