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. 1976 Jan;125(1):74–83. doi: 10.1128/jb.125.1.74-83.1976

Evolution of the transcription complex during sporulation of Bacillus subtilis.

J Brevet
PMCID: PMC233337  PMID: 812865

Abstract

Ribonucleic acid polymerase activity in partially purified extract of cells of Bacillus subtilis harvested at different times (t-1, to, t1, and t2) was studied by zone centrifugation. During the course of sporulation, vegetative sigma-factor activity decreased and the transcription complex lost some of its affinity for active sigma factor. The complex underwent a two-stage change in sedimentation value, from 14.5S in vegetative growth phase to a 13S species very early in sporulation to a 16S species at later times. Two SpoO mutants have been studied by zone centrifugation. One strain, a rifampin-resistant (RfmR) mutant, failed to show any modification of the transcription complex, whereas the other, a Rfms strain, underwent a partial evolution of the transcription complex after to.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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