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. 1976 Jul;127(1):327–333. doi: 10.1128/jb.127.1.327-333.1976

Similarity in properties and mapping of three Rec mutants of Haemophilus influenzae.

J Kooistra, J K Setlow
PMCID: PMC233065  PMID: 1084339

Abstract

Three Rec- mutants of Haemophilus influenzae have been studied with respect to their transformability, ultraviolet and mitomycin C sensitivities, spontaneous and ultraviolet-induced deoxyribonucleic acid breakdown, inducibility of lysogens, and the linkage of the three mutations to a streptomycin resistance marker. The data indicate that the three mutations cause the same phenotypic changes, and that they are all on the same gene. Transformability of the mutants is different when two different media are used for competence development, although transformability with the two competence methods is not different in a Rec- strain that is mutant at another gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beattie K. L., Setlow J. K. Transformation-defective strains of Haemophilus influenzae. Nat New Biol. 1971 Jun 9;231(23):177–179. doi: 10.1038/newbio231177a0. [DOI] [PubMed] [Google Scholar]
  2. Boling M. E., Setlow J. K. Dependence of Vegetative Recombination Among Haemophilus influenzae Bacteriophage on the Host Cell. J Virol. 1969 Sep;4(3):240–243. doi: 10.1128/jvi.4.3.240-243.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Caster J. H., Postel E. H., Goodgal S. H. Competence mutants: isolation of transformation deficient strains of Haemophilus influenzae. Nature. 1970 Aug 1;227(5257):515–517. doi: 10.1038/227515a0. [DOI] [PubMed] [Google Scholar]
  4. Clark A. J., Chamberlin M., Boyce R. P., Howard-Flanders P. Abnormal metabolic response to ultraviolet light of a recombination deficient mutant of Escherichia coli K12. J Mol Biol. 1966 Aug;19(2):442–454. doi: 10.1016/s0022-2836(66)80015-3. [DOI] [PubMed] [Google Scholar]
  5. Clark A. J. Recombination deficient mutants of E. coli and other bacteria. Annu Rev Genet. 1973;7:67–86. doi: 10.1146/annurev.ge.07.120173.000435. [DOI] [PubMed] [Google Scholar]
  6. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Herriott R. M., Meyer E. Y., Vogt M., Modan M. Defined medium for growth of Haemophilus influenzae. J Bacteriol. 1970 Feb;101(2):513–516. doi: 10.1128/jb.101.2.513-516.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Howard-Flanders P., Boyce R. P. DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes. Radiat Res. 1966;(Suppl):156+–156+. [PubMed] [Google Scholar]
  9. Howard-Flanders P. DNA repair. Annu Rev Biochem. 1968;37:175–200. doi: 10.1146/annurev.bi.37.070168.001135. [DOI] [PubMed] [Google Scholar]
  10. Kooistra J., Venema G. Fate of donor DNA in some poorly transformable strains of Haemophilus influenzae. Mutat Res. 1970 Mar;9(3):245–253. doi: 10.1016/0027-5107(70)90126-0. [DOI] [PubMed] [Google Scholar]
  11. Kooistra J., Venema G. Fate of donor deoxyribonucleic acid in a highly transformation-deficient strain of Haemophilus influenzae. J Bacteriol. 1974 Sep;119(3):705–717. doi: 10.1128/jb.119.3.705-717.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Leclerc J. E., Setlow J. K. Postreplication repair of ultraviolet damage in Haemophilus influenzae. J Bacteriol. 1972 Jun;110(3):930–934. doi: 10.1128/jb.110.3.930-934.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Muhammed A., Setlow J. K. Ultraviolet-induced decrease in integration of Haemophilus influenzae transforming deoxyribonucleic acid in sensitive and resistant cells. J Bacteriol. 1970 Feb;101(2):444–448. doi: 10.1128/jb.101.2.444-448.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Notani N. K., Setlow J. K., Joshi V. R., Allison D. P. Molecular basis for the transformation defects in mutants of Haemophilus influenzae. J Bacteriol. 1972 Jun;110(3):1171–1180. doi: 10.1128/jb.110.3.1171-1180.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. STUY J. H. Studies on the radiation inactivation of microorganisms. VI. X-ray induced breakdown of deoxyribonucleic acid in Haemophilus influenzae and in other bacteria. J Bacteriol. 1960 May;79:707–715. doi: 10.1128/jb.79.5.707-715.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Setlow J. K., Boling M. E., Beattie K. L., Kimball R. F. A complex of recombination and repair genes in Haemophilus influenzae. J Mol Biol. 1972 Jul 21;68(2):361–378. doi: 10.1016/0022-2836(72)90218-5. [DOI] [PubMed] [Google Scholar]
  17. Setlow J. K., Brown D. C., Boling M. E., Mattingly A., Gordon M. P. Repair of deoxyribonucleic acid in Haemophilus influenzae. I. X-ray sensitivity of ultraviolet-sensitive mutants and their behavior as hosts to ultraviolet-irradiated bacteriophage and transforming deoxyribonucleic acid. J Bacteriol. 1968 Feb;95(2):546–558. doi: 10.1128/jb.95.2.546-558.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Small G. D., Setlow J. K., Kooistra J., Shapanka R. Lethal effect of mitomycin C on Haemophilus influenzae. J Bacteriol. 1976 Feb;125(2):643–654. doi: 10.1128/jb.125.2.643-654.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Steinhart W. L., Herriott R. M. Fate of recipient deoxyribonucleic acid during transformation in Haemophilus influenzae. J Bacteriol. 1968 Nov;96(5):1718–1724. doi: 10.1128/jb.96.5.1718-1724.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Willetts N. S., Clark A. J. Characteristics of some multiply recombination-deficient strains of Escherichia coli. J Bacteriol. 1969 Oct;100(1):231–239. doi: 10.1128/jb.100.1.231-239.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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