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. 1997 May;35(5):1284–1286. doi: 10.1128/jcm.35.5.1284-1286.1997

Detection of genetically diverse human immunodeficiency virus type 1 group M and O isolates by PCR.

R A Respess 1, A Butcher 1, H Wang 1, T Chaowanachan 1, N Young 1, N Shaffer 1, T D Mastro 1, B Biryahwaho 1, R Downing 1, A Tanuri 1, M Schechter 1, R Pascu 1, L Zekeng 1, L Kaptué 1, L Gürtler 1, J Eberle 1, D Ellenberger 1, C Fridlund 1, M Rayfield 1, S Kwok 1
PMCID: PMC232750  PMID: 9114428

Abstract

A panel of 136 genetically diverse group M and 5 group O adult isolates from outside the United States and Europe were evaluated by PCR with the Roche AMPLICOR HIV-1 test, a modified version of the AMPLICOR HIV-1 test, and a new primer pair/probe system. Detection of some of these isolates was less efficient with the AMPLICOR HIV-1 test; however, the assay was significantly improved by reducing the sample input and lowering the annealing temperature. The new primer pair/probe set detected 140 of 141 isolates, including the 5 group O isolates that were not detected with either of the AMPLICOR HIV-1 test formats.

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Selected References

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