Abstract
With polyacrylamide disc gel electrophoresis and specific staining, it was demonstrated that one mutation involving the alcohol dehydrogenase of a double mutant of Bacillus stearothermophilus 1503 apparently prevented enzyme synthesis, and another lesion in the same organism resulted in synthesis of an inactive form of aconitase. Some properties of the double mutant and two fumarase mutants are discussed in relation to similar mutants derived from Bacillus subtilis.
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- Amarasingham C. R., Davis B. D. Regulation of alpha-ketoglutarate dehydrogenase formation in Escherichia coli. J Biol Chem. 1965 Sep;240(9):3664–3668. [PubMed] [Google Scholar]
- Carls R. A., Hanson R. S. Isolation and characterization of tricarboxylic acid cycle mutants of Bacillus subtilis. J Bacteriol. 1971 Jun;106(3):848–855. doi: 10.1128/jb.106.3.848-855.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cuatrecasas P., Segal S. "Galactose dehydrogenase", "nothing dehydrogenase", and alcohol dehydrogenase: interrelation. Science. 1967 Jun 16;156(3781):1518–1518. [PubMed] [Google Scholar]
- Fox D. J. The soluble citric acid cycle enzymes of Drosophila melanogaster. I. Genetics and ontogeny of NADP-linked isocitrate dehydrogenase. Biochem Genet. 1971 Feb;5(1):69–80. doi: 10.1007/BF00485732. [DOI] [PubMed] [Google Scholar]
- Ohné M. Regulation of aconitase synthesis in Bacillus subtilis: induction, feedback repression, and catabolite repression. J Bacteriol. 1974 Mar;117(3):1295–1305. doi: 10.1128/jb.117.3.1295-1305.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ohné M. Regulation of the dicarboxylic acid part of the citric acid cycle in Bacillus subtilis. J Bacteriol. 1975 Apr;122(1):224–234. doi: 10.1128/jb.122.1.224-234.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rowe J. J., Goldberg I. D., Amelunxen R. E. Development of defined and minimal media for the growth of Bacillus stearothermophilus. J Bacteriol. 1975 Oct;124(1):279–284. doi: 10.1128/jb.124.1.279-284.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rowe J. J., Goldberg I. D., Amelunxen R. E. Isolation of mutants of Bacillus stearothermophilus blocked in catabolic function. Can J Microbiol. 1973 Dec;19(12):1521–1523. doi: 10.1139/m73-249. [DOI] [PubMed] [Google Scholar]
- Shaw C. R., Prasad R. Starch gel electrophoresis of enzymes--a compilation of recipes. Biochem Genet. 1970 Apr;4(2):297–320. doi: 10.1007/BF00485780. [DOI] [PubMed] [Google Scholar]
- THORNE C. B. Transduction in Bacillus subtilis. J Bacteriol. 1962 Jan;83:106–111. doi: 10.1128/jb.83.1.106-111.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]