Abstract
Polyphosphate of average chain length corresponding to 10 phosphate units was detected in the mycelial extract of zygomycetes. Gel electrophoresis techniques commonly used for the separation and characterization of acidic mucopolysaccharides were successfully used for the detection, purification, and characterization of the polyphosphate.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dietrich C. P., Dietrich S. M. Simple micro method for identification of heparin and other acidic mucopolysaccharides from mammalian tissues. Anal Biochem. 1972 Mar;46(1):209–215. doi: 10.1016/0003-2697(72)90413-7. [DOI] [PubMed] [Google Scholar]
- Dietrich C. P., Nader H. B., Britto L. R., Silva M. E. Chemical composition of heparitin sulfate. Fractionation and characterization of four acidic mucopolysaccharides in heparitin sulfate from beef lung tissue. Biochim Biophys Acta. 1971 Jun 22;237(3):430–441. doi: 10.1016/0304-4165(71)90260-1. [DOI] [PubMed] [Google Scholar]
- Felter S., Dirheimer G., Ebel J. P. Les polyphosphatases de levure de boulangerie. Bull Soc Chim Biol (Paris) 1970;52(4):433–446. [PubMed] [Google Scholar]
- HAROLD F. M. Accumulation of inorganic polyphosphate in mutants of Neurospora crassa. Biochim Biophys Acta. 1960 Dec 4;45:172–188. doi: 10.1016/0006-3002(60)91438-4. [DOI] [PubMed] [Google Scholar]
- Harold F. M. Inorganic polyphosphates in biology: structure, metabolism, and function. Bacteriol Rev. 1966 Dec;30(4):772–794. doi: 10.1128/br.30.4.772-794.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hilborn J. C., Anastassiadis P. A. Estimation of the molecular weights of acidic mucopolysaccharides by polyacrylamide gel electrophoresis. Anal Biochem. 1971 Jan;39(1):88–92. doi: 10.1016/0003-2697(71)90465-9. [DOI] [PubMed] [Google Scholar]
- Jaques L. B., Ballieux R. E., Dietrich C. P., Kavanagh L. W. A microelectrophoresis method for heparin. Can J Physiol Pharmacol. 1968 May;46(3):351–360. doi: 10.1139/y68-055. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Wang M. C., Bartnicki-Garcia S. Novel phosphoglucans from the cytoplasm of Phytophthora palmivora and their selective occurrence in certain life cycle stages. J Biol Chem. 1973 Jun 10;248(11):4112–4118. [PubMed] [Google Scholar]