Abstract
Antiserum prepared against protocatechuate 3, 4-dioxygenase from Pseudomonas aeruginosa forms precipitin bands without spurs with isofunctional enzymes from different strains of the fluorescent pseudomonads on immunodiffusion plates. Catalytic activity of the isofunctional enzymes was inhibited by an immunoglobulin fraction prepared against the enzyme from organisms of the same genus and not from different genera.
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- Fujisawa H., Hayaishi O. Protocatechuate 3,4-dioxygenase. I. Crystallization and characterization. J Biol Chem. 1968 May 25;243(10):2673–2681. [PubMed] [Google Scholar]
- Hou C. T. Circular dichroism of holo- and apoprotocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa. Biochemistry. 1975 Aug 26;14(17):3899–3902. doi: 10.1021/bi00688a025. [DOI] [PubMed] [Google Scholar]
- Kekwick R. A. The serum proteins in multiple myelomatosis. Biochem J. 1940 Sep;34(8-9):1248–1257. doi: 10.1042/bj0341248. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MACDONALD D. L., STANIER R. Y., INGRAHAM J. L. The enzymatic formation of beta-carboxymuconic acid. J Biol Chem. 1954 Oct;210(2):809–820. [PubMed] [Google Scholar]
- Meagher R. B., Ornston L. N. Relationships among enzymes of the beta-ketoadipate pathway. I. Properties of cis,cis-muconate-lactonizing enzyme and muconolactone isomerase from Pseudomonas putida. Biochemistry. 1973 Aug 28;12(18):3523–3530. doi: 10.1021/bi00742a027. [DOI] [PubMed] [Google Scholar]
- OUCHTERLONY O. Antigen-antibody reactions in gels. IV. Types of reactions in coordinated systems of diffusion. Acta Pathol Microbiol Scand. 1953;32(2):230–240. [PubMed] [Google Scholar]
- Patel R. N., Meagher R. B., Ornston L. N. Relationships among enzymes of the beta-ketoadipate pathway. IV. Muconolactone isomerase from Acinetobacter calcoaceticus and Pseudomonas putida. J Biol Chem. 1974 Dec 10;249(23):7410–7419. [PubMed] [Google Scholar]
- STANIER R. Y., INGRAHAM J. L. Protocatechuic acid oxidase. J Biol Chem. 1954 Oct;210(2):799–808. [PubMed] [Google Scholar]
- Stanier R. Y., Wachter D., Gasser C., Wilson A. C. Comparative immunological studies of two Pseudomonas enzymes. J Bacteriol. 1970 May;102(2):351–362. doi: 10.1128/jb.102.2.351-362.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]


