Abstract
After the addition of glutaraldehyde (GA) to cells incubated at 3 or 37 degrees C, mesosomes were observed with increasing frequencies in freeze fractures of cells. These increases were related to the kinetics with which GA cross-linked adjacent amino acids. Upon the addition of GA, mesosomes were first observed in the periphery of freeze-fractured cells usually attached to septal membranes. However, the time, while the septal attachment sites were maintained, the "bodies" of the mesosomes were observed to move toward the center of the cytoplasm. This centralization process was much more rapid at 37 than at 3 degrees C. It is hypothesized that upon fixation, or receipt of some physical insult, mesosome precursors found in undisturbed cells undergo a change in state that results in their visibility in freeze fractures.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Higgins M. L., Daneo-Moore L. Factors influencing the frequency of mesosomes observed in fixed and unfixed cells of Streptococcus faecalis. J Cell Biol. 1974 May;61(2):288–300. doi: 10.1083/jcb.61.2.288. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Higgins M. L., Daneo-Moore L. Morphokinetic reaction of cells of Streptococcus faecalis (ATCC 9790) to specific inhibition of macromolecular synthesis: dependence of mesosome growth on deoxyribonucleic acid synthesis. J Bacteriol. 1972 Mar;109(3):1221–1231. doi: 10.1128/jb.109.3.1221-1231.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Higgins M. L., Pooley H. M., Shockman G. D. Reinitiation of cell wall growth after threonine starvation of Streptococcus faecalis. J Bacteriol. 1971 Mar;105(3):1175–1183. doi: 10.1128/jb.105.3.1175-1183.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Higgins M. L., Shockman G. D. Early changes in the ultrastructure of Streptococcus faecalis after amino acid starvation. J Bacteriol. 1970 Jul;103(1):244–253. doi: 10.1128/jb.103.1.244-253.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Higgins M. L., Shockman G. D. Procaryotic cell division with respect to wall and membranes. CRC Crit Rev Microbiol. 1971 May;1(1):29–72. doi: 10.3109/10408417109104477. [DOI] [PubMed] [Google Scholar]
- Nanninga N. The mesosome of Bacillus subtilis as affected by chemical and physical fixation. J Cell Biol. 1971 Jan;48(1):219–224. doi: 10.1083/jcb.48.1.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roth G. S., Shockman G. D., Daneo-Moore L. Balanced macromolecular biosynthesis in "protoplasts" of Streptococcus faecalis. J Bacteriol. 1971 Mar;105(3):710–717. doi: 10.1128/jb.105.3.710-717.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Salton M. R. Bacterial membranes. CRC Crit Rev Microbiol. 1971 May;1(1):161–197. doi: 10.3109/10408417109104480. [DOI] [PubMed] [Google Scholar]
- Tsien H. C., Higgins M. L. Effect of temperature on the distribution of membrane particles in Streptococcus faecalis as seen by the freeze-fracture technique. J Bacteriol. 1974 May;118(2):725–734. doi: 10.1128/jb.118.2.725-734.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]