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. 1976 Jul;127(1):406–417. doi: 10.1128/jb.127.1.406-417.1976

Regulation of beta-glucuronidase synthesis in Escherichia coli K-12: constitutive mutants specifically derepressed for uidA expression.

M Novel, G Novel
PMCID: PMC233074  PMID: 776933

Abstract

All methyl-beta-D-galacturonide-positive mutants isolated from Escherichia coli K-12 carry constitutive mutations for beta-glucuronidase (UID) synthesis. Most of these mutants are specific for UID synthesis and are distributed in three classes according to the derepression level of UID. Each specific mutant carries a mutation(s) near uidA, the structural gene for UID, at min 30.5 of the E. coli K-12 linkage map. The expression of UID synthesis in F-merodiploid strains carrying these mutations permits discrimination between dominant and recessive constitutivity over the wild-type allele. The first kind of mutation (dominant) should affect the operator site uidO of the structural gene uidA; the second type of mutation (recessive) should affect a regulatory gene, uidR, operating through a negative control. The isolation of mutants bearing at this locus superrepressed mutations, which can revert to produce a constitutive phenotype, confirms the occurrence of such a regulatory gene. The partially derepressed uidR mutants of the first class are normally inducible and remain constitutive at low temperature; their UID has the same thermal sensitivity as in the wild-type strains. The occurrence of similar regulatory gene mutants has been recently described in the lactose system (Shineberg, 1974).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baudouy-Robert J., Didier-Fichet M. L., Jimeno-Abendano J., Novel G., Portalier R., Stoeber F. Modalités de l'induction des six premières enzymes dégradant les hexuronides et les hexuronates chez Escherichia coli K 12. C R Acad Sci Hebd Seances Acad Sci D. 1970 Jul 15;271(2):255–258. [PubMed] [Google Scholar]
  2. Didier-Fichet M. L., Stoeber F. Sur les activités glucuronidasique et galacturonidasique d'Escherichia coli ML 30. C R Acad Sci Hebd Seances Acad Sci D. 1968 Apr 29;266(18):1894–1897. [PubMed] [Google Scholar]
  3. Didier-Fichet M. L., Stoeber F. Sur les propriétés et la biosynthèse de la beta-glucuronidase d'Escherichia coli K 12. C R Acad Sci Hebd Seances Acad Sci D. 1968 May 6;266(19):2021–2024. [PubMed] [Google Scholar]
  4. JACOB F., MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318–356. doi: 10.1016/s0022-2836(61)80072-7. [DOI] [PubMed] [Google Scholar]
  5. Jayaraman K., Müller-Hill B., Rickenberg H. V. Inhibition of the synthesis of beta-galactosidase in Escherichia coli by 2-nitrophenyl-beta-D-fucoside. J Mol Biol. 1966 Jul;18(2):339–343. doi: 10.1016/s0022-2836(66)80251-6. [DOI] [PubMed] [Google Scholar]
  6. Low B. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc Natl Acad Sci U S A. 1968 May;60(1):160–167. doi: 10.1073/pnas.60.1.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Low K. B. Escherichia coli K-12 F-prime factors, old and new. Bacteriol Rev. 1972 Dec;36(4):587–607. doi: 10.1128/br.36.4.587-607.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Novel G., Didier-Fichet M. L., Stoeber F. Inducibility of beta-glucuronidase in wild-type and hexuronate-negative mutants of Escherichia coli K-12. J Bacteriol. 1974 Oct;120(1):89–95. doi: 10.1128/jb.120.1.89-95.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Novel G., Novel M., Didier-Fichet M. L., Stoeber F. Etude génétique de mutants du système de dégradation des hexuronides chez Escherichia coli K 12. C R Acad Sci Hebd Seances Acad Sci D. 1970 Jul 27;271(4):457–460. [PubMed] [Google Scholar]
  10. Novel G., Novel M. Mutants d'Escherichia coli K 12 affectés pour leur croissance sur méthyl-beta-D-glucuronide: localisation of gène de structure de la beta-D-glucuronidase (uid A. Mol Gen Genet. 1973;120(4):319–335. [PubMed] [Google Scholar]
  11. Novel M., Novel G. Mutants d'Escherichia coli K-12, capables de croître sur méthyl-beta-D-galacturonide: mutants simples constitutifs pour la synthèse de la beta-glucuronidase et mutants doubles déréprimés aussi pour la synthèse de deux enzymes d'utilisation du glucuronate. C R Acad Sci Hebd Seances Acad Sci D. 1974 Aug 19;279(8):695–698. [PubMed] [Google Scholar]
  12. Novel M., Novel G. Regulation of beta-glucuronidase synthesis in Escherichia coli K-12: pleiotropic constitutive mutations affecting uxu and uidA expression. J Bacteriol. 1976 Jul;127(1):418–432. doi: 10.1128/jb.127.1.418-432.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Portalier R. C., Stoeber F. R. Dosages colorimétriques des oxydoréductases aldoniques d'Escherichia coli K 12: applications. Biochim Biophys Acta. 1972 Nov 10;289(1):19–27. doi: 10.1016/0005-2744(72)90103-9. [DOI] [PubMed] [Google Scholar]
  14. Robert-Baudouy J. M., Portalier R. C. Mutations affectant le catabolisme du glucuronate chez Escherichia coli K12. Mol Gen Genet. 1974;131(1):31–46. doi: 10.1007/BF00269385. [DOI] [PubMed] [Google Scholar]
  15. Robert-Baudouy J. M., Portalier R. C., Stoeber F. R. Régulation due métabolisme des hexuronates chez Escherichia coli K12. Modalités de l'induction des enzymes du système hexuronate. Eur J Biochem. 1974 Mar 15;43(1):1–15. doi: 10.1111/j.1432-1033.1974.tb03378.x. [DOI] [PubMed] [Google Scholar]
  16. SISTROM W. R. On the physical state of the intracellularly accumulates substrates of beta-galactoside-permease in Escherichia coli. Biochim Biophys Acta. 1958 Sep;29(3):579–587. doi: 10.1016/0006-3002(58)90015-5. [DOI] [PubMed] [Google Scholar]
  17. STACEY K. A., SIMSON E. IMPROVED METHOD FOR THE ISOLATION OF THYMINE-REQUIRING MUTANTS OF ESCHERICHIA COLI. J Bacteriol. 1965 Aug;90:554–555. doi: 10.1128/jb.90.2.554-555.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Saedler H., Gullon A., Fiethen L., Starlinger P. Negative control of the galactose operon in E. coli. Mol Gen Genet. 1968;102(1):79–88. doi: 10.1007/BF00341872. [DOI] [PubMed] [Google Scholar]
  19. Shineberg B. Mutations partially inactivating the lactose repressor of Escherichia coli. J Bacteriol. 1974 Aug;119(2):500–507. doi: 10.1128/jb.119.2.500-507.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stoeber F., Lagarde A., Nemoz G., Novel G., Novel M., Portalier R., Pouyssegur J., Robert-Baudouy J. Le métabolisme des hexuronides et des hexuronates chez Escherichia coli K 12: aspects physiologiques et et génétiques de sa régulation. Biochimie. 1974;56(2):199–213. doi: 10.1016/s0300-9084(74)80379-2. [DOI] [PubMed] [Google Scholar]
  21. Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. WILLSON C., PERRIN D., COHN M., JACOB F., MONOD J. NON-INDUCIBLE MUTANTS OF THE REGULATOR GENE IN THE "LACTOSE" SYSTEM OF ESCHERICHIA COLI. J Mol Biol. 1964 Apr;8:582–592. doi: 10.1016/s0022-2836(64)80013-9. [DOI] [PubMed] [Google Scholar]

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