Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1976 Sep;127(3):1047–1057. doi: 10.1128/jb.127.3.1047-1057.1976

Genetic control of the glp system in Bacillus subtilis.

V Lindgren, L Rutberg
PMCID: PMC232894  PMID: 182672

Abstract

In pleiotropic negative glycerol utilization mutants (GlpPI mutants) of Bacillus subitilis, glycerol kinase and sn-glycerol 3-phosphate (G3P) dehydrogenase are noninducible. GlpPI mutants also fail to take up exogenous [14C]G3P. To study the regulation of the glp system in B. subtilis phenotypically, Glp+ revertants were isolated from GlpPI mutants. Four classes of revertants were identified: phenotypically, wild type; R1 type, which contains an informational suppressor, R2 type, which produced G3P dehydrogenase constitutively; and R3 type, with a temperature-sensitive Glp phenotype producing G3P dehydrogenase constitutively at permissive temperature (32 degrees C). The properties of the revertants indicate that the glpPI locus codes for a protein with a positive regulatory function.

Full text

PDF
1047

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anagnostopoulos C., Spizizen J. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1961 May;81(5):741–746. doi: 10.1128/jb.81.5.741-746.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berman-Kurtz M., Lin E. C., Richey D. P. Promoter-like mutant with increased expression of the glycerol kinase operon of Escherichia coli. J Bacteriol. 1971 Jun;106(3):724–731. doi: 10.1128/jb.106.3.724-731.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cozzarelli N. R., Freedberg W. B., Lin E. C. Genetic control of L-alpha-glycerophosphate system in Escherichia coli. J Mol Biol. 1968 Feb 14;31(3):371–387. doi: 10.1016/0022-2836(68)90415-4. [DOI] [PubMed] [Google Scholar]
  4. Cozzarelli N. R., Lin E. C. Chromosomal location of the structural gene for glycerol kinase in Escherichia coli. J Bacteriol. 1966 May;91(5):1763–1766. doi: 10.1128/jb.91.5.1763-1766.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Englesberg E., Squires C., Meronk F., Jr The L-arabinose operon in Escherichia coli B-r: a genetic demonstration of two functional states of the product of a regulator gene. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1100–1107. doi: 10.1073/pnas.62.4.1100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Freedberg W. B., Lin E. C. Three kinds of controls affecting the expression of the glp regulon in Escherichia coli. J Bacteriol. 1973 Sep;115(3):816–823. doi: 10.1128/jb.115.3.816-823.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GAREN A., LEVINTHAL C. A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase. Biochim Biophys Acta. 1960 Mar 11;38:470–483. doi: 10.1016/0006-3002(60)91282-8. [DOI] [PubMed] [Google Scholar]
  8. Gay P., Cordier P., Marquet M., Delobbe A. Carbohydrate metabolism and transport in Bacillus subtilis. A study of ctr mutations. Mol Gen Genet. 1973 Mar 19;121(4):355–368. doi: 10.1007/BF00433234. [DOI] [PubMed] [Google Scholar]
  9. Gorini L., Beckwith J. R. Suppression. Annu Rev Microbiol. 1966;20:401–422. doi: 10.1146/annurev.mi.20.100166.002153. [DOI] [PubMed] [Google Scholar]
  10. Guerola N., Ingraham J. L., Cerdá-Olmedo E. Induction of closely linked multiple mutations by nitrosoguanidine. Nat New Biol. 1971 Mar 24;230(12):122–125. doi: 10.1038/newbio230122a0. [DOI] [PubMed] [Google Scholar]
  11. HAYASHI S., KOCH J. P., LIN E. C. ACTIVE TRANSPORT OF L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI. J Biol Chem. 1964 Sep;239:3098–3105. [PubMed] [Google Scholar]
  12. HIRSCHMANN H. The nature of substrate asymmetry in stereoselective reactions. J Biol Chem. 1960 Oct;235:2762–2767. [PubMed] [Google Scholar]
  13. Hendlin D., Stapley E. O., Jackson M., Wallick H., Miller A. K., Wolf F. J., Miller T. W., Chaiet L., Kahan F. M., Foltz E. L. Phosphonomycin, a new antibiotic produced by strains of streptomyces. Science. 1969 Oct 3;166(3901):122–123. doi: 10.1126/science.166.3901.122. [DOI] [PubMed] [Google Scholar]
  14. KOCH J. P., HAYASHI S., LIN E. C. THE CONTROL OF DISSIMILATION OF GLYCEROL AND L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI. J Biol Chem. 1964 Sep;239:3106–3108. [PubMed] [Google Scholar]
  15. Kahan F. M., Kahan J. S., Cassidy P. J., Kropp H. The mechanism of action of fosfomycin (phosphonomycin). Ann N Y Acad Sci. 1974 May 10;235(0):364–386. doi: 10.1111/j.1749-6632.1974.tb43277.x. [DOI] [PubMed] [Google Scholar]
  16. Kistler W. S., Lin E. C. Anaerobic L- -glycerophosphate dehydrogenase of Escherichia coli: its genetic locus and its physiological role. J Bacteriol. 1971 Dec;108(3):1224–1234. doi: 10.1128/jb.108.3.1224-1234.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. LIN E. C., KOCH J. P., CHUSED T. M., JORGENSEN S. E. Utilization of L-alpha-glycerophosphate by Escherichia coli without hydrolysis. Proc Natl Acad Sci U S A. 1962 Dec 15;48:2145–2150. doi: 10.1073/pnas.48.12.2145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  19. Le Hégarat J. C., Anagnostopoulos C. Purification, subunit structure and properties of two repressible phosphohydrolases of Bacillus subtilis. Eur J Biochem. 1973 Nov 15;39(2):525–539. doi: 10.1111/j.1432-1033.1973.tb03151.x. [DOI] [PubMed] [Google Scholar]
  20. Lindgren V., Rutberg L. Glycerol metabolism in Bacillus subtilis: gene-enzyme relationships. J Bacteriol. 1974 Aug;119(2):431–442. doi: 10.1128/jb.119.2.431-442.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Miki T., Minami Z., Ikeda Y. The genetics of alkaline phosphatase formation in Bacillus subtilis. Genetics. 1965 Nov;52(5):1093–1100. doi: 10.1093/genetics/52.5.1093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Mindich L. Membrane synthesis in Bacillus subtilis. I. Isolation and properties of strains bearing mutations in glycerol metabolism. J Mol Biol. 1970 Apr 28;49(2):415–432. doi: 10.1016/0022-2836(70)90254-8. [DOI] [PubMed] [Google Scholar]
  23. Mindich L. Pathway for oxidative dissimilation of glycerol in Bacillur subtilis. J Bacteriol. 1968 Aug;96(2):565–566. doi: 10.1128/jb.96.2.565-566.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. O Y. K., Freese E. B., Freese E. Abnormal septation and inhibition of sporulation by accumulation of L- -glycerophosphate in Bacillus subtilis mutants. J Bacteriol. 1973 Feb;113(2):1034–1045. doi: 10.1128/jb.113.2.1034-1045.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Power J., Irr J. Regulatory gene control of transcription of the L-arabinose operon in Escherichia coli. J Biol Chem. 1973 Nov 25;248(22):7806–7810. [PubMed] [Google Scholar]
  26. Saheb S. A. Etude de deux mutants du métabolisme du glycérol chez Bacillus subtilis. Can J Microbiol. 1972 Aug;18(8):1315–1325. [PubMed] [Google Scholar]
  27. Saheb S. A. Perméation du glycérol et sporulation chez Bacillus subtilis. Can J Microbiol. 1972 Aug;18(8):1307–1313. [PubMed] [Google Scholar]
  28. Young F. E., Smith C., Reilly B. E. Chromosomal location of genes regulating resistance to bacteriophage in Bacillus subtilis. J Bacteriol. 1969 Jun;98(3):1087–1097. doi: 10.1128/jb.98.3.1087-1097.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES