Abstract
We have observed that transcription, involved in production of allophanate hydrolase, is completed 2.5 min after the addition of inducer at 15 degrees C. The rna1 gene product must be functional up unti 10 min; protein synthesis is initiated at 20 min and is terminated by 24 min. Two minutes later, active enzyme appears. The results confirm our earlier observations and eliminate any uncertainty that might have clouded identification of the time within the lag period that is occupied by ribonucleic acid synthesis.
Full text
PDF![498](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/232883/e0ad6308cc27/jbacter00311-0508.png)
![499](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/232883/b2e0b80635a9/jbacter00311-0509.png)
![500](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/232883/a9e68ac03df4/jbacter00311-0510.png)
![501](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/232883/a02489c03d5b/jbacter00311-0511.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bossinger J., Cooper T. G. Sequence of molecular events involved in induction of allophanate hydrolase. J Bacteriol. 1976 Apr;126(1):198–204. doi: 10.1128/jb.126.1.198-204.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartwell L. H., Hutchison H. T., Holland T. M., McLaughlin C. S. The effect of cycloheximide upon polyribosome stability in two yeast mutants defective respectively in the initiation of polypeptide chains and in messenger RNA synthesis. Mol Gen Genet. 1970;106(4):347–361. doi: 10.1007/BF00324052. [DOI] [PubMed] [Google Scholar]
- Hartwell L. H., McLaughlin C. S. A mutant of yeast apparently defective in the initiation of protein synthesis. Proc Natl Acad Sci U S A. 1969 Feb;62(2):468–474. doi: 10.1073/pnas.62.2.468. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KEPES A. KINETICS OF INDUCED ENZYME SYNTHESIS. DETERMINATION OF THE MEAN LIFE OF GALACTOSIDASE-SPECIFIC MESSENGER RNA. Biochim Biophys Acta. 1963 Oct 15;76:293–309. [PubMed] [Google Scholar]
- Lawther R. P., Cooper T. G. Kinetics of induced and repressed enzyme synthesis in Saccharomyces cerevisiae. J Bacteriol. 1975 Mar;121(3):1064–1073. doi: 10.1128/jb.121.3.1064-1073.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lawther R. P., Phillips S. L., Cooper T. G. Lomofungin inhibition of allophanate hydrolase synthesis in Saccharomyces cerevisiae. Mol Gen Genet. 1975;137(2):89–99. doi: 10.1007/BF00341675. [DOI] [PubMed] [Google Scholar]
- Whitney P. A., Cooper T. G. Urea carboxylase and allophanate hydrolase. Two components of adenosine triphosphate:urea amido-lyase in Saccharomyces cerevisiae. J Biol Chem. 1972 Mar 10;247(5):1349–1353. [PubMed] [Google Scholar]
- Wickerham L. J. A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts. J Bacteriol. 1946 Sep;52(3):293–301. [PMC free article] [PubMed] [Google Scholar]