Abstract
Addition of urea to an uninduced culture of Saccharomyces at 22 C results in appearance of allophanate hydrolase activity after a lag of 12 min. We have previously demonstrated that both ribonucleic acid (RNA) and protein synthesis are needed for this induction to occur. To elucidate the time intervals occupied by known processes involved in induction, temperature-sensitive mutants defective in messenger RNA transport from nucleus to cytoplasm (rna1) and in protein synthesis initiation (prt1) were employed along with an RNA polymerase inhibitor in experiments that measure cumulative synthetic capacity to produce allophanate hydrolase. These measurements identify the time within the lag period at which each of the above processes is completed. We observed that RNA synthesis, rna1 gene product function, and protein synthesis initiation are completed at 1 to 1.5, 4, and 9 to 10 min, respectively.
Full text
PDF![198](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/219d/233275/b4576534103e/jbacter00317-0210.png)
![199](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/219d/233275/e0961d4ae3a6/jbacter00317-0211.png)
![200](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/219d/233275/b4fdef2214d4/jbacter00317-0212.png)
![201](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/219d/233275/df3d3b19ae91/jbacter00317-0213.png)
![202](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/219d/233275/1c2cb87ba73d/jbacter00317-0214.png)
![203](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/219d/233275/19fc630349d1/jbacter00317-0215.png)
![204](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/219d/233275/d2574d170dc6/jbacter00317-0216.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cooper T. G., Lawther R. P. Induction of the allantoin degradative enzymes in Saccharomyces cerevisiae by the last intermediate of the pathway. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2340–2344. doi: 10.1073/pnas.70.8.2340. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartwell L. H., Hutchison H. T., Holland T. M., McLaughlin C. S. The effect of cycloheximide upon polyribosome stability in two yeast mutants defective respectively in the initiation of polypeptide chains and in messenger RNA synthesis. Mol Gen Genet. 1970;106(4):347–361. doi: 10.1007/BF00324052. [DOI] [PubMed] [Google Scholar]
- Hartwell L. H., McLaughlin C. S. A mutant of yeast apparently defective in the initiation of protein synthesis. Proc Natl Acad Sci U S A. 1969 Feb;62(2):468–474. doi: 10.1073/pnas.62.2.468. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartwell L. H., McLaughlin C. S. Temperature-sensitive mutants of yeast exhibiting a rapid inhibition of protein synthesis. J Bacteriol. 1968 Nov;96(5):1664–1671. doi: 10.1128/jb.96.5.1664-1671.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KEPES A. KINETICS OF INDUCED ENZYME SYNTHESIS. DETERMINATION OF THE MEAN LIFE OF GALACTOSIDASE-SPECIFIC MESSENGER RNA. Biochim Biophys Acta. 1963 Oct 15;76:293–309. [PubMed] [Google Scholar]
- Lawther R. P., Cooper T. G. Effects of inducer addition and removal upon the level of allophanate hydrolase in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1973 Dec 19;55(4):1100–1104. doi: 10.1016/s0006-291x(73)80008-7. [DOI] [PubMed] [Google Scholar]
- Lawther R. P., Cooper T. G. Kinetics of induced and repressed enzyme synthesis in Saccharomyces cerevisiae. J Bacteriol. 1975 Mar;121(3):1064–1073. doi: 10.1128/jb.121.3.1064-1073.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lawther R. P., Phillips S. L., Cooper T. G. Lomofungin inhibition of allophanate hydrolase synthesis in Saccharomyces cerevisiae. Mol Gen Genet. 1975;137(2):89–99. doi: 10.1007/BF00341675. [DOI] [PubMed] [Google Scholar]
- Shiokawa K., Pogo A. O. The role of cytoplasmic membranes in controlling the transport of nuclear messenger RNA and initiation of protein synthesis. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2658–2662. doi: 10.1073/pnas.71.7.2658. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Whitney P. A., Cooper T. G., Magasanik B. The induction of urea carboxylase and allophanate hydrolase in Saccharomyces cerevisiae. J Biol Chem. 1973 Sep 10;248(17):6203–6209. [PubMed] [Google Scholar]
- Whitney P. A., Cooper T. G. Urea carboxylase and allophanate hydrolase. Two components of adenosine triphosphate:urea amido-lyase in Saccharomyces cerevisiae. J Biol Chem. 1972 Mar 10;247(5):1349–1353. [PubMed] [Google Scholar]
- Wickerham L. J. A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts. J Bacteriol. 1946 Sep;52(3):293–301. [PMC free article] [PubMed] [Google Scholar]