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. 1976 May;126(2):751–757. doi: 10.1128/jb.126.2.751-757.1976

Fatty acid transport by the lipophilic bacterium Nocardia asteroides.

R Calmes, S J Deal
PMCID: PMC233210  PMID: 770452

Abstract

Hexadecanoate was translocated in Nocardia asteroides by a constitutive transport system(s), which transported short, medium, and long-chain fatty acids. Inhibition of hexadenocanoate transport by homologues suggested that at least two systems are present: one specific for short-chain fatty acids and the other specific for medium- and long-chain fatty acids. Saturation kinetics typical of a carrier-mediated transport system (Kt = 870 muM)were observed, and concentration of fatty acids against a gradient was achieved. Inhibitor studies indicated that free sulfhydryl groups, a functional respiratory chain, and energy are required for translocation. Efflux of [14C]hexadecanoate in the presence of excess unlabeled hexadecanoate or 2,4-dinitrophenol and the cytoplasmic localization of acyl-coenzyme A synthetase (acid:coenzyme A ligase [adenosine monophosphate]; EC 6.2.1.3) (Calmes and Deal, 1973) are consistent with the hypothesis that fatty acids are transported and released intracellularly as free fatty acids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnes E. M., Jr, Kaback H. R. Beta-galactoside transport in bacterial membrane preparations: energy coupling via membrane-bounded D-lactic dehydrogenase. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1190–1198. doi: 10.1073/pnas.66.4.1190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Calmes R., Deal S. J. Fatty acid activation by a lipophilic bacterium. J Bacteriol. 1973 Apr;114(1):249–256. doi: 10.1128/jb.114.1.249-256.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Calmes R., Deal S. J. Glycerol transport by Nocardia asteroides. Can J Microbiol. 1972 Nov;18(11):1703–1708. doi: 10.1139/m72-264. [DOI] [PubMed] [Google Scholar]
  4. Carter G. W., Van Dyke K. A superior counting solution for water-soluble tritiated compounds. Clin Chem. 1971 Jul;17(7):576–580. [PubMed] [Google Scholar]
  5. Frerman F. E., Bennett W. Studies on the uptake of fatty acids by Escherichia coli. Arch Biochem Biophys. 1973 Nov;159(1):434–443. doi: 10.1016/0003-9861(73)90471-2. [DOI] [PubMed] [Google Scholar]
  6. Frerman F. E. The role of acetyl coenzyme A: butyrate coenzyme A in the transferase uptake of butyrate by isolated membrane vesicles of Escherichia coli. Arch Biochem Biophys. 1973 Nov;159(1):444–452. doi: 10.1016/0003-9861(73)90472-4. [DOI] [PubMed] [Google Scholar]
  7. Harold F. M., Baarda J. R. Inhibition of membrane transport in Streptococcus faecalis by uncouplers of oxidative phosphorylation and its relationship to proton conduction. J Bacteriol. 1968 Dec;96(6):2025–2034. doi: 10.1128/jb.96.6.2025-2034.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Harold F. M. Chemiosmotic interpretation of active transport in bacteria. Ann N Y Acad Sci. 1974 Feb 18;227:297–311. doi: 10.1111/j.1749-6632.1974.tb14395.x. [DOI] [PubMed] [Google Scholar]
  9. Harold F. M. Conservation and transformation of energy by bacterial membranes. Bacteriol Rev. 1972 Jun;36(2):172–230. doi: 10.1128/br.36.2.172-230.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaback H. R. Transport studies in bacterial membrane vesicles. Science. 1974 Dec 6;186(4167):882–892. doi: 10.1126/science.186.4167.882. [DOI] [PubMed] [Google Scholar]
  11. Klein K., Steinberg R., Fiethen B., Overath P. Fatty acid degradation in Escherichia coli. An inducible system for the uptake of fatty acids and further characterization of old mutants. Eur J Biochem. 1971 Apr;19(3):442–450. doi: 10.1111/j.1432-1033.1971.tb01334.x. [DOI] [PubMed] [Google Scholar]
  12. Lombardi F. J., Reeves J. P., Short S. A., Kaback H. R. Evaluation of the chemiosmotic interpretation of active transport in bacterial membrane vesicles. Ann N Y Acad Sci. 1974 Feb 18;227:312–327. doi: 10.1111/j.1749-6632.1974.tb14396.x. [DOI] [PubMed] [Google Scholar]
  13. Overath P., Pauli G., Schairer H. U. Fatty acid degradation in Escherichia coli. An inducible acyl-CoA synthetase, the mapping of old-mutations, and the isolation of regulatory mutants. Eur J Biochem. 1969 Feb;7(4):559–574. [PubMed] [Google Scholar]
  14. Pauli G., Overath P. ato Operon: a highly inducible system for acetoacetate and butyrate degradation in Escherichia coli. Eur J Biochem. 1972 Sep 25;29(3):553–562. doi: 10.1111/j.1432-1033.1972.tb02021.x. [DOI] [PubMed] [Google Scholar]
  15. Pavlasova E., Harold F. M. Energy coupling in the transport of beta-galactosides by Escherichia coli: effect of proton conductors. J Bacteriol. 1969 Apr;98(1):198–204. doi: 10.1128/jb.98.1.198-204.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Salanitro J. P., Wegener W. S. Growth of Escherichia coli on short-chain fatty acids: nature of the uptake system. J Bacteriol. 1971 Nov;108(2):893–901. doi: 10.1128/jb.108.2.893-901.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Samuel D., Estroumza J., Ailhaud G. Partial purification and properties of acyl-CoA synthetase of Escherichia coli. Eur J Biochem. 1970 Feb;12(3):576–582. doi: 10.1111/j.1432-1033.1970.tb00889.x. [DOI] [PubMed] [Google Scholar]
  18. Toscano W. A., Jr, Hartline R. A. Transport of octanoate by Pseudomonas oleovorans. J Bacteriol. 1973 Nov;116(2):541–547. doi: 10.1128/jb.116.2.541-547.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Vanderwinkel E., Furmanski P., Reeves H. C., Ajl S. J. Growth of Escherichia coli on fatty acids: requirement for coenzyme A transferase activity. Biochem Biophys Res Commun. 1968 Dec 30;33(6):902–908. doi: 10.1016/0006-291x(68)90397-5. [DOI] [PubMed] [Google Scholar]
  20. Wagner C., Odom R., Briggs W. T. The uptake of acetate by Escherichia coli w. Biochem Biophys Res Commun. 1972 Jun 9;47(5):1036–1043. doi: 10.1016/0006-291x(72)90937-0. [DOI] [PubMed] [Google Scholar]
  21. Weeks G., Shapiro M., Burns R. O., Wakil S. J. Control of fatty acid metabolism. I. Induction of the enzymes of fatty acid oxidation in Escherichia coli. J Bacteriol. 1969 Feb;97(2):827–836. doi: 10.1128/jb.97.2.827-836.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Winkler H. H. Energy coupling of the hexose phosphate transport system in Escherichia coli. J Bacteriol. 1973 Oct;116(1):203–209. doi: 10.1128/jb.116.1.203-209.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

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