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. 1997 May;35(5):1281–1283. doi: 10.1128/jcm.35.5.1281-1283.1997

Evaluation of a line probe assay kit for characterization of rpoB mutations in rifampin-resistant Mycobacterium tuberculosis isolates from New York City.

R C Cooksey 1, G P Morlock 1, S Glickman 1, J T Crawford 1
PMCID: PMC232749  PMID: 9114427

Abstract

A commercial line probe assay kit (Inno-LiPA Rif.TB) for rapid identification of mutations in the rpoB gene associated with rifampin resistance in Mycobacterium tuberculosis was evaluated with a collection of 51 rifampin-resistant strains. Nine distinct rpoB mutations were identified. Concordances with automated sequence results for five wild-type kit probes and four probes for specific mutations were 94.1 and 100%, respectively. Overall concordance of the line probe assay kit with phenotypic rifampin susceptibility testing results was 90.2%.

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Selected References

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