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. 1976 Jan;125(1):33–41. doi: 10.1128/jb.125.1.33-41.1976

Genetic co-regulation of galactose and melibiose utilization in Saccharomyces.

O M Kew, H C Douglas
PMCID: PMC233332  PMID: 1245460

Abstract

The gal3 mutation of Saccharomyces, which is associated with an impairment in the utilization of galactose, has been shown to be pleiotropic, causing similar impairments in the utilization of melibiose and maltose. Milibiose utilization and alpha-galactosidase production are directly controlled by the galactose regulatory elements i, c, and GAL4. The fermentation of maltose and the induction of alpha-glucosidase are regulated independently of the i, c, GAL4 system. The production of alpha-galactosidase and galactose-1-phosphate uridyl transferase is coordinate in galactokinaseless strains. Galactose serves as a nonmetabolized, gratuitous inducer of alpha-galactosidase in strains lacking the genes for one or more of the Leloir pathway enzymes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AVIGAD G. Accumulation of trehalose and sucrose in relation to the metabolism of alpha-glucosides in yeasts of defined genotype. Biochim Biophys Acta. 1960 May 6;40:124–134. doi: 10.1016/0006-3002(60)91322-6. [DOI] [PubMed] [Google Scholar]
  2. Adams B. G. Method for decryptification of -glucosidase in yeast with dimethyl sulfoxide. Anal Biochem. 1972 Jan;45(1):137–146. doi: 10.1016/0003-2697(72)90014-0. [DOI] [PubMed] [Google Scholar]
  3. BUTTIN G. M'ECANISMES R'EGULATEURS DANS LA BIOSYNTH'ESE DES ENZYMES DU M'ETABOLISME DU GALACTOSE CHEZ ESCHERICHIA COLI K12. I. LA BIOSYNTH'ESE INDUITE DE LA GALACTOKINASE ET L'INDUCTION SIMULTAN'EE DE LA S'EQUENCE ENZYMATIQUE. J Mol Biol. 1963 Aug;7:164–182. doi: 10.1016/s0022-2836(63)80044-3. [DOI] [PubMed] [Google Scholar]
  4. Bassel J., Mortimer R. Genetic order of the galactose structural genes in Saccharomyces cerevisiae. J Bacteriol. 1971 Oct;108(1):179–183. doi: 10.1128/jb.108.1.179-183.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bevan P., Douglas H. C. Genetic control of phosphoglucomutase variants in Saccharomyces cerevisiae. J Bacteriol. 1969 May;98(2):532–535. doi: 10.1128/jb.98.2.532-535.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cirillo V. P. Galactose transport in Saccharomyces cerevisiae. I. Nonmetabolized sugars as substrates and inducers of the galactose transport system. J Bacteriol. 1968 May;95(5):1727–1731. doi: 10.1128/jb.95.5.1727-1731.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DOUGLAS H. C. A mutation in saccharomyces that affects phosphoglucomutase activity and galactose utilization. Biochim Biophys Acta. 1961 Sep 2;52:209–211. doi: 10.1016/0006-3002(61)90924-6. [DOI] [PubMed] [Google Scholar]
  8. DOUGLAS H. C., CONDIE F. The genetic control of galactose utilization in Saccharomyces. J Bacteriol. 1954 Dec;68(6):662–670. doi: 10.1128/jb.68.6.662-670.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. DOUGLAS H. C., HAWTHORNE D. C. ENZYMATIC EXPRESSION AND GENETIC LINKAGE OF GENES CONTROLLING GALACTOSE UTILIZATION IN SACCHAROMYCES. Genetics. 1964 May;49:837–844. doi: 10.1093/genetics/49.5.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Douglas H. C., Hawthorne C. D. Uninducible mutants in the gal i locus of Saccharomyces cerevisiae. J Bacteriol. 1972 Mar;109(3):1139–1143. doi: 10.1128/jb.109.3.1139-1143.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Douglas H. C., Hawthorne D. C. Regulation of genes controlling synthesis of the galactose pathway enzymes in yeast. Genetics. 1966 Sep;54(3):911–916. doi: 10.1093/genetics/54.3.911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. FRIIS J., OTTOLENGHI P. Localization of melibiase in a strain of veast. C R Trav Lab Carlsberg. 1959;31:272–281. [PubMed] [Google Scholar]
  13. HALVORSON H. O., WINDERMAN S., GORMAN J. Comparison of the alpha-glucosidases of Saccharomyces produced in response to five non-allelic maltose genes. Biochim Biophys Acta. 1963 Jan 8;67:42–53. doi: 10.1016/0006-3002(63)91795-5. [DOI] [PubMed] [Google Scholar]
  14. HAUPT W., ALPS H. [On the fermentation of melibiose by Saccaharomyces strains]. Arch Mikrobiol. 1963;45:179–187. [PubMed] [Google Scholar]
  15. Khan N. A., Eaton N. R. Genetic control of maltase formation in yeast. I. Strains producing high and low basal levels of enzyme. Mol Gen Genet. 1971;112(4):317–322. doi: 10.1007/BF00334433. [DOI] [PubMed] [Google Scholar]
  16. Khan N. A., Zimmermann F. K., Eaton N. R. Genetic control of maltase formation in yeast. II. Evidence for a gene regulating the level of maltase production. Mol Gen Genet. 1973 Aug 28;124(4):365–367. doi: 10.1007/BF00267665. [DOI] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. Lindegren C. C., Spiegelman S., Lindegren G. Mendelian Inheritance of Adaptive Enzymes. Proc Natl Acad Sci U S A. 1944 Nov 15;30(11):346–352. doi: 10.1073/pnas.30.11.346. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SPIEGELMAN S., SUSSMAN R. R., PINSKA E. On the cytoplasmic nature of "long-term adaptation" in yeast. Proc Natl Acad Sci U S A. 1950 Nov;36(11):591–606. doi: 10.1073/pnas.36.11.591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tsuyumu S., Adams B. G. Dilution kinetic studies of yeast populations: in vivo aggregation of galactose utilizing enzymes and positive regulator molecules. Genetics. 1974 Jul;77(3):491–505. doi: 10.1093/genetics/77.3.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tsuyumu S., Adams B. G. Population analysis of the deinduction kinetics of galactose long-term adaptation mutants of yeast. Proc Natl Acad Sci U S A. 1973 Mar;70(3):919–923. doi: 10.1073/pnas.70.3.919. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. WINGE O., ROBERTS C. A genetic analysis of melibiose and raffinose fermentation. Cr Trav Lab Carlsberg Ser Physiol. 1957;25(18-19):419–459. [PubMed] [Google Scholar]
  23. Zimmermann F. K., Khan N. A., Eaton N. R. Identification of new genes involved in disaccharide fermentation in yeast. Mol Gen Genet. 1973;123(1):29–41. doi: 10.1007/BF00282986. [DOI] [PubMed] [Google Scholar]
  24. ten Berge A. M. Genes for the fermentation of maltose and -methylglucoside in Saccharomyces carlsbergensis. Mol Gen Genet. 1972;115(1):80–88. doi: 10.1007/BF00272220. [DOI] [PubMed] [Google Scholar]
  25. ten Berge A. M., Zoutewelle G., van de Poll K. W., Bloemers H. P. Regulation of maltose fermentation in Saccharomyces carlsbergensis. II. Properties of a constitutive MAL6-mutant. Mol Gen Genet. 1973 Sep 5;125(2):139–146. doi: 10.1007/BF00268867. [DOI] [PubMed] [Google Scholar]
  26. ten Berge A. M., Zoutewelle G., van de Poll K. W. Regulation of maltose fermentation in Saccharomyces carlsbergensis. I. The function of the gene MAL6, as recognized by mal6-mutants. Mol Gen Genet. 1973 Jul 2;123(3):233–246. doi: 10.1007/BF00271242. [DOI] [PubMed] [Google Scholar]

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