Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1976 Apr;126(1):64–71. doi: 10.1128/jb.126.1.64-71.1976

Comparative studies on membrane-associated, folded chromosomes from Escherichia coli.

P Dworsky
PMCID: PMC233260  PMID: 770443

Abstract

Nuclear bodies were isolated from Escherichia coli spheroplasts by two different lysis procedures. Their lipid and protein content and the superhelix density was measured. The preparations differed mainly in respect to the amount of the attached membrane material, which seems to be an essential factor in maintaining the stability of the nuclear bodies. The superhelix density was found to be higher than that of naturally occurring, covalently closed, circular deoxyribonucleic acids. The influence of the preparative method on the superhelicity was comparatively small.

Full text

PDF
64

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauer W., Vinograd J. The interaction of closed circular DNA with intercalative dyes. I. The superhelix density of SV40 DNA in the presence and absence of dye. J Mol Biol. 1968 Apr 14;33(1):141–171. doi: 10.1016/0022-2836(68)90286-6. [DOI] [PubMed] [Google Scholar]
  2. CUMMINGS D. J. SEDIMENTATION AND BIOLOGICAL PROPERTIES OF T-PHAGES OF ESCHERICHIA COLI. Virology. 1964 Jul;23:408–418. doi: 10.1016/0042-6822(64)90264-8. [DOI] [PubMed] [Google Scholar]
  3. Choules G. L., Zimm B. H. An acrylamide gel soluble in scintillation fluids: its application to electrophoresis at neutral and low pH. Anal Biochem. 1965 Nov;13(2):336–344. doi: 10.1016/0003-2697(65)90202-2. [DOI] [PubMed] [Google Scholar]
  4. Cronan J. E., Vagelos P. R. Metabolism and function of the membrane phospholipids of Escherichia coli. Biochim Biophys Acta. 1972 Feb 14;265(1):25–60. doi: 10.1016/0304-4157(72)90018-4. [DOI] [PubMed] [Google Scholar]
  5. Dworsky P. A mild method for the isolation of folded chromosomes from Escherichia coli. Z Allg Mikrobiol. 1975;15(4):231–241. doi: 10.1002/jobm.3630150403. [DOI] [PubMed] [Google Scholar]
  6. Dworsky P. Einfluss von Inhibitoren der Protein- und Nucleinsäuresynthese auf die Gestalt des Nucleoids von Escherichia coli. Z Allg Mikrobiol. 1974;14(1):3–28. doi: 10.1002/jobm.3630140102. [DOI] [PubMed] [Google Scholar]
  7. Dworsky P., Schaechter M. Effect of rifampin on the structure and membrane attachment of the nucleoid of Escherichia coli. J Bacteriol. 1973 Dec;116(3):1364–1374. doi: 10.1128/jb.116.3.1364-1374.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dworsky P. Unfolding of the chromosome of Escherichia coli after treatment with rifampicin. Z Allg Mikrobiol. 1975;15(4):243–247. doi: 10.1002/jobm.3630150404. [DOI] [PubMed] [Google Scholar]
  9. Flink I., Pettijohn D. E. Polyamines stabilise DNA folds. Nature. 1975 Jan 3;253(5486):62–63. doi: 10.1038/253062a0. [DOI] [PubMed] [Google Scholar]
  10. Genest D., Wahl P., Auchet J. C. The fluorescence anisotropy decay due to energy transfers occuring in the ethidium bromide-DNA complex. Determination of the deformation angle of the DNA helix. Biophys Chem. 1974 Apr;1(4):266–278. doi: 10.1016/0301-4622(74)80013-x. [DOI] [PubMed] [Google Scholar]
  11. Gesteland R. F. Isolation and characterization of ribonuclease I mutants of Escherichia coli. J Mol Biol. 1966 Mar;16(1):67–84. doi: 10.1016/s0022-2836(66)80263-2. [DOI] [PubMed] [Google Scholar]
  12. Grossman N., Ron E. Z. Membrane-bound DNA from Escherichia coli: extraction by freeze-thaw-lysozyme. FEBS Lett. 1975 Jul 1;54(3):327–329. doi: 10.1016/0014-5793(75)80932-x. [DOI] [PubMed] [Google Scholar]
  13. Henning U., Haller I. Mutants of Escherichia coli K12 lacking all 'major' proteins of the outer cell envelope membrane. FEBS Lett. 1975 Jul 15;55(1):161–164. doi: 10.1016/0014-5793(75)80983-5. [DOI] [PubMed] [Google Scholar]
  14. Hinton D. M., Bode V. C. Ethidium binding affinity of circular lambda deoxyribonucleic acid determined fluorometrically. J Biol Chem. 1975 Feb 10;250(3):1061–1070. [PubMed] [Google Scholar]
  15. Kornberg T., Lockwood A., Worcel A. Replication of the Escherichia coli chromosome with a soluble enzyme system. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3189–3193. doi: 10.1073/pnas.71.8.3189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LePecq J. B., Paoletti C. A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization. J Mol Biol. 1967 Jul 14;27(1):87–106. doi: 10.1016/0022-2836(67)90353-1. [DOI] [PubMed] [Google Scholar]
  17. Paoletti J., Le Pecq J. B. Resonance energy transfer between ethidium bromide molecules bound to nucleic acids. Does intercalation wind or unwind the DNA helix? J Mol Biol. 1971 Jul 14;59(1):43–62. doi: 10.1016/0022-2836(71)90412-8. [DOI] [PubMed] [Google Scholar]
  18. Pettijohn D. E., Hecht R. RNA molecules bound to the folded bacterial genome stabilize DNA folds and segregate domains of supercoiling. Cold Spring Harb Symp Quant Biol. 1974;38:31–41. doi: 10.1101/sqb.1974.038.01.006. [DOI] [PubMed] [Google Scholar]
  19. Pulleyblank D. E., Morgan A. R. The sense of naturally occurring superhelices and the unwinding angle of intercalated ethidium. J Mol Biol. 1975 Jan 5;91(1):1–13. doi: 10.1016/0022-2836(75)90368-x. [DOI] [PubMed] [Google Scholar]
  20. ROUF M. A. SPECTROCHEMICAL ANALYSIS OF INORGANIC ELEMENTS IN BACTERIA. J Bacteriol. 1964 Dec;88:1545–1549. doi: 10.1128/jb.88.6.1545-1549.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Robberson D. L., Clayton D. A. Replication of mitochondrial DNA in mouse L cells and their thymidine kinase - derivatives: displacement replication on a covalently-closed circular template. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3810–3814. doi: 10.1073/pnas.69.12.3810. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ryder O. A., Smith D. W. Isolation of membrane-associated folded chromosomes from Escherichia coli: effect of protein synthesis inhibition. J Bacteriol. 1974 Dec;120(3):1356–1363. doi: 10.1128/jb.120.3.1356-1363.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Stonington O. G., Pettijohn D. E. The folded genome of Escherichia coli isolated in a protein-DNA-RNA complex. Proc Natl Acad Sci U S A. 1971 Jan;68(1):6–9. doi: 10.1073/pnas.68.1.6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tsai C. C., Jain S. C., Sobell H. M. X-ray crystallographic visualization of drug-nucleic acid intercalative binding: structure of an ethidium-dinucleoside monophosphate crystalline complex, Ethidium: 5-iodouridylyl (3'-5') adenosine. Proc Natl Acad Sci U S A. 1975 Feb;72(2):628–632. doi: 10.1073/pnas.72.2.628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Vinograd J., Lebowitz J., Watson R. Early and late helix-coil transitions in closed circular DNA. The number of superhelical turns in polyoma DNA. J Mol Biol. 1968 Apr 14;33(1):173–197. doi: 10.1016/0022-2836(68)90287-8. [DOI] [PubMed] [Google Scholar]
  26. Wang J. C., Barkley M. D., Bourgeois S. Measurements of unwinding of lac operator by repressor. Nature. 1974 Sep 20;251(5472):247–249. doi: 10.1038/251247a0. [DOI] [PubMed] [Google Scholar]
  27. Wang J. C. The degree of unwinding of the DNA helix by ethidium. I. Titration of twisted PM2 DNA molecules in alkaline cesium chloride density gradients. J Mol Biol. 1974 Nov 15;89(4):783–801. doi: 10.1016/0022-2836(74)90053-9. [DOI] [PubMed] [Google Scholar]
  28. Wang J. C. Variation of the average rotation angle of the DNA helix and the superhelical turns of covalently closed cyclic lambda DNA. J Mol Biol. 1969 Jul 14;43(1):25–39. doi: 10.1016/0022-2836(69)90076-x. [DOI] [PubMed] [Google Scholar]
  29. Weber K., Pringle J. R., Osborn M. Measurement of molecular weights by electrophoresis on SDS-acrylamide gel. Methods Enzymol. 1972;26:3–27. doi: 10.1016/s0076-6879(72)26003-7. [DOI] [PubMed] [Google Scholar]
  30. Worcel A., Burgi E. On the structure of the folded chromosome of Escherichia coli. J Mol Biol. 1972 Nov 14;71(2):127–147. doi: 10.1016/0022-2836(72)90342-7. [DOI] [PubMed] [Google Scholar]
  31. Worcel A., Burgi E. Properties of a membrane-attached form of the folded chromosome of Escherichia coli. J Mol Biol. 1974 Jan 5;82(1):91–105. doi: 10.1016/0022-2836(74)90576-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES