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. 1976 Sep;127(3):1382–1388. doi: 10.1128/jb.127.3.1382-1388.1976

Membrane-associated, energy-linked reactions in Bdellovibrio bacteriovorus.

D Friedberg, I Friedberg
PMCID: PMC232934  PMID: 134028

Abstract

Disrupted cells of Bdellovibrio bacteriovorus exhibited adenosine triphosphatase activity, 60 to 80% of which was in the soluble fraction. Dicyclohexylcarbodiimide did not inhibit the adenosine triphosphatase activity in membrane particles. The particles did not show energy-linked transhydrogenase activity. The activity of non-energy-linked transhydrogenase as well as the rate of oxygen consumption were higher in membrane particles of the host-independent strain than in the host-dependent strains. The uptake of amino acid uptake was inhibited by cyanide and by carbonyl cyanide p-trifluoromethoxyphenyl hydrazone. Valinomycin, in the presence of K+, did not inhibit the uptake, and only partial inhibition was exerted by arsenate and dicyclohexylarbodiimide. Sulfhydryl reagents inhibited amino acid uptake.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abrams A., Smith J. B., Baron C. Carbodiimide-resistant membrane adenosine triphosphatase in mutants of Streptococcus faecalis. I. Studies of the mechanism of resistance. J Biol Chem. 1972 Mar 10;247(5):1484–1488. [PubMed] [Google Scholar]
  2. Berger E. A. Different mechanisms of energy coupling for the active transport of proline and glutamine in Escherichia coli. Proc Natl Acad Sci U S A. 1973 May;70(5):1514–1518. doi: 10.1073/pnas.70.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berger E. A., Heppel L. A. Different mechanisms of energy coupling for the shock-sensitive and shock-resistant amino acid permeases of Escherichia coli. J Biol Chem. 1974 Dec 25;249(24):7747–7755. [PubMed] [Google Scholar]
  4. Bulos B., Racker E. Partial resolution of the enzymes catalyzing oxidative phosphorylation. XVII. Further resolution of the rutamycin-sensitive adenosine triphosphatase. J Biol Chem. 1968 Jul 25;243(14):3891–3900. [PubMed] [Google Scholar]
  5. Fillingame R. H. Identification of the dicyclohexylcarbodiimide-reactive protein component of the adenosine 5'-triphosphate energy-transducing system of Escherichia coli. J Bacteriol. 1975 Nov;124(2):870–883. doi: 10.1128/jb.124.2.870-883.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fisher R. J., Lam K. W., Sanadi D. R. Escherichia coli nicotinamide adenine nucleotide transhydrogenase driven by aerobic oxidation and its enhancement by an energy transfer factor from rat-liver mitochondria. Biochem Biophys Res Commun. 1970;39(6):1021–1025. doi: 10.1016/0006-291x(70)90660-1. [DOI] [PubMed] [Google Scholar]
  7. Gadkari D., Stolp H. Energy metabolism of Bdellovibrio bacteriovorus. I. Energy production, ATP pool, energy charge. Arch Microbiol. 1975 Mar 10;102(3):179–185. doi: 10.1007/BF00428366. [DOI] [PubMed] [Google Scholar]
  8. Harold F. M., Baarda J. R., Baron C., Abrams A. Inhibition of membrane-bound adenosine triphosphatase and of cation transport in Streptococcus faecalis by N,N'-dicyclohexylcarbodiimide. J Biol Chem. 1969 May 10;244(9):2261–2268. [PubMed] [Google Scholar]
  9. Hespell R. B., Miozzari G. F., Rittenberg S. C. Ribonucleic acid destruction and synthesis during intraperiplasmic growth of Bdellovibrio bacteriovorus. J Bacteriol. 1975 Aug;123(2):481–491. doi: 10.1128/jb.123.2.481-491.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hespell R. B., Rosson R. A., Thomashow M. F., Rittenberg S. C. Respiration of Bdellovibrio bacteriovorus strain 109J and its energy substrates for intraperiplasmic growth. J Bacteriol. 1973 Mar;113(3):1280–1288. doi: 10.1128/jb.113.3.1280-1288.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kadner R. J., Winkler H. H. Energy coupling for methionine transport in Escherichia coli. J Bacteriol. 1975 Sep;123(3):985–991. doi: 10.1128/jb.123.3.985-991.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kanner B. I., Nelson N., Gutnick D. L. Differentiation between mutants of Escherichia coli K defective in oxidative phosphorylation. Biochim Biophys Acta. 1975 Sep 8;396(3):347–359. doi: 10.1016/0005-2728(75)90141-3. [DOI] [PubMed] [Google Scholar]
  13. Kuenen J. G., Rittenberg S. C. Incorporation of long-chain fatty acids of the substrate organism by Bdellovibrio bacteriovorus during intraperiplasmic growth. J Bacteriol. 1975 Mar;121(3):1145–1157. doi: 10.1128/jb.121.3.1145-1157.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Pritchard M. A., Langley D., Rittenberg S. Effects of methotrexate on intraperiplasmic and axenic growth of Bdellovibrio bacteriovorus. J Bacteriol. 1975 Mar;121(3):1131–1136. doi: 10.1128/jb.121.3.1131-1136.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rittenberg S. C., Hespell R. B. Energy efficiency of intraperiplasmic growth of Bdellovibrio bacteriovorus. J Bacteriol. 1975 Mar;121(3):1158–1165. doi: 10.1128/jb.121.3.1158-1165.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rittenberg S. C., Langley D. Utilization of nucleoside monophosphates per Se for intraperiplasmic growth of Bdellovibrio bacteriovorus. J Bacteriol. 1975 Mar;121(3):1137–1144. doi: 10.1128/jb.121.3.1137-1144.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rittenberg S. C., Shilo M. Early host damage in the infection cycle of Bdellovibrio bacteriovorus. J Bacteriol. 1970 Apr;102(1):149–160. doi: 10.1128/jb.102.1.149-160.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Seidler R. J., Starr M. P. Isolation and characterization of host-independent Bdellovibrios. J Bacteriol. 1969 Nov;100(2):769–785. doi: 10.1128/jb.100.2.769-785.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Simoni R. D., Postma P. W. The energetics of bacterial active transport. Annu Rev Biochem. 1975;44:523–554. doi: 10.1146/annurev.bi.44.070175.002515. [DOI] [PubMed] [Google Scholar]
  21. Simpson F. J., Robinson J. Some energy-producing systems in Bdellovibrio bacteriovorus, strain 6-5-S. Can J Biochem. 1968 Aug;46(8):865–873. doi: 10.1139/o68-129. [DOI] [PubMed] [Google Scholar]
  22. Singh A. P., Bragg P. D. Effect of dicyclohexylcarbodiimide on growth and membrane-mediated processes in wild type and heptose-deficient mutants of Escherichia coli K-12. J Bacteriol. 1974 Jul;119(1):129–137. doi: 10.1128/jb.119.1.129-137.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. West I., Mitchell P. Proton-coupled beta-galactoside translocation in non-metabolizing Escherichia coli. J Bioenerg. 1972 Aug;3(5):445–462. doi: 10.1007/BF01516082. [DOI] [PubMed] [Google Scholar]

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