Abstract
A procedure is presented for the rapid screening of bacterial colonies to detect mutants unable to produce 14CO2 from a labeled precursor. The method is especially useful for mass screening for mutants that cannot be easily detected by their phenotypic characteristics.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cunningham-Rundles S., Maas W. K. Isolation, characterization, and mapping of Escherichia coli mutants blocked in the synthesis of ornithine decarboxylase. J Bacteriol. 1975 Nov;124(2):791–799. doi: 10.1128/jb.124.2.791-799.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morris D. R., Koffron K. L. Urea production and putrescine biosynthesis by Escherichia coli. J Bacteriol. 1967 Nov;94(5):1516–1519. doi: 10.1128/jb.94.5.1516-1519.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morris D. R., Pardee A. B. Multiple pathways of putrescine biosynthesis in Escherichia coli. J Biol Chem. 1966 Jul 10;241(13):3129–3135. [PubMed] [Google Scholar]
- Weiss B., Milcarek C. Mass screening for mutants with altered DNases by microassay techniques. Methods Enzymol. 1974;29:180–193. doi: 10.1016/0076-6879(74)29020-7. [DOI] [PubMed] [Google Scholar]
- Wickner R. B., Tabor C. W., Tabor H. Purification of adenosylmethionine decarboxylase from Escherichia coli W: evidence for covalently bound pyruvate. J Biol Chem. 1970 Apr 25;245(8):2132–2139. [PubMed] [Google Scholar]