Abstract
The ultrastructure of Acinetobacter sp. strain HO1-N grown on hydrocarbon and nonhydrocarbon substrates was compared using thin sections and freeze-etching. Hydrocarbon-grown cells were characterized by the presence of intracytoplasmic membrane-bound hexadecane inclusions. This membrane did not exhibit a typical unit membrane structure but appeared as a monolayer. The freeze-etch technique revealed the internal structure of the hexadecane inclusions and provided evidence for the presence of a smooth-surfaced limiting membrane. Freeze-etching also revealed intracytoplasmic membranes in the hexadecane-grown cells. These ultrastructural modifications were not present in nonhydrocarbon-grown cells. The hexadecane inclusions were isolated from Acinetobacter. Negative-staining of the inclusions revealed electron-transparent vesicles approximating the size of the inclusions seen in whole cells. Freeze-etching of the purified inclusions revealed membrane-bound vesicles. The purified inclusions exhibited a relatively high value of lipid phosphorus to protein. The lipid composition and the electrophoretic banding pattern of the inclusions on sodium dodecyl sulfate-polyacrylamide gels were determined and compared with other membrane fractions (outer membrane and cytoplasmic membrane) previously isolated from this organism.
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- BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
- Dunlop W. F., Robards A. W. Ultrastructural study of poly- -hydroxybutyrate granules from Bacillus cereus. J Bacteriol. 1973 Jun;114(3):1271–1280. doi: 10.1128/jb.114.3.1271-1280.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kennedy R. S., Finnerty W. R. Microbial assimilation of hydrocarbons. I. The fine-structure of a hydrocarbon oxidizing Acinetobacter sp. Arch Microbiol. 1975;102(2):75–83. doi: 10.1007/BF00428349. [DOI] [PubMed] [Google Scholar]
- Kennedy R. S., Finnerty W. R. Microbial assimilation of hydrocarbons. II. Intracytoplasmic membrane induction in Acinetobacter sp. Arch Microbiol. 1975;102(2):85–90. doi: 10.1007/BF00428350. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Ludvík J., Munk V., Dostálek M. Ultrastructural changes in the yeast Candida lipolytica caused by penetration of hydrocarbons into the cell. Experientia. 1968 Oct 15;24(10):1066–1068. doi: 10.1007/BF02138754. [DOI] [PubMed] [Google Scholar]
- PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STAEUBLI W. A new embedding technique for electron microscopy, combining a water-soluble epoxy resin (Durcupan) with water-insoluble Araldite. J Cell Biol. 1963 Jan;16:197–201. doi: 10.1083/jcb.16.1.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scott C. C., Makula S. R., Finnerty W. R. Isolation and characterization of membranes from a hydrocarbon-oxidizing Acinetobacter sp. J Bacteriol. 1976 Jul;127(1):469–480. doi: 10.1128/jb.127.1.469-480.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shively J. M. Inclusion bodies of prokaryotes. Annu Rev Microbiol. 1974;28(0):167–187. doi: 10.1146/annurev.mi.28.100174.001123. [DOI] [PubMed] [Google Scholar]
- Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969 Jan;26(1):31–43. doi: 10.1016/s0022-5320(69)90033-1. [DOI] [PubMed] [Google Scholar]