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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1976 Apr;126(1):198–204. doi: 10.1128/jb.126.1.198-204.1976

Sequence of molecular events involved in induction of allophanate hydrolase.

J Bossinger, T G Cooper
PMCID: PMC233275  PMID: 944180

Abstract

Addition of urea to an uninduced culture of Saccharomyces at 22 C results in appearance of allophanate hydrolase activity after a lag of 12 min. We have previously demonstrated that both ribonucleic acid (RNA) and protein synthesis are needed for this induction to occur. To elucidate the time intervals occupied by known processes involved in induction, temperature-sensitive mutants defective in messenger RNA transport from nucleus to cytoplasm (rna1) and in protein synthesis initiation (prt1) were employed along with an RNA polymerase inhibitor in experiments that measure cumulative synthetic capacity to produce allophanate hydrolase. These measurements identify the time within the lag period at which each of the above processes is completed. We observed that RNA synthesis, rna1 gene product function, and protein synthesis initiation are completed at 1 to 1.5, 4, and 9 to 10 min, respectively.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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