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. 1976 Sep;127(3):1307–1314. doi: 10.1128/jb.127.3.1307-1314.1976

Role of deoxyribonucleic acid polymerase III in the repair of single-strand breaks produced in Escherichia coli deoxyribonucleic acid by gamma radiation.

C Hamelin, D A Youngs, K C Smith
PMCID: PMC232925  PMID: 783140

Abstract

Cell survival, deoxyribonucleic acid (DNA) degradation, and the repair of DNA single-strand breaks were measured for Escherichia coli K-12 pol+, polA1, polC1026(ts), and polA1 polC1026(ts) cells after 137Cs gamma irradiation. The results indicate that DNA polymerase III is required for growth medium-dependent (type III) repair in polA+ or polA cells. In pol+ or polC cells, DNA polymerase I performs type II repair efficiently. The relative deficiencies of each of these strains in DNA repair generally correlate with their relative sensitivities to cell killing and with the extent of DNA degradation observed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonura T., Smith K. C., Kaplan H. S. Enzymatic induction of DNA double-strand breaks in gamma-irradiated Escherichia coli K-12. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4265–4269. doi: 10.1073/pnas.72.11.4265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bonura T., Smith K. C. Quantitative evidence for enzymatically-indeced DNA double-strand breaks as lethal lesions in UV irradiated pol+ and polA1 strains of E. coli K-12. Photochem Photobiol. 1975 Dec;22(6):243–248. doi: 10.1111/j.1751-1097.1975.tb06743.x. [DOI] [PubMed] [Google Scholar]
  3. Bridges B. A., Ashwood-Smith M. J., Munson R. J. Correlation of bacterial sensitivities to ionizing radiation and mild heating. J Gen Microbiol. 1969 Sep;58(1):115–124. doi: 10.1099/00221287-58-1-115. [DOI] [PubMed] [Google Scholar]
  4. De Lucia P., Cairns J. Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969 Dec 20;224(5225):1164–1166. doi: 10.1038/2241164a0. [DOI] [PubMed] [Google Scholar]
  5. Ganesan A. K., Smith K. C. Dark recovery processes in Escherichia coli irradiated with ultraviolet light. I. Effect of rec mutations on liquid holding recovery. J Bacteriol. 1968 Aug;96(2):365–373. doi: 10.1128/jb.96.2.365-373.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gefter M. L., Hirota Y., Kornberg T., Wechsler J. A., Barnoux C. Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3150–3153. doi: 10.1073/pnas.68.12.3150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hurwitz J., Wickner S. Involvement of two protein factors and ATP in in vitro DNA synthesis catalyzed by DNA polymerase 3 of Escherichia coli. Proc Natl Acad Sci U S A. 1974 Jan;71(1):6–10. doi: 10.1073/pnas.71.1.6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Knippers R. DNA polymerase II. Nature. 1970 Dec 12;228(5276):1050–1053. doi: 10.1038/2281050a0. [DOI] [PubMed] [Google Scholar]
  9. Kornberg T., Gefter M. L. DNA synthesis in cell-free extracts of a DNA polymerase-defective mutant. Biochem Biophys Res Commun. 1970 Sep 30;40(6):1348–1355. doi: 10.1016/0006-291x(70)90014-8. [DOI] [PubMed] [Google Scholar]
  10. Kornberg T., Gefter M. L. Deoxyribonucleic acid synthesis in cell-free extracts. IV. Purification and catalytic properties of deoxyribonucleic acid polymerase III. J Biol Chem. 1972 Sep 10;247(17):5369–5375. [PubMed] [Google Scholar]
  11. Kornberg T., Gefter M. L. Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II. Proc Natl Acad Sci U S A. 1971 Apr;68(4):761–764. doi: 10.1073/pnas.68.4.761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Levin D., Hutchinson F. Neutral sucrose sedimentation of very large DNA from Bacillus subtilis. I. Effect of random double-strand breaks and centrifuge speed on sedimentation. J Mol Biol. 1973 Apr 15;75(3):455–478. doi: 10.1016/0022-2836(73)90454-3. [DOI] [PubMed] [Google Scholar]
  13. Livingston D. M., Richardson C. C. Deoxyribonucleic acid polymerase III of Escherichia coli. Characterization of associated exonuclease activities. J Biol Chem. 1975 Jan 25;250(2):470–478. [PubMed] [Google Scholar]
  14. Moses R. E., Moody E. E. DNA repair synthesis dependent on the uvrA,B gene products in toluene-treated cells. J Biol Chem. 1975 Oct 25;250(20):8055–8061. [PubMed] [Google Scholar]
  15. Moses R. E., Richardson C. C. A new DNA polymerase activity of Escherichia coli. I. Purification and properties of the activity present in E. coli polA1. Biochem Biophys Res Commun. 1970 Dec 24;41(6):1557–1564. doi: 10.1016/0006-291x(70)90565-6. [DOI] [PubMed] [Google Scholar]
  16. Nüsslein V., Otto B., Bonhoeffer F., Schaller H. Function of DNA polymerase 3 in DNA replication. Nat New Biol. 1971 Dec 29;234(52):285–286. doi: 10.1038/newbio234285a0. [DOI] [PubMed] [Google Scholar]
  17. Sedgwick S. G., Bridges B. A. Evidence for indirect production of DNA strand scissions during mild heating of Escherichia coli. J Gen Microbiol. 1972 Jun;71(1):191–193. doi: 10.1099/00221287-71-1-191. [DOI] [PubMed] [Google Scholar]
  18. Sedgwick S. G., Bridges B. A. Requirement for either DNA polymerase I or DNA polymerase 3 in post-replication repair in excision-proficient Escherichia coli. Nature. 1974 May 24;249(455):348–349. doi: 10.1038/249348a0. [DOI] [PubMed] [Google Scholar]
  19. Smith K. C., Youngs D. A., Van der Schueren E. Dependence upon growth medium and the polA, polC, recA, recB, recC, and exrA genes of separate branches of the uvr Gene-dependent excision-repair process in Escherichia coli K12 cells. Basic Life Sci. 1975;5B:443–451. doi: 10.1007/978-1-4684-2898-8_4. [DOI] [PubMed] [Google Scholar]
  20. Tait R. C., Harris A. L., Smith D. W. DNA repair in Escherichia coli mutants deficient in DNA polymerases I, II and-or 3. Proc Natl Acad Sci U S A. 1974 Mar;71(3):675–679. doi: 10.1073/pnas.71.3.675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tomilin N. V. Repair of gamma-ray induced lesions in E. coli cells deficient in DNA polymerase I and having thermosensitive DNA polymerase 3. Mol Gen Genet. 1974 Mar 14;129(2):97–103. doi: 10.1007/BF00268624. [DOI] [PubMed] [Google Scholar]
  22. Tomilin N. V., Svetlova M. P. On the mechanism of post-replication repair in Escherichia coli cells: the role of DNA polymerase 3. FEBS Lett. 1974 Jul 15;43(2):185–188. doi: 10.1016/0014-5793(74)80996-8. [DOI] [PubMed] [Google Scholar]
  23. Town C. D., Smith K. C., Kaplan H. S. Production and repair of radiochemical damage in Escherichia coli deoxyribonucleic acid; its modification by culture conditions and relation to survival. J Bacteriol. 1971 Jan;105(1):127–135. doi: 10.1128/jb.105.1.127-135.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Town C. D., Smith K. C., Kaplan H. S. Repair of X-ray damage to bacterial DNA. Curr Top Radiat Res Q. 1973 Jul;8(4):351–399. [PubMed] [Google Scholar]
  25. Town C. D., Smith K. C., Kaplan H. S. The rapair of DNA single-strand breaks in E. coli K-12 x-irradiated in the presence or absence of oxygen; the influence of repair on cell survival. Radiat Res. 1973 Aug;55(2):334–345. [PubMed] [Google Scholar]
  26. Wechsler J. A., Nüsslein V., Otto B., Klein A., Bonhoeffer F., Herrmann R., Gloger L., Schaller H. Isolation and characterization of thermosensitive Escherichia coli mutants defective in deoxyribonucleic acid replication. J Bacteriol. 1973 Mar;113(3):1381–1388. doi: 10.1128/jb.113.3.1381-1388.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Woodcock E., Grigg G. W. Repair of thermally induced DNA breakage in Escherichia coli. Nat New Biol. 1972 May 17;237(72):76–79. doi: 10.1038/newbio237076a0. [DOI] [PubMed] [Google Scholar]
  28. Youngs D. A., Bernstein I. A. Involvement of the recB-recC nuclease (exonuclease V) in the process of x-ray-induced deoxyribonucleic acid degradation in radiosensitive strains of Escherichia coli K-12. J Bacteriol. 1973 Feb;113(2):901–906. doi: 10.1128/jb.113.2.901-906.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Youngs D. A., Smith K. C. Involvement of DNA polymerase 3 in excision repair after ultraviolet irradiation. Nat New Biol. 1973 Aug 22;244(138):240–241. doi: 10.1038/newbio244240a0. [DOI] [PubMed] [Google Scholar]
  30. Youngs D. A., Smith K. C. Sensitivity to x-radiation of strains of Escherichia coli K-12 which lack DNA polymerase II. Mol Gen Genet. 1973 May 9;122(3):287–290. doi: 10.1007/BF00278604. [DOI] [PubMed] [Google Scholar]
  31. Youngs D. A., Smith K. C. X-ray sensitivity and repair capacity of a polA1 exrA strain of Escherichia coli K-12. J Bacteriol. 1973 Apr;114(1):121–127. doi: 10.1128/jb.114.1.121-127.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

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