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. 1976 Apr;126(1):300–311. doi: 10.1128/jb.126.1.300-311.1976

Defective synthesis of lipid intermediates for peptidoglycan formation in a stabilized L-form of Streptococcus pyogenes.

V M Reusch, C Panos
PMCID: PMC233288  PMID: 4426

Abstract

Membrane preparations obtained from a stabilized L-form of Streptococcus pyogenes are incapable of synthesizing peptidoglycan from uridine-5'-diphospho-N-acetyl-D-muramyl-L-Ala-D-iso-Glu-L-Lys-D-Ala-D-Ala and uridine-5'-diphospho-N-acetyl-D-glucosamine, in contrast with similar preparations from the parental streptococcus. Furthermore, 50-fold higher levels of lipid intermediates which serve as membrane-bound substrates for peptidoglycan synthesis are synthesized in reaction mixtures containing streptococcal membranes than with similar preparations from the L-form. These observations suggest that the inability of this stabilized L-form to form a cell wall in vivo lies, at least in part, in its failure to synthesize significant quantities of the lipid substrates for peptidoglycan synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson J. S., Matsuhashi M., Haskin M. A., Strominger J. L. Biosythesis of the peptidoglycan of bacterial cell walls. II. Phospholipid carriers in the reaction sequence. J Biol Chem. 1967 Jul 10;242(13):3180–3190. [PubMed] [Google Scholar]
  2. Anderson J. S., Meadow P. M., Haskin M. A., Strominger J. L. Biosynthesis of the peptidoglycan of bacterial cell walls. I. Utilization of uridine diphosphate acetylmuramyl pentapeptide and uridine diphosphate acetylglucosamine for peptidoglycan synthesis by particulate enzymes from Staphylococcus aureus and Micrococcus lysodeikticus. Arch Biochem Biophys. 1966 Sep 26;116(1):487–515. doi: 10.1016/0003-9861(66)90056-7. [DOI] [PubMed] [Google Scholar]
  3. Anderson R. G., Hussey H., Baddiley J. The mechanism of wall synthesis in bacteria. The organization of enzymes and isoprenoid phosphates in the membrane. Biochem J. 1972 Mar;127(1):11–25. doi: 10.1042/bj1270011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blumberg P. M., Strominger J. L. Interaction of penicillin with the bacterial cell: penicillin-binding proteins and penicillin-sensitive enzymes. Bacteriol Rev. 1974 Sep;38(3):291–335. doi: 10.1128/br.38.3.291-335.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chatterjee A. N., Ward J. B., Perkins H. R. Synthesis of mucopeptide by L-form membranes. Nature. 1967 Jun 24;214(5095):1311–1314. doi: 10.1038/2141311a0. [DOI] [PubMed] [Google Scholar]
  6. Cohen M., Panos C. Cell wall polysaccharide biosynthesis by membrane fragments from Streptococcus pyogenes and stabilized L-form. J Bacteriol. 1971 May;106(2):347–355. doi: 10.1128/jb.106.2.347-355.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Edwards J., Panos C. STREPTOCOCCAL L FORMS V. : Acid-Soluble Nucleotides of a Group A Streptococcus and Derived L Form. J Bacteriol. 1962 Dec;84(6):1202–1208. doi: 10.1128/jb.84.6.1202-1208.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goldman R., Strominger J. L. Purification and properties of C 55 -isoprenylpyrophosphate phosphatase from Micrococcus lysodeikticus. J Biol Chem. 1972 Aug 25;247(16):5116–5122. [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Munoz E., Ghuysen J. M., Heymann H. Cell walls of Streptococcus pyogenes, type 14. C polysaccharide-peptidoglycan and G polysaccharide-peptidoglycan complexes. Biochemistry. 1967 Dec;6(12):3659–3670. doi: 10.1021/bi00864a007. [DOI] [PubMed] [Google Scholar]
  11. NEUHAUS F. C., STRUVE W. G. ENZYMATIC SYNTHESIS OF ANALOGS OF THE CELL-WALL PRECURSOR. I. KINETICS AND SPECIFICITY OF URIDINE DIPHOSPHO-N-ACETYLMURAMYL-L-ALANYL-D-GLUTAMYL-L-LYSINE:D-ALANYL-D-ALANINE LIGASE (ADENOSINE DIPHOSPHATE) FROM STREPTOCOCCUS FAECALIS R. Biochemistry. 1965 Jan;4:120–131. doi: 10.1021/bi00877a020. [DOI] [PubMed] [Google Scholar]
  12. PANOS C., BARKULIS S. S., HAYASHI J. A. Streptococcal L forms. II. Chemical composition. J Bacteriol. 1959 Dec;78:863–867. doi: 10.1128/jb.78.6.863-867.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. PATTERSON M. S., GREENE R. C. MEASUREMENT OF LOW ENERGY BETA-EMITTERS IN AQUEOUS SOLUTION BY LIQUID SCINTILLATION COUNTING OF EMULSIONS. Anal Chem. 1965 Jun;37:854–857. doi: 10.1021/ac60226a017. [DOI] [PubMed] [Google Scholar]
  14. Pandhi P. N., Panos C. Biosynthesis of D-alanyl-D-alanine from L-alanine by extracts of a stabilized L-form from Streptococcus pyogenes. J Gen Microbiol. 1972 Aug;71(3):487–494. doi: 10.1099/00221287-71-3-487. [DOI] [PubMed] [Google Scholar]
  15. Panos C., Cohen M. Cell wall inhibition in a stable streptococcal L-form. Biochim Biophys Acta. 1966 Mar 28;117(1):98–106. doi: 10.1016/0304-4165(66)90156-5. [DOI] [PubMed] [Google Scholar]
  16. Petit J. F., Strominger J. L., Söll D. Biosynthesis of the peptidoglycan of bacterial cell walls. VII. Incorporation of serine and glycine into interpeptide bridges in Staphylococcus epidermidis. J Biol Chem. 1968 Feb 25;243(4):757–767. [PubMed] [Google Scholar]
  17. Reusch V. M., Jr, Burger M. M. Distribution of marker enzymes between mesosomal and protoplast membranes. J Biol Chem. 1974 Aug 25;249(16):5337–5345. [PubMed] [Google Scholar]
  18. Sandermann H., Jr, Strominger J. L. Purification and properties of C 55 -isoprenoid alcohol phosphokinase from Staphylococcus aureus. J Biol Chem. 1972 Aug 25;247(16):5123–5131. [PubMed] [Google Scholar]
  19. Slabyj B. M., Panos C. Teichoic acid of a stabilized L-form of Streptococcus pyogenes. J Bacteriol. 1973 Jun;114(3):934–942. doi: 10.1128/jb.114.3.934-942.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Willoughby E., Highasi Y., Strominger J. L. Enzymatic dephosphorylation of C 55 -isoprenylphosphate. J Biol Chem. 1972 Aug 25;247(16):5113–5115. [PubMed] [Google Scholar]

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