Abstract
Standard laboratory yeast strains can be enriched for thymidine 5'-monophosphate (TMP) uptake derivatives that generate only a low percentage of respiratory-deficient colonies (petites) under inhibition of TMP biosynthesis. Such mutants incorporated bromodeoxyuridine 5'-monophosphate (BrdUMP) into both nuclear and mitochondrial deoxyribonucleic acid (mtDNA); however, they showed a selectivity for TMP over BrdUMP incorporation. The preferential incorporation of [3H]TMP or BrdUMP into mtDNA was strain dependent. The density increments after growth in the presence of BrdUMP reached 50 mg/ml for nuclear DNA and 22 mg/ml for mtDNA in CsCl gradients. Density shifts corresponding to 4% bromouracil substitution were easily detected. Preliminary density transfer experiments confirm that mtDNA does not replicate in synchrony with nuclear DNA.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brendel M., Fäth W. W., Laskowski W. Isolation and characterization of mutants of Saccharomyces cerevisiae able to grow after inhibition of dTMP synthesis. Methods Cell Biol. 1975;11:287–294. doi: 10.1016/s0091-679x(08)60329-5. [DOI] [PubMed] [Google Scholar]
- Brendel M., Haynes R. H. Kinetics and genetic control of the incorporation of thymidine monophosphate in yeast DNA. Mol Gen Genet. 1972;117(1):39–44. doi: 10.1007/BF00268835. [DOI] [PubMed] [Google Scholar]
- Corneo G., Moore C., Sanadi D. R., Grossman L. I., Marmur J. Mitochondrial DNA in yeast and some mammalian species. Science. 1966 Feb 11;151(3711):687–689. doi: 10.1126/science.151.3711.687. [DOI] [PubMed] [Google Scholar]
- Flory P. J., Jr, Vinograd J. 5-bromodeoxyuridine labeling of monomeric and catenated circular mitochondrial DNA in HeLa cells. J Mol Biol. 1973 Feb 25;74(2):81–94. doi: 10.1016/0022-2836(73)90100-9. [DOI] [PubMed] [Google Scholar]
- Fäth W. W., Brendel M., Laskowski W., Lehmann-Brauns E. Economizing DNA-specific labelling by deoxythymidine-5'-monophosphate in Saccharomyces cerevisiae. Mol Gen Genet. 1974;132(4):335–345. doi: 10.1007/BF00268573. [DOI] [PubMed] [Google Scholar]
- Fäth W. W., Brendel M. Specific DNA-labelling by exogenous thymidine-5'-monophosphate in Saccharomyces cerevisiae. Mol Gen Genet. 1974;131(1):57–67. doi: 10.1007/BF00269387. [DOI] [PubMed] [Google Scholar]
- Goldthwaite C. D., Cryer D. R., Marmur J. Effect of carbon source on the replication and transmission of yeast mitochondrial genomes. Mol Gen Genet. 1974;133(2):87–104. doi: 10.1007/BF00264830. [DOI] [PubMed] [Google Scholar]
- Grivell A. R., Jackson J. F. Thymidine kinase: evidence for its absence from Neurospora crassa and some other micro-organisms, and the relevance of this to the specific labelling of deoxyribonucleic acid. J Gen Microbiol. 1968 Dec;54(2):307–317. doi: 10.1099/00221287-54-2-307. [DOI] [PubMed] [Google Scholar]
- Gross N. J., Rabinowitz M. Synthesis of new strands of mitochondrial and nuclear deoxyribonucleic acid by semiconservative replication. J Biol Chem. 1969 Mar 25;244(6):1563–1566. [PubMed] [Google Scholar]
- Hackett P., Jr, Hanawalt P. Selectivity for thymine over 5-bromouracil by a thymine-requiring bacterium. Biochim Biophys Acta. 1966 Aug 17;123(2):356–363. doi: 10.1016/0005-2787(66)90288-7. [DOI] [PubMed] [Google Scholar]
- Hartwell L. H., Culotti J., Reid B. Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A. 1970 Jun;66(2):352–359. doi: 10.1073/pnas.66.2.352. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hutchinson F. The lesions produced by ultraviolet light in DNA containing 5-bromouracil. Q Rev Biophys. 1973 May;6(2):201–246. doi: 10.1017/s0033583500001141. [DOI] [PubMed] [Google Scholar]
- Jannsen S., Witte I., Megnet R. Mutants for the specific labelling of DNA in Saccharomyces cerevisiae. Biochim Biophys Acta. 1973 Apr 11;299(4):681–685. doi: 10.1016/0005-2787(73)90243-8. [DOI] [PubMed] [Google Scholar]
- MICHELSON A. M., DONDON J., GRUNBERG-MANAGO M. The action of polynucleotide phosphorylase on 5-halogenouridine-5' pyrophosphates. Biochim Biophys Acta. 1962 Apr 2;55:529–540. doi: 10.1016/0006-3002(62)90986-1. [DOI] [PubMed] [Google Scholar]
- Meselson M., Stahl F. W. THE REPLICATION OF DNA IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1958 Jul 15;44(7):671–682. doi: 10.1073/pnas.44.7.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Petes T. D., Fangman W. L. Preferential synthesis of yeast mitochondrial DNA in alpha factor-arrested cells. Biochem Biophys Res Commun. 1973 Dec 10;55(3):603–609. doi: 10.1016/0006-291x(73)91186-8. [DOI] [PubMed] [Google Scholar]
- Wickner R. B. Mutants of Saccharomyces cerevisiae that incorporate deoxythymidine 5'-monophosphate into DNA in vivo. Methods Cell Biol. 1975;11:295–302. doi: 10.1016/s0091-679x(08)60330-1. [DOI] [PubMed] [Google Scholar]
- Wickner R. B. Mutants of Saccharomyces cerevisiae that incorporate deoxythymidine-5'-monophosphate into deoxyribonucleic acid in vivo. J Bacteriol. 1974 Jan;117(1):252–260. doi: 10.1128/jb.117.1.252-260.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Williamson D. H., Fennell D. J. Apparent dispersive replication of yeast mitochondrial DNA as revealed by density labelling experiments. Mol Gen Genet. 1974;131(3):193–207. doi: 10.1007/BF00267959. [DOI] [PubMed] [Google Scholar]