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. 1976 Aug;127(2):837–847. doi: 10.1128/jb.127.2.837-847.1976

Deoxyribonucleic acid-binding studies on the hut repressor and mutant forms of the hut repressor of Salmonella typhimurium.

D C Hagen, B Magasanik
PMCID: PMC232992  PMID: 8423

Abstract

In Salmonella typhimurium the genes coding for the enzymes of histidine utilization (hut) are clustered in two adjacent operons, hutMIGC and hut(P,R,Q)UH. A single repressor, the product of the C gene, regulates both operons by binding at two operator sites, one near M and one in (P,R,Q). The deoxyribonucleic acid (DNA)-binding activity of the repressor was measured using DNA's containing separate operators. The repressor had greater activity when assayed using DNA containing the operator of the (P,R,Q)UH operon than when assayed using DNA containing the operator of the MIGC operon. The binding to either operator was absent in the presence of the inducer, urocanate. The DNA-binding activities were also determined for two super-repressors. The super-repressors had altered DNA-binding properties, although the self-regulated nature of the repressors complicated the analysis of the results. A purfication procedure for the wild-type repressor is presented. The purified repressor was somewhat unstable, and additional experiments using it were not performed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brill W. J., Magasanik B. Genetic and metabolic control of histidase and urocanase in Salmonella typhimurium, strain 15-59. J Biol Chem. 1969 Oct 10;244(19):5392–5402. [PubMed] [Google Scholar]
  2. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hagen D. C., Gerson S. L., Magasanik B. Isolation of super-repressor mutants in the histidine utilization system of Salmonella typhimurium. J Bacteriol. 1975 Feb;121(2):583–593. doi: 10.1128/jb.121.2.583-593.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hagen D. C., Lipton P. J., Magasanik B. Isolation of a trans-dominant histidase-negative mutant of Salmonella typhimurium. J Bacteriol. 1974 Nov;120(2):906–916. doi: 10.1128/jb.120.2.906-916.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hagen D. C., Magasanik B. Isolation of the self-regulated repressor protein of the Hut operons of Salmonella typhimurium. Proc Natl Acad Sci U S A. 1973 Mar;70(3):808–812. doi: 10.1073/pnas.70.3.808. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jobe A., Riggs A. D., Bourgeois S. Lac repressor-operator interaction. V. Characterization of super- and pseudo-wild-type repressors. J Mol Biol. 1972 Feb 28;64(1):181–199. doi: 10.1016/0022-2836(72)90328-2. [DOI] [PubMed] [Google Scholar]
  7. KAISER A. D. Mutations in a temperate bacteriophage affecting its ability to lysogenize Escherichia coli. Virology. 1957 Feb;3(1):42–61. doi: 10.1016/0042-6822(57)90022-3. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Meiss H. K., Brill W. J., Magasanik B. Genetic control of histidine degradation in Salmonella typhimurium, strain LT-2. J Biol Chem. 1969 Oct 10;244(19):5382–5391. [PubMed] [Google Scholar]
  10. Parada J. L., Magasanik B. Expression of the hut operons of Salmonella typhimurium in Klebsiella aerogenes and in Escherichia coli. J Bacteriol. 1975 Dec;124(3):1263–1268. doi: 10.1128/jb.124.3.1263-1268.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Riggs A. D., Suzuki H., Bourgeois S. Lac repressor-operator interaction. I. Equilibrium studies. J Mol Biol. 1970 Feb 28;48(1):67–83. doi: 10.1016/0022-2836(70)90219-6. [DOI] [PubMed] [Google Scholar]
  12. Sanderson K. E. Linkage map of Salmonella typhimurium, edition IV. Bacteriol Rev. 1972 Dec;36(4):558–586. doi: 10.1128/br.36.4.558-586.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shapiro A. L., Viñuela E., Maizel J. V., Jr Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815–820. doi: 10.1016/0006-291x(67)90391-9. [DOI] [PubMed] [Google Scholar]
  14. Smith G. R., Halpern Y. S., Magasanik B. Genetic and metabolic control of enzymes responsible for histidine degradation in Salmonella typhimurium. 4-imidazolone-5-propionate amidohydrolase and N-formimino-L-glutamate formiminohydrolase. J Biol Chem. 1971 May 25;246(10):3320–3329. [PubMed] [Google Scholar]
  15. Smith G. R., Magasanik B. Nature and self-regulated synthesis of the repressor of the hut operons in Salmonella typhimurium. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1493–1497. doi: 10.1073/pnas.68.7.1493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Smith G. R., Magasanik B. The two operons of the histidine utilization system in Salmonella typhimurium. J Biol Chem. 1971 May 25;246(10):3330–3341. [PubMed] [Google Scholar]
  17. Smith G. R. Specialized transduction of the Salmonella hut operons by coliphage lambda: deletion analysis of the hut operons employing lambda-phut. Virology. 1971 Jul;45(1):208–223. doi: 10.1016/0042-6822(71)90128-0. [DOI] [PubMed] [Google Scholar]
  18. Studier F. W., Maizel J. V., Jr T7-directed protein synthesis. Virology. 1969 Nov;39(3):575–586. doi: 10.1016/0042-6822(69)90105-6. [DOI] [PubMed] [Google Scholar]
  19. Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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