Abstract
In Anacystis nidulans, upon infection with cyanophage AS-1, after a lag period of 1 h the level of deoxyribonuclease (DNase) activity increaded rapidly up to 15- to 20-fold in 4 to 5 h in the light. In contrast, the ribonuclease and phosphomonoesterase activities increased significantly only 4 to 5 h after infection, i.e. as late as 1 h prior to lysis. In complete darkness, the nuclease levels remained unaltered. However, when the infected cells were exposed to light for 1 or 2 h after infection, the DNase level increased essentially to the same extent in the dark as in continuous light, although the complete replication cycle of the virus was impaired in the dark and cells lysed only in the continuously illuminated cultures. Inhibition of photosystem II with 3-(3,4-dichlorophenyl)-1-dimethylurea during the early illumination period strongly decreased the subsequent, infection-dependent increase in DNase activity in the dark. The virus-induced increase in DNase activity was also inhibited by chloramphenicol. The data suggest that, in spite of the obligate photoautotrophic nature of A. nidulans, dark metabolism is able to support fully the formation of some specific proteins if the triggering of their synthesis takes place in light.
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Selected References
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- Doolittle W. F., Singer R. A. Mutational analysis of dark endogenous metabolism in the blue-green bacterium Anacystis nidulans. J Bacteriol. 1974 Sep;119(3):677–683. doi: 10.1128/jb.119.3.677-683.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mann N., Carr N. G. Control of macromolecular composition and cell division in the blue-green algae Anacystis nidulans. J Gen Microbiol. 1974 Aug;83(2):399–405. doi: 10.1099/00221287-83-2-399. [DOI] [PubMed] [Google Scholar]
- Norton J., Roth J. S. Some aspects of nuclease activity in Anacystis nidulans and other algae. Comp Biochem Physiol. 1967 Nov;23(2):361–371. doi: 10.1016/0010-406x(67)90392-1. [DOI] [PubMed] [Google Scholar]
- Pelroy R. A., Rippka R., Stanier R. Y. Metabolism of glucose by unicellular blue-green algae. Arch Mikrobiol. 1972;87(4):303–322. doi: 10.1007/BF00409131. [DOI] [PubMed] [Google Scholar]
- Safferman R. S., Diener T. O., Desjardins P. R., Morris M. E. Isolation and characterization of AS-1, a phycovirus infecting the blue-green algae, Anacystis nidulans and Synechococcus cedrorum. Virology. 1972 Jan;47(1):105–113. doi: 10.1016/0042-6822(72)90243-7. [DOI] [PubMed] [Google Scholar]
- Singer R. A., Doolittle W. F. Control of gene expression in blue-green algae. Nature. 1975 Feb 20;253(5493):650–651. doi: 10.1038/253650a0. [DOI] [PubMed] [Google Scholar]
- Udvardy J., Marrè E., Farkas G. L. Purification and properties of a phosphodiesterase from Avena leaf tissues. Biochim Biophys Acta. 1970 Jun 10;206(3):392–403. doi: 10.1016/0005-2744(70)90155-5. [DOI] [PubMed] [Google Scholar]
- Wyen N. V., Erdei S., Farkas G. L. Isolation from Avena leaf tissues of a nuclease with the same type of specificity towards RNA and DNA. Accumulation of the enzyme during leaf senescence. Biochim Biophys Acta. 1971 Mar 25;232(3):472–483. doi: 10.1016/0005-2787(71)90601-0. [DOI] [PubMed] [Google Scholar]
- Wyen N. V., Udvardy J., Erdei S., Farkas G. L. The level of a relatively purine-specific ribonuclease increases in virus-infected hypersensitive or mechanically injured tobacco leaves. Virology. 1972 May;48(2):337–341. doi: 10.1016/0042-6822(72)90044-x. [DOI] [PubMed] [Google Scholar]